Exogenous melatonin (MT) enhances salt tolerance of okra (Abelmoschus esculentus L.) plants by regulating proline, photosynthesis, ion homeostasis and ROS pathways

Wang, Feibing; Wan, Chenzhong; Wu, W.; Yang, Shasha; Chen, Xinhong

doi:10.1007/s42535-023-00568-7

Cited by 6 publications

(4 citation statements)

References 71 publications

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“…MT may protect antioxidant enzymes from oxidative damage by enhancing the efficiency of the mitochondrial electron transport chain, lowering electron leakage, and reducing free radical production [43]. Exogenous MT application's role has been reported in oxidative stress, redox signaling, ion homeostasis, and enzyme regulation in rice, okra, soybean, cucumber, tea, and maize crops, resulting in reduced oxidative damage under salt stress conditions when compared to control [21,[43][44][45][46][47]. Ascorbic acid and APX are the major antioxidants that play a crucial role in resistance accumulation under stress conditions.…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals the Protective Role of Melatonin during Salt Stress by Regulating the Photosynthesis and Ascorbic Acid Metabolism Pathways in Brassica campestris

Shah,

Wang,

et al. 2024

IJMS

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Salinity stress is a type of abiotic stress which negatively affects the signaling pathways and cellular compartments of plants. Melatonin (MT) has been found to be a bioactive compound that can mitigate these adverse effects, which makes it necessary to understand the function of MT and its role in salt stress. During this study, plants were treated exogenously with 100 µM of MT for 7 days and subjected to 200 mM of salt stress, and samples were collected after 1 and 7 days for different indicators and transcriptome analysis. The results showed that salt reduced chlorophyll contents and damaged the chloroplast structure, which was confirmed by the downregulation of key genes involved in the photosynthesis pathway after transcriptome analysis and qRT-PCR confirmation. Meanwhile, MT increased the chlorophyll contents, reduced the electrolyte leakage, and protected the chloroplast structure during salt stress by upregulating several photosynthesis pathway genes. MT also decreased the H2O2 level and increased the ascorbic acid contents and APX activity by upregulating genes involved in the ascorbic acid pathway during salt stress, as confirmed by the transcriptome and qRT-PCR analyses. Transcriptome profiling also showed that 321 and 441 DEGs were expressed after 1 and 7 days of treatment, respectively. The KEGG enrichment analysis showed that 76 DEGs were involved in the photosynthesis pathway, while 35 DEGs were involved in the ascorbic acid metabolism pathway, respectively. These results suggest that the exogenous application of MT in plants provides important insight into understanding MT-induced stress-responsive mechanisms and protecting Brassica campestris against salt stress by regulating the photosynthesis and ascorbic acid pathway genes.

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Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals the Protective Role of Melatonin during Salt Stress by Regulating the Photosynthesis and Ascorbic Acid Metabolism Pathways in Brassica campestris

Shah,

Wang,

et al. 2024

IJMS

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“…However, the role of JA in plant salt tolerance mechanism varies among species. For instance, both exogenous JA-Me and the accumulation of endogenous JA enhance the salt tolerance of Abelmoschus esculentus ( Wang et al., 2023 ). Conversely, in Oryza sativa , the accumulation of endogenous JA and its signal transduction negatively affected salt tolerance ( Ndecky et al., 2023 ).…”

Section: Discussionmentioning

confidence: 99%

Divergent effects of single and combined stress of drought and salinity on the physiological traits and soil properties of Platycladus orientalis saplings

Li,

Lu,

Wang

et al. 2024

Front. Plant Sci.

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Drought and salinity are two abiotic stresses that affect plant productivity. We exposed 2-year-old Platycladus orientalis saplings to single and combined stress of drought and salinity. Subsequently, the responses of physiological traits and soil properties were investigated. Biochemical traits such as leaf and root phytohormone content significantly increased under most stress conditions. Single drought stress resulted in significantly decreased nonstructural carbohydrate (NSC) content in stems and roots, while single salt stress and combined stress resulted in diverse response of NSC content. Xylem water potential of P. orientalis decreased significantly under both single drought and single salt stress, as well as the combined stress. Under the combined stress of drought and severe salt, xylem hydraulic conductivity significantly decreased while NSC content was unaffected, demonstrating that the risk of xylem hydraulic failure may be greater than carbon starvation. The tracheid lumen diameter and the tracheid double wall thickness of root and stem xylem was hardly affected by any stress, except for the stem tracheid lumen diameter, which was significantly increased under the combined stress. Soil ammonium nitrogen, nitrate nitrogen and available potassium content was only significantly affected by single salt stress, while soil available phosphorus content was not affected by any stress. Single drought stress had a stronger effect on the alpha diversity of rhizobacteria communities, and single salt stress had a stronger effect on soil nutrient availability, while combined stress showed relatively limited effect on these soil properties. Regarding physiological traits, responses of P. orientalis saplings under single and combined stress of drought and salt were diverse, and effects of combined stress could not be directly extrapolated from any single stress. Compared to single stress, the effect of combined stress on phytohormone content and hydraulic traits was negative to P. orientalis saplings, while the combined stress offset the negative effects of single drought stress on NSC content. Our study provided more comprehensive information on the response of the physiological traits and soil properties of P. orientalis saplings under single and combined stress of drought and salt, which would be helpful to understand the adapting mechanism of woody plants to abiotic stress.

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“…[30][31][32] Importantly, application of exogenous melatonin has been found to confer stress tolerance in crops 33 such as okra and broccoli. 34,35 Comparative physiological and transcriptomic studies have shown that exogenous application of melatonin confers salt tolerance in okra (Abelmoschus esculentus L.). 36 In another study, Wang et al 35 showed that external application of melatonin enhances salt tolerance of okra plants by regulating proline, photosynthesis, ion homeostasis and ROS.…”

Section: Melatonin and Abiotic Stressmentioning

confidence: 99%

“…Importantly, application of exogenous melatonin has been found to confer stress tolerance in crops 33 such as okra and broccoli 34,35 . Comparative physiological and transcriptomic studies have shown that exogenous application of melatonin confers salt tolerance in okra ( Abelmoschus esculentus L.) 36 .…”

Section: Introductionmentioning

confidence: 99%

Novel melatonin‐producing Bacillus safensis EH143 mitigates salt and cadmium stress in soybean

Kwon,

Adhikari,

Imran

et al. 2024

Journal of Pineal Research

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Recently, microorganism and exogenous melatonin application has been recognized as an efficient biological tool for enhancing salt tolerance and heavy metal detoxification in agriculture crops. Thus, the goal of this study was to isolate and evaluate a novel melatonin‐producing plant growth promoting bacterium. With high‐throughput whole genome sequencing, phytohormone measurements, expression profiling, and biochemical analysis, we can identify a novel PGPB that produces melatonin and unravel how it promotes soybean growth and development and protects against salt and Cd stress. We identify the melatonin synthesis pathway (tryptophan→tryptamine→serotonin melatonin) of the halotolerant (NaCl > 800 mM) and heavy metal‐resistant (Cd >3 mM) rhizobacterium Bacillus safensis EH143 and use it to treat soybean plants subjected to Cd and NaCl stresses. Results show that EH143 will highly bioaccumulate heavy metals and significantly improve P and Ca2+ uptake and the K+/Na+ (93%↑under salt stress) ratio while reducing Cd uptake (49% under Cd stress) in shoots. This activity was supported by the expression of the ion regulator HKT1, MYPB67, and the calcium sensors CDPK5 and CaMK1 which ultimately led to increased plant growth. EH143 significantly decreased ABA content in shoots by 13%, 20%, and 34% and increased SA biosynthesis in shoots by 14.8%, 31%, and 48.2% in control, salt, and Cd‐treated plants, upregulating CYP707A1 and CYP707A2 and PAL1 and ICS, respectively. The melatonin content significantly decreased along with a reduced expression of ASMT3 following treatment with EH143; moreover, reduced expression of peroxidase (POD) and superoxide dismutase (SOD) by 134.5% and 39% under salt+Cd stress, respectively and increased level of total amino acids were observed. Whole‐genome sequencing and annotation of EH143 revealed the presence of the melatonin precursor tryptophan synthase (trpA, trpB, trpS), metal and other ion regulators (Cd: cadA, potassium: KtrA and KtrB, phosphate: glpT, calcium: yloB, the sodium/glucose cotransporter: sgIT, and the magnesium transporter: mgtE), and enzyme activators (including the siderophore transport proteins yfiZ and yfhA, the SOD sodA, the catalase katA1, and the glutathione regulator KefG) that may be involved in programming the plant metabolic system. As a consequence, EH143 treatment significantly reduced the contents of lipid peroxidation (O2‐, MDA, and H2O2) up to 69%, 46%, and 29% in plants under salt+Cd stress, respectively. These findings suggest that EH143 could be a potent biofertilizer to alleviate NaCl and Cd toxicity in crops and serve as an alternative substitute for exogenous melatonin application.

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Exogenous melatonin (MT) enhances salt tolerance of okra (Abelmoschus esculentus L.) plants by regulating proline, photosynthesis, ion homeostasis and ROS pathways

Cited by 6 publications

References 71 publications

Comparative Transcriptome Analysis Reveals the Protective Role of Melatonin during Salt Stress by Regulating the Photosynthesis and Ascorbic Acid Metabolism Pathways in Brassica campestris

Comparative Transcriptome Analysis Reveals the Protective Role of Melatonin during Salt Stress by Regulating the Photosynthesis and Ascorbic Acid Metabolism Pathways in Brassica campestris

Divergent effects of single and combined stress of drought and salinity on the physiological traits and soil properties of Platycladus orientalis saplings

Novel melatonin‐producing Bacillus safensis EH143 mitigates salt and cadmium stress in soybean

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