Exogenous Hsp70 attenuates nitroglycerin-induced migraine-like symptoms in mice

Wu, Fengfang; Huang, Yifeng; Wei, Guifang; Huang, Zhiwei; Shi, Congwen; Lin, Weinian; Liao, Jun

doi:10.1152/jn.00314.2021

Cited by 4 publications

(2 citation statements)

References 30 publications

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“…Our RNA sequencing data showed that while this gene was expressed in both sexes, it was specifically upregulated only in males post tumor growth. Interestingly, hsp70 has been demonstrated to have a protective role in pain during nerve damage 111 , 112 , migraine 113 and opioid-induced hyperalgesia 114 .Therefore, upregulation of Hsp70 in males post-tumor growth might indicate that male neurons might express an endogenous feedback mechanism to suppress pain that may be lacking in females.…”

Section: Discussionmentioning

confidence: 99%

Sex-dependent differences in the genomic profile of lingual sensory neurons in naïve and tongue-tumor bearing mice

Ibrahim,

Wu,

Wang

et al. 2023

Sci Rep

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Mechanisms of sex-dependent orofacial pain are widely understudied. A significant gap in knowledge exists about comprehensive regulation of tissue-specific trigeminal sensory neurons in diseased state of both sexes. Using RNA sequencing of FACS sorted retro-labeled sensory neurons innervating tongue tissue, we determined changes in transcriptomic profiles in males and female mice under naïve as well as tongue-tumor bearing conditions Our data revealed the following interesting findings: (1) FACS sorting obtained higher number of neurons from female trigeminal ganglia (TG) compared to males; (2) Naïve female neurons innervating the tongue expressed immune cell markers such as Csf1R, C1qa and others, that weren’t expressed in males. This was validated by Immunohistochemistry. (3) Accordingly, immune cell markers such as Csf1 exclusively sensitized TRPV1 responses in female TG neurons. (4) Male neurons were more tightly regulated than female neurons upon tumor growth and very few differentially expressed genes (DEGs) overlapped between the sexes, (5) Male DEGs contained higher number of transcription factors whereas female DEGs contained higher number of enzymes, cytokines and chemokines. Collectively, this is the first study to characterize the effect of sex as well as of tongue-tumor on global gene expression, pathways and molecular function of tongue-innervating sensory neurons.

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Section: Discussionmentioning

confidence: 99%

Sex-dependent differences in the genomic profile of lingual sensory neurons in naïve and tongue-tumor bearing mice

Ibrahim,

Wu,

Wang

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Our RNA sequencing data showed that while this gene was expressed in both sexes, it was specifically upregulated only in males post tumor growth. Interestingly, hsp70 has been demonstrated to have a protective role in pain during nerve damage (99,100), migraine (101) and opioid-induced hyperalgesia (102).Therefore, upregulation of Hsp70 in males post-tumor growth might indicate that male neurons might express an endogenous feedback mechanism to suppress pain that may be lacking in females. In addition to the common BPs, sex-selective BPs were identified post-tumor growth.…”

Section: Discussionmentioning

confidence: 99%

Sex-dependent Differences in the Genomic Profile of Lingual Sensory Neurons in Naïve and Tongue-Tumor Bearing Mice

Ibrahim

Wang

et al. 2023

Preprint

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While sex-specific prevalence of orofacial pain is established, mechanisms of sex-dependent orofacial pain are widely understudied. To this end, a significant gap in knowledge exists about comprehensive regulation of tissue-specific trigeminal sensory neurons in diseased state of males and females. Using RNA sequencing of FACS sorted retro-labeled sensory neurons innervating tongue tissue, we determined changes in transcriptomic profiles in males and female mice under naive as well as tongue-tumor bearing conditions Our data revealed the following interesting findings: 1) Tongue tissue of female mice was innervated with higher number of trigeminal neurons compared to males; 2) Naive female neurons innervating the tongue exclusively expressed immune cell markers such as Csf1R, C1qa and others, that werent expressed in males. This was validated by Immunohistochemistry. 3) Male neurons were more tightly regulated than female neurons upon tumor growth; 4) While very few differentially expressed genes (DEGs) overlapped between males and females post-tumor growth, several biological processes (BPs) were similar between two sexes. However, additional distinct processes were sex-specific; 5) Post-tumor growth, male DEGs contained an equal mix of transcription factors, ligands, growth factors, receptors and channels, whereas female DEGs predominantly contained channels/receptors, enzymes, cytokines and chemokines. Taken together, this is the first study to characterize the effect of sex as well as of tongue-tumor on global gene expression, biological pathways and molecular function of tongue-innervating sensory neurons.

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