Exogenous melatonin enhances salt stress tolerance in maize seedlings by improving antioxidant and photosynthetic capacity

Chen, Yang-Er; Mao, Jingjing; Sun, Liangqi; Huang, Bo; Ding, Chunbang; Gu, Yu; Liao, Jinqiu; Hu, Chao; Zhang, Zhongwei; Yuan, Shu; Yuan, Ming

doi:10.1111/ppl.12737

Cited by 220 publications

(145 citation statements)

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“…Similarly, Sumaira et al (2017) suggested the inducing role of melatonin in the biosynthesis of various phytochemicals [54]. Herein, similar observations were documented, which involve augmentation in the synthesis of phenolic compounds in calli exposed to melatonin [29,42,43,45]. A similar trend was observed for UV-C treatments.…”

Section: Quantification Of Phenylpropanoid Metabolites Via Hplcsupporting

confidence: 77%

“…However, the mechanism of action is not clearly elucidated yet, but research has shown that UV stress is encountered in plants by modulating key enzymes productions, such as CHS (chalcone synthase), PAL (phenylalanine ammonia-lyase) and through the rapid accumulation of phenolic compounds [39][40][41]. Exogenous applications of melatonin affect plants' physiological processes [42,43]. The effects of different melatonin concentrations on purple basil callus cultures were also studied for flavonoids and phenolics production.…”

Section: Influence Uv-c Radiations and Melatonin On Total Phenolic Anmentioning

confidence: 99%

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Interactive Effect of Melatonin and UV-C on Phenylpropanoid Metabolite Production and Antioxidant Potential in Callus Cultures of Purple Basil (Ocimum basilicum L. var purpurascens)

et al. 2020

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The present study evaluated the interactive effect of melatonin and UV-C on phenylpropanoid metabolites profile and antioxidant potential of Ocimum basilicum L. Callus was treated with varying concentrations of melatonin and UV-C radiations for different time durations, either alone and/or in combination. Individual treatments of both UV-C and melatonin proved to be more effective than combine treatments. Results indicated that UV-C (10 min) exposure increased rosmarinic acid (134.5 mg/g dry weight (DW)), which was 2.3-fold greater than control. Chichoric acid (51.52 mg/g DW) and anthocyanin (cyanide 0.50 mg/g DW) were almost 4.1-fold, while peonidin was found 2.7-fold higher in UV-C (50 min) exposure. In the case of melatonin, 1.0 mg/L concentrations showed maximum rosmarinic acid (79.4 mg/g DW) accumulation; i.e., 1.4-fold more, as compared to the control. However, 2 mg/L melatonin accumulate chichoric acid (39.99 mg/g DW) and anthocyanin (cyanide: 0.45 mg/g DW and peonidin: 0.22 mg/g DW); i.e., 3.2, 3.7 and 2.0-fold increase, as compared to the control, respectively. On the other hand, melatonin-combined treatment (melatonin (Mel) (4 mg/L) + UV-C (20 min)) was proved to be effective in caffeic acid elicitation, which was 1.9-fold greater than the control. Furthermore, antioxidant potential was evaluated by both in vitro (DPPH, ABTS and FRAP assays) and in cellulo methods. Maximum in vitro antioxidant activity (DPPH: 90.6% and ABTS: 1909.5 µM) was observed for UV-C (50 min)-treated cultures. The highest in vitro antioxidant activity measured with the ABTS assay as compared to the FRAP assay, suggesting the main contribution of antioxidants from basil callus extracts acting through a hydrogen atom transfer (HAT) over an electron transfer (ET)-based mechanism. Cellular antioxidant assay was evaluated by production of ROS/RNS species using yeast cell cultures and further confirmed the protective action of the corresponding callus extracts against oxidative stress. Overall, both melatonin and UV-C are here proved to be effective elicitors since a positive correlation between the induced production of phenolic compounds, and in cellulo antioxidant action of basil callus extracts were observed.

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Section: Quantification Of Phenylpropanoid Metabolites Via Hplcsupporting

confidence: 77%

Section: Influence Uv-c Radiations and Melatonin On Total Phenolic Anmentioning

confidence: 99%

Interactive Effect of Melatonin and UV-C on Phenylpropanoid Metabolite Production and Antioxidant Potential in Callus Cultures of Purple Basil (Ocimum basilicum L. var purpurascens)

et al. 2020

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“…However, the main function of melatonin on enzymatic or non-enzymatic system-mediated ROS scavenging in alfalfa plants still requires further study. The positive effects of melatonin on antioxidant enzymes system under salt stress conditions is also confirmed in tomato seedlings and maize seedlings [57,58]. Exogenous application of melatonin promotes plant growth and stress resistance mainly through enhancing the plant endogenous melatonin level.…”

Section: Discussionmentioning

confidence: 69%

Melatonin Application Improves Salt Tolerance of Alfalfa (Medicago sativa L.) by Enhancing Antioxidant Capacity

Cen

Wang

Liu

et al. 2020

Plants

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Alfalfa (Medicago sativa L.) is an important and widely cultivated forage grass. The productivity and forage quality of alfalfa are severely affected by salt stress. Melatonin is a bioactive molecule with versatile physiological functions and plays important roles in response to various biotic and abiotic stresses. Melatonin has been proven efficient in improving alfalfa drought and waterlogging tolerance in recent studies. In our reports, we applied melatonin exogenously to explore the effects of melatonin on alfalfa growth and salt resistance. The results demonstrated that melatonin application promoted alfalfa seed germination and seedling growth, and reduced oxidative damage under salt stress. Further application research found that melatonin alleviated salt injury in alfalfa plants under salt stress. The electrolyte leakage, malondialdehyde (MDA) content and H 2 O 2 content were significantly reduced, and the activities of catalase (CAT), peroxidase (POD), and Cu/Zn superoxide dismutase (Cu/Zn-SOD) were increased with melatonin pretreatment compared to control plants under salt stress with the upregulation of genes related to melatonin and antioxidant enzymes biosynthesis. Melatonin was also involved in reducing Na + accumulation in alfalfa plants. Our study indicates that melatonin plays a primary role as an antioxidant in scavenging H 2 O 2 and enhancing activities of antioxidant enzymes to improve the salt tolerance of alfalfa plants.Plants 2020, 9, 220 2 of 17 world's irrigated land affected by saline or sodic globally [7,8]. Salinity has become one of the most important environmental stress factors impairing worldwide agricultural productivity. Under salinity conditions, the seed germination, growth and development processes of alfalfa are inhibited and they finally impair the herbage yield, as well as the forage quality [9]. Breeding new alfalfa cultivars with high salt tolerance is always needed. Genetic engineering and conventional breeding have been proven efficient for improving salt tolerance of various plant species [10][11][12][13]. However, they are time-consuming and complicated. Exogenous application of certain plant growth regulators such as phytohormone and other small molecules has been proven efficient at overcoming the harmful effects of salt stress on plants [14][15][16][17]. Moreover, plant growth regulators in low concentrations always play a role in plants, and are cost-effective. Foliar spraying of salicylic acid (SA) on faba bean inhibits Na + accumulation and lipid peroxidation, improving the antioxidant resistance and finally mitigating the damage caused by salinity [18]. Exogenously applied poly-γ-glutamic acid on wheat maintains the Na + and K + homeostasis and enhances antioxidant capacity by alleviating salinity damage under salt stress conditions [19]. Exogenous spermidine application to salt-stressed cucumber improves the photosynthetic capacity and the activity of key enzymes for CO 2 fixation by regulating the expression of related genes, and tolerance to salinity is thus...

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“…Salt is one of the main factors affecting ecological environments and inhibiting crop growth. With the expansion of saline-alkali land areas in China, crop yields and quality have decreased gradually [34]. The salt stress damage to plants is mainly manifested through osmotic stress, ion stress, and antioxidant system and hormone signal transduction, among other phenomena [35,7].…”

Section: Discussionmentioning

confidence: 99%

“…Castanares et al [36] concluded that salt stress leads to K + / Na + imbalance, destroys cell membrane integrity, and reduces potassium retention, while exogenous melatonin effectively relieved this effect, which might be related to the activity of related enzymes in cells. Similarly, melatonin could significantly reduce Na + accumulation and increase K + content in maize seeds under salt stress [34]. Yu et al [51] proposed a novel mechanism for melatonin-mediated salt tolerance, i.e., melatonin supplementation decreased the oxidative damage induced by salinity, perhaps by directly scavenging H 2 O 2 or enhancing the activities of antioxidative enzymes.…”

Section: Discussionmentioning

confidence: 99%

Exogenous melatonin promotes seed germination and osmotic regulation under salt stress in cotton (Gossypium hirsutum L.)

et al. 2020

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Melatonin (MT; is an amine hormone involved in abiotic stress resistance. Previous studies have confirmed that melatonin can promote seed germination, mediate physiological regulation mechanisms, and stimulate crop growth under stress. However, the osmotic regulation mechanism by which exogenous melatonin mediates salt tolerance in cotton is still largely unknown. To investigate the effect of salt stress on melatonin concentration in germinating cotton seeds, we analyzed melatonin content over time during seed germination under different treatments. Melatonin content reached its minimum at day 6, while cotton germination rates peaked at day 6, indicating melatonin content and seed germination are correlated. Then we investigated the effects of 10-100 μM melatonin treatments on membrane lipid peroxides and osmotic adjustment substances during cotton seed germination under salt stress. Salt stress led to electrolyte leakage (EL) as well as accumulations of hydrogen peroxide (H 2 O 2 ), malondialdehyde (MDA), organic osmotic substances (i.e., proline, soluble sugars), and inorganic osmotic substances (i.e., Na + , Cl -). Meanwhile, the contents of melatonin, soluble proteins, and K + as well as the K + /Na + balance decreased, indicating that salt stress inhibited melatonin synthesis and damaged cellular membranes, seriously affecting seed germination. However, melatonin pretreatment at different concentrations alleviated the adverse effects of salt stress on cotton seeds and reduced EL as well as the contents of H 2 O 2 , MDA, Na + , and Cl -. The exogenous application of melatonin also promoted melatonin, soluble sugar, soluble proteins, proline, and K + /Na + contents under salt stress. These results demonstrate that supplemental melatonin can effectively ameliorate the repression of cotton seed germination by enhancing osmotic regulating substances and adjusting ion homeostasis under salt stress. Thus, melatonin may potentially be used to protect cotton seeds from salt stress, with the 20 μM melatonin treatment most effectively promoting cotton seed germination and improving salt stress tolerance. et al. (2020) Exogenous melatonin promotes seed germination and osmotic regulation under salt stress in cotton (Gossypium hirsutum L.). PLoS ONE 15(1): e0228241. https://doi.org/10.

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Exogenous melatonin enhances salt stress tolerance in maize seedlings by improving antioxidant and photosynthetic capacity

Cited by 220 publications

References 76 publications

Interactive Effect of Melatonin and UV-C on Phenylpropanoid Metabolite Production and Antioxidant Potential in Callus Cultures of Purple Basil (Ocimum basilicum L. var purpurascens)

Interactive Effect of Melatonin and UV-C on Phenylpropanoid Metabolite Production and Antioxidant Potential in Callus Cultures of Purple Basil (Ocimum basilicum L. var purpurascens)

Melatonin Application Improves Salt Tolerance of Alfalfa (Medicago sativa L.) by Enhancing Antioxidant Capacity

Exogenous melatonin promotes seed germination and osmotic regulation under salt stress in cotton (Gossypium hirsutum L.)

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