Exogenous insulin promotes the expression of B-cell translocation gene 1 and 2 in chicken pectoralis

Zhao, Jing; Zhang, X.L.; Gao, Linge; Guo, Yujie; Wang, Z.Y.; Su, Chuanchen; Zhang, Huaiyong; Chen, W.; Huang, Yanqun

doi:10.1016/j.psj.2022.101875

“…The results obtained show that the level of glucose in T1 was not within the normal range for local chickens, which is between (200-450 mg / 100 ml) of blood plasma, and the explanation for this is that glucose is affected by the environmental, nutritional and health status of birds (Zhao et al, 2022), the reason for the rise in glucose sugar above the normal range in T1 may be due to the stress the birds are exposed to, which works to liberate the hormone corticosterone from the adrenal cortex (A.R. Alkhateeb et al, 2022), as this hormone works to supply glucose sugar to the body from noncarbohydrate sources in a qualitative process known as (gluconeogenesis)…”

Section: Resultsmentioning

confidence: 76%

Impact of Habituation and Gene Silencing on Blood Biochemical Indicators and Welfare in Local Chickens

Mahmood,

Abdulateef,

Alnori

et al. 2024

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Chickens respond to the signals of other chickens, and as the signals are repeated, the chicken's reactions adapt over time. In studies of chickens responding to repeated playbacks of recorded chicken signals, the word "habituation" has been used to describe both overt behaviours and behavioural responses during specific tests in both field and laboratory settings. Gene silencing, on the other hand, is the regulation of gene expression in a cell that inhibits a particular gene from expressing. The current study aimed to reduce the stress responses of a breed of local chickens by changing their behavioural or genetic factors, and assessed blood traits that reflect their level of welfare. The study used 120 hen chickens obtained at 22 weeks old, divided into six treatments, with four replicates for each treatment (20 chicks per treatment, 5 chicks per replicate) as follows: T1 was a negative control treatment consisting of a local Iraqi chicken breed; T2 was a positive control treatment consisting of a commercial breed of chickens; T3 involved the local breed with siRNA used to silence the corticosterone hormone pathway to the CYP11B2 and CYP11B1 genes by injection in the jugular vein at the age of 24 and 40 weeks; T4 consisted of mixed groups with 25% commercial chicken + 75% local chicks; whilst T5 consisted of 50% commercial chicken + 50% local chicken; and T6 consisted of 75% commercial chicken + 25% local chicken. The results showed a significant improvement (P≤0.05) in Glucose, Cholesterol, Total Protein, LDL, HDL, Globulin and Albumin for T3 and T5 compared with the other treatments; and a significant improvement (P≤0.05) in blood corticosterone in T3 immediately after the injection and two weeks later. The results show that there is an effect of habituation and gene silencing on blood traits, indicating an improved level of welfare for the T3 and T5 chickens.

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“…The results obtained show that the level of glucose in T1, the control group of local wild-type chickens, was not within the normal range for local chickens, which is between (200-450 mg / 100 ml) of blood plasma. The explanation for this is that glucose is affected by the environmental, nutritional, and health status of the birds (Zhao et al, 2022). The reason for the rise in glucose levels above the normal range in T1 may be due to the stress the birds were exposed to, which works to release the hormone corticosterone from the adrenal cortex (A.R.…”

Section: Resultsmentioning

confidence: 99%

Effect of habituation and gene silencing on blood biochemical traits in local chicken indicative of their level of welfare

Mahmood,

Abdulateef,

Alnori

et al. 2024

Preprint

0

View full text Add to dashboard Cite

Chickens respond to the signals of other chickens, and as the signals are repeated, the chicken's reactions adapt over time. In studies of chickens responding to repeated playbacks of recorded chicken signals, the word "habituation" has been used to describe both overt behaviours and behavioural responses during specific tests in both field and laboratory settings. Gene silencing, on the other hand, is the regulation of gene expression in a cell that inhibits a particular gene from expressing. The current study aimed to reduce the stress responses of a breed of local chickens by changing their behavioural or genetic factors, and assessed blood traits that reflect their level of welfare. The study used 120 hen chickens obtained at 22 weeks old, divided into six treatments, with four replicates for each treatment (20 chicks per treatment, 5 chicks per replicate) as follows: T1 was a negative control treatment consisting of a local Iraqi chicken breed; T2 was a positive control treatment consisting of a commercial breed of chickens; T3 involved the local breed with siRNA used to silence the corticosterone hormone pathway to the CYP11B2 and CYP11B1 genes by injection in the jugular vein at the age of 24 and 40 weeks; T4 consisted of mixed groups with 25% commercial chicken + 75% local chicks; whilst T5 consisted of 50% commercial chicken + 50% local chicken; and T6 consisted of 75% commercial chicken + 25% local chicken. The results showed a significant improvement (P≤0.05) in Glucose, Cholesterol, Total Protein, LDL, HDL, Globulin and Albumin for T3 and T5 compared with the other treatments; and a significant improvement (P≤0.05) in blood corticosterone in T3 immediately after the injection and two weeks later. The results show that there is an effect of habituation and gene silencing on blood traits, indicating an improved level of welfare for the T3 and T5 chickens.

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“…It was proven that BTG1 and BTG2 are abundantly expressed in skeletal muscles. There were also differences between broilers and laying hens in gene expression levels in the sternum, pancreas, and heart ( Zhao et al, 2022 ). It can be assumed that when analyzing the nucleotide sequence of these genes, differences in polymorphic sites can be determined in meat- and egg-producing hens, which would indicate a direct relationship with productivity and the prospects of these genes as markers affecting metabolism.…”

Section: Effects Of Btg1 and Btg2 Genesmentioning

confidence: 99%

“…The level of expression of BTG1 and BTG2 genes in chickens depends on the breeds and type of the tissue. So the expression of BTG genes in thoracic and pancreatic cells was negatively correlated with the glucose level in the bloodstream of chickens ( Zhao et al, 2022 ).…”

Section: Relationship Between Insulin and Btg1 And Btg2 Genesmentioning

confidence: 99%

The role of BTG1 and BTG2 genes and their effects on insulin in poultry

Kulikov,

Malakheeva,

Komarchev

2024

Front. Physiol.

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Exogenous insulin promotes the expression of B-cell translocation gene 1 and 2 in chicken pectoralis

Cited by 4 publications

References 28 publications

Impact of Habituation and Gene Silencing on Blood Biochemical Indicators and Welfare in Local Chickens

Impact of Habituation and Gene Silencing on Blood Biochemical Indicators and Welfare in Local Chickens

Effect of habituation and gene silencing on blood biochemical traits in local chicken indicative of their level of welfare

The role of BTG1 and BTG2 genes and their effects on insulin in poultry

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