Exogenous malic acid alleviates cadmium toxicity in Miscanthus sacchariflorus through enhancing photosynthetic capacity and restraining ROS accumulation

Guo, Haipeng; Chen, Houming; Hong, Chuntao; Jiang, Dongxiang; Zheng, Bingsong

doi:10.1016/j.ecoenv.2017.03.018

Cited by 64 publications

(29 citation statements)

References 49 publications

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“…The POD activity in the roots of S. variegata increased significantly under the respective addition of three organic acids, and leaf POD activity also greatly increased after the malic acid addition. The increase of these enzymatic activities may be attributed to the enhanced expression of relevant antioxidative enzyme genes such as POD1, Cu/Zn-SOD, and GPX1 induced by organic acids (Guo et al, 2017). This also indicates that organic acid supply enhances the resistance of S. variegata to Cd-caused oxidative stress.…”

Section: Discussionmentioning

confidence: 95%

“…(3) Cd + citric acid group, 50 µM of Cd and 100 µM of citric acid; (4) Cd + tartaric acid group, 50 µM of Cd and 100 µM of tartaric acid; and (5) Cd+ malic acid group, 50 µM of Cd and 100 µM of malic acid. The concentrations of Cd and organic acids were chosen based on the typical content in the natural environment and the results of previous studies (Chen et al, 2020;Guo et al, 2017). All treatments contained three replicates.…”

Section: Treatment Testmentioning

confidence: 99%

“…Moreover, studies have shown that organic acids also play an important role in tricarboxylic acid cycle metabolism, photosynthetic and respiration, nutrient uptake, and toxic metal detoxification in plants (Schulze et al, 2002;Araujo et al, 2012). Under Cd stress, the addition of malic acid can alleviate Cd-induced oxidative damage by regulating the enzymatic and non-enzymatic antioxidants in Miscanthus sacchariflorus (Guo et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Effects of Exogenous Organic Acids on Cd Tolerance Mechanism of Salix variegata Franch. Under Cd Stress

Zhang

Chen

et al. 2020

Front. Plant Sci.

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Chelate induction of organic acids has been recognized to enhance metal uptake and translocation in plants, but the underlying mechanism remains unclear. In this study, seedlings of Salix variegata were hydroponically exposed to the combinations of Cd (0 and 50 µM) and three exogenous organic acids (100 µM of citric, tartaric, or malic acid). Plant biomass, antioxidant enzymes, non-protein thiol compounds (NPT) content, and the expression of candidate genes associated with Cd accumulation and tolerance were determined. Results showed that Cd significantly inhibited plant biomass but stimulated the activity of antioxidant enzymes in the roots and leaves, while the lipid peroxidation increased as well. Respective addition of three organic acids greatly enhanced plant resistance to oxidative stress and reduced the lipid peroxidation induced by Cd, with the effect of malic acid showing greatest. The addition of organic acids also significantly increased the content of glutathione in the root, further improving the antioxidant capacity and potential of phytochelatin biosynthesis. Moreover, Cd induced the expression level of candidate genes in roots of S. variegata. The addition of three organic acids not only promoted the expression of candidate genes but also drastically increased Cd accumulation in S. variegata. In summary, application of citric, tartaric, or malic acid alleviated Cd-imposed toxicity through the boost of enzymatic and non-enzymatic antioxidants and candidate gene expression, while their effects on Cd tolerance and accumulation of S. variegata differed.

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Section: Discussionmentioning

confidence: 95%

Section: Treatment Testmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Exogenous Organic Acids on Cd Tolerance Mechanism of Salix variegata Franch. Under Cd Stress

Zhang

Chen

et al. 2020

Front. Plant Sci.

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“…Therefore, the production of shorter roots in C. olitorius plants treated with 15 mg/kg Cd probably indicates the limit of Cd tolerance for this species. Application of 100 µM Cd also retarded the length of Miscanthus sacchariflorus roots [ 31 ]. In Paspalum fasciculatum grown in 15–50 mg/kg Cd contaminated soil, root biomass increased at 30 mg/kg, whereas stem and leaf biomass of all treated plants was similar to the control, at 30 days after sowing [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Response of Corchorus olitorius Leafy Vegetable to Cadmium in the Soil

Ndlovu

Pullabhotla

Ntuli

2020

Plants

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Corchorus olitorius, a leafy vegetable with high nutrient content, is normally collected from the wild, in areas that are prone to cadmium (Cd) toxicity. However, studies on how Cd accumulation affects vegetative and reproductive traits of leafy vegetables in South Africa are limited. Therefore, this study tested the effect of Cd accumulation on C. olitorius morphological traits. Plants were grown under various Cd concentrations and studied for variation in vegetative and reproductive traits as well as accumulation in roots and shoots. Plants exposed to 5 mg/kg Cd had longer roots with higher moisture content, heavier fresh and dried stems, as well as dried leaves, which indicated a hormetic effect in C. olitorius after exposure to low Cd concentration in the soil. Again, plants treated with 5–10 mg/kg Cd, accumulated toxic (>10 mg/kg dry weight) Cd within shoots and roots, with minor morphological alterations. Plants could survive, with some morphological defects, Cd toxicity up to 20 mg/kg in soil. Only plants exposed to 5 mg/kg could reproduce. Cd accumulation increased with an increase in the soil, with higher accumulation in shoots. The translocation factor was high (>1) in all Cd concentrations. In conclusion, C. olitorius can accumulate toxic Cd, and yet grow and reproduce either normally or better than the control. The proposed dose of Cd that induces hormesis in C. olitorius is 5 mg/kg in the soil. Therefore, C. olitorius is suitable for phytoremediation of Cd contaminated soils, but unsafe for consumption when it grows in such areas.

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“…slr0721 encodes the decarboxylating NADP-dependent malic enzyme, participating in tricarboxylic acid cycle. A study on malic acid and Cd 2+ stress in Miscanthus sacchariflorus proved exogenous addition of malic acid could alleviate Cd 2+ toxicity through enhancing photosynthetic capacity and restraining ROS accumulation [ 41 ]. Even though this phenomenon was widely discovered in plants [ 42 , 43 ], we supposed that similar tolerance mechanism might also be utilized in cyanobacteria, while further evidences are still needed.…”

Section: Discussionmentioning

confidence: 99%

Adaptive laboratory evolution of cadmium tolerance in Synechocystis sp. PCC 6803

Sun

et al. 2018

Biotechnol Biofuels

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BackgroundCadmium has been a significant threat to environment and human health due to its high toxicity and wide application in fossil-fuel burning and battery industry. Cyanobacteria are one of the most dominant prokaryotes, and the previous studies suggested that they could be valuable in removing Cd2+ from waste water. However, currently, the tolerance to cadmium is very low in cyanobacteria. To further engineer cyanobacteria for the environmental application, it is thus necessary to determine the mechanism that they respond to high concentration of cadmium.ResultsIn this study, a robust strain of Synechocystis PCC 6803 (named ALE-9.0) tolerant to CdSO4 with a concentration up to 9.0 µM was successfully isolated via adaptive laboratory evolution over 802-day continuous passages under cadmium stress. Whole-genome re-sequencing was then performed and nine mutations were identified for the evolved strain compared to the wild-type strain. Among these mutations, a large fragment deletion in slr0454 encoding a cation or drug efflux system protein was found to contribute directly to the resistance to Cd2+ stress. In addition, five other mutations were also demonstrated related to the improved Cd2+ tolerance in ALE-9.0. Moreover, the evolved ALE-9.0 strain was found to obtain cross tolerance to some other heavy metals like zinc and cobalt as well as higher resistance to high light.ConclusionsThe work here identified six genes and their mutations related to Cd2+ tolerance in Synechocystis PCC 6803, and demonstrated the feasibility of adaptive laboratory evolution in tolerance modifications. This work also provided valuable information regarding the cadmium tolerance mechanism in Synechocystis PCC 6803, and useful insights for cyanobacterial robustness and tolerance engineering.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1205-x) contains supplementary material, which is available to authorized users.

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Exogenous malic acid alleviates cadmium toxicity in Miscanthus sacchariflorus through enhancing photosynthetic capacity and restraining ROS accumulation

Cited by 64 publications

References 49 publications

Effects of Exogenous Organic Acids on Cd Tolerance Mechanism of Salix variegata Franch. Under Cd Stress

Effects of Exogenous Organic Acids on Cd Tolerance Mechanism of Salix variegata Franch. Under Cd Stress

Response of Corchorus olitorius Leafy Vegetable to Cadmium in the Soil

Adaptive laboratory evolution of cadmium tolerance in Synechocystis sp. PCC 6803

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