Exogenous inoculation of endophytic bacterium Bacillus cereus suppresses clubroot (Plasmodiophora brassicae) occurrence in pak choi (Brassica campestris sp. chinensis L.)

Arif, Samiah; Liaquat, Fiza; Yang, Shuofei; Shah, Iftikhar Hussain; Zhao, Lina; Xiong, Xue; García, Daniel; Zhang, Yidong

doi:10.1007/s00425-020-03546-4

Cited by 18 publications

(12 citation statements)

References 71 publications

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“…Li et al (2018) suggesting that EC could be a new tool for monitoring soil-borne disease. Arif et al (2021) found that the relative electrical conductivity of roots inoculated with P. brassicae was 2.85 times greater than that of the control, which may be due to the increased membrane permeability and cell membrane damage. In our study, the F-1000 treatment (with the least disease) showed a lower EC value than the other inoculated treatments (Table S2).…”

Section: Ec Was Positively Correlated With DI Of Clubroot Disease and The Correlation Was The Highest Among All Soil Propertiesmentioning

confidence: 90%

Comparison of the effects of three fungicides on clubroot disease of tumorous stem mustard and soil bacterial community

et al. 2021

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Purpose The application of fungicides is one of the main strategies to prevent clubroot disease. Currently, numerous studies focus on changes in the soil microbial community at different levels of clubroot disease severity. However, the effects of fungicides on the soil microbial community and causative pathogen, Plasmodiophora brassicae, while preventing clubroot disease remain unclear. Methods In this study, we evaluated the control efficacy of three fungicides (fluazinam, metalaxyl-mancozeb, and carbendazim) on clubroot disease of tumorous stem mustard in greenhouse experiment. Uninoculated and Water treatments after inoculation were performed as controls. At three (3 W) and six weeks (6 W) post-inoculation of P. brassicae, soil properties, bacterial composition (sequencing of 16S rRNA genes), and effector gene expression of the pathogen were analyzed. The correlation of these factors with disease index (DI) was explored. Results Fluazinam was the most effective in controlling clubroot disease of tumorous stem mustard with a controlled efficacy of 59.81%, and the abundance of P. brassicae in the soil decreased 21.29% after 3 weeks of treatment. Compared with other treatments, twelve out of twenty effector genes showed higher expression in fluazinam 3 W samples. Different fungicides had different effects on soil properties. EC (electrical conductivity), the main factor that positively associated with DI, was significantly lower in fluazinam treatment than the other two fungicide treatments. The application of fungicides, especially carbendazim, significantly reduced bacterial α-diversity and the composition of soil bacteria. Pseudomonas, Microbacterium, and Sphingobacterium (positively correlated with DI) were enriched in Water, metalaxyl-mancozeb, and carbendazim treatments, but were less abundant in fluazinam treatment. Among the three fungicide treatments, DI was significantly negatively correlated with Shannon and Chao 1 indices. Soil properties and the top bacterial genera that positively correlated with DI were influenced to a lesser degree in the fluazinam treatment. Conclusion Among three fungicides, fluazinam was the most effective agent with the highest control effects against clubroot disease. The strong virulence of fluazinam against P. brassicae was one of the main reasons for the prevention of clubroot disease, and in addition the alteration of rhizosphere bacterial community by fluazinam to the detriment of P. brassicae infection. Based on our results, EC could be an indicator of the severity of clubroot disease.

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Section: Ec Was Positively Correlated With DI Of Clubroot Disease and The Correlation Was The Highest Among All Soil Propertiesmentioning

confidence: 90%

Comparison of the effects of three fungicides on clubroot disease of tumorous stem mustard and soil bacterial community

et al. 2021

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“…(2019) revealed that the application of B. cereus in tomato plants was able to colonise and inhibit the growth of bacterial wilt pathogen, Ralstonia solanacearum . A recent study by Arif et al . (2021) found that B. cereus applied in Brassica campestris could colonise and inhibit the growth of clubroot pathogen.…”

Section: Resultsmentioning

confidence: 97%

Bacillus cereus for Controlling Bacterial Heart Rot in Pineapple var. MD2

Husin¹,

Sapak²

2022

tlsr

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Bacterial heart rot (BHR) disease caused by pathogenic bacteria, Dickeya zeae, is one of the destructive diseases of pineapple worldwide. This study explored the potential of Bacillus cereus against the BHR pathogen in vitro and in vivo. The BHR causal pathogen was isolated from symptomatic pineapple plants, demonstrating watersoaked and rotten basal tissues. Biological control agent (BCA) was isolated from asymptomatic pineapple leaves, later confirmed as B. cereus, and subsequently tested for the antagonistic activity against the BHR pathogen via disc diffusion assay and glasshouse trial. B. cereus showed the ability to inhibit the growth of BHR pathogen with 18.10 ± 0.36 mm of inhibition zone in diameter. The ability of B. cereus against the BHR pathogen was further confirmed via the glasshouse trial with five treatments. The results showed that treatments with B. cereus inoculation recorded lower disease severity index of 0.04 ± 0.01 than the positive control treatment with pathogen alone (0.53 ± 0.04). This finding indicated that B. cereus has a great potential as BCA against BHR disease in pineapple var. MD2, however, the effectiveness of this isolate needs to be further tested under actual field conditions.

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“…This protein shows notable similarity in its C-terminal region (50% identity over 16 amino acids and E value of 8.5) to silacidin A, a silica-forming peptide that is isolated from cell walls of the diatom Thalassiosira pseudonana ( 51 ). Moreover, B. cereus genome contains a silicon transporter gene with some similarity with the higher plant Lsi2 sequences ( 3 , 52 ). By contrast, no gene homolog to the ones known to be involved in silica deposition has yet been detected in the cyanobacterium Synechococcus ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Intracellular silicification by early-branching magnetotactic bacteria

Liu

Menguy

et al. 2022

Sci. Adv.

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Biosilicification—the formation of biological structures composed of silica—has a wide distribution among eukaryotes; it plays a major role in global biogeochemical cycles, and has driven the decline of dissolved silicon in the oceans through geological time. While it has long been thought that eukaryotes are the only organisms appreciably affecting the biogeochemical cycling of Si, the recent discoveries of silica transporter genes and marked silicon accumulation in bacteria suggest that prokaryotes may play an underappreciated role in the Si cycle, particularly in ancient times. Here, we report a previously unidentified magnetotactic bacterium that forms intracellular, amorphous silica globules. This bacterium, phylogenetically affiliated with the phylum Nitrospirota, belongs to a deep-branching group of magnetotactic bacteria that also forms intracellular magnetite magnetosomes and sulfur inclusions. This contribution reveals intracellularly controlled silicification within prokaryotes and suggests a previously unrecognized influence on the biogeochemical Si cycle that was operational during early Earth history.

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Exogenous inoculation of endophytic bacterium Bacillus cereus suppresses clubroot (Plasmodiophora brassicae) occurrence in pak choi (Brassica campestris sp. chinensis L.)

Cited by 18 publications

References 71 publications

Comparison of the effects of three fungicides on clubroot disease of tumorous stem mustard and soil bacterial community

Comparison of the effects of three fungicides on clubroot disease of tumorous stem mustard and soil bacterial community

Bacillus cereus for Controlling Bacterial Heart Rot in Pineapple var. MD2

Intracellular silicification by early-branching magnetotactic bacteria

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