Exogenous GABA application transiently improves the tolerance to root hypoxia on a sensitive genotype of Prunus rootstock

Salvatierra, Ariel; Pimentel, Paula; Almada, Rubén; Hinrichsen, Patricio

doi:10.1016/j.envexpbot.2016.01.009

Cited by 49 publications

(31 citation statements)

References 107 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, stomatal closure has been found to be responsible for A CO2 reduction in hypoxic conditions (Syvertsen et al , Mielke et al , Islam and Macdonald , Nicolás et al ), which would explain the reductions in photosynthesis observed here in sweet cherry. This decline in photosynthesis due to stomatal closure has been described previously in Prunus species submitted to W (Beckman et al , Domingo et al , Nicolás et al , Insausti and Gorjón , Pimentel et al , Salvatierra et al ). By contrast, when Pimentel et al () studied the W tolerance of ‘Mariana 2624’ after 7 and 14 days of flooding, they discovered that gs and A CO2 were maintained as in control conditions.…”

Section: Discussionsupporting

confidence: 76%

Two minuses can make a plus: waterlogging and elevated CO₂ interactions in sweet cherry (Prunus avium) cultivars

Pérez‐Jiménez

Hernández-Munuera

Piñero

et al. 2017

Physiologia Plantarum

View full text Add to dashboard Cite

The increase in the ambient concentration of CO and other greenhouse gases is producing climate events that can compromise crop survival. However, high CO concentrations are sometimes able to mitigate certain stresses such as salinity or drought. In this experiment, the effects of waterlogging and CO are studied in combination to elucidate the eventual response in sweet cherry trees. For this purpose, four sweet cherry cultivars ('Burlat', 'Cashmere', 'Lapins and 'New Star') were grafted on a typically hypoxia-tolerant rootstock (Mariana 2624) and submitted to waterlogging for 7 days at either ambient CO concentration (400 µmol mol ) or at elevated CO (800 µmol mol ). Waterlogging affected plants drastically, by decreasing photosynthesis, stomatal conductance, transpiration, chlorophyll fluorescence and growth. It also brought about the accumulation of proline, chloride and sulfate. Nonetheless, raising the CO supply not only mitigated all these effects but also induced the accumulation of soluble sugars and starch in the leaf. Therefore, sweet cherry plants submitted to waterlogging were able to overcome this stress when grown in a CO -enriched environment.

show abstract

Section: Discussionsupporting

confidence: 76%

Two minuses can make a plus: waterlogging and elevated CO₂ interactions in sweet cherry (Prunus avium) cultivars

Pérez‐Jiménez

Hernández-Munuera

Piñero

et al. 2017

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

“…In the current study, SlGAD1-3 were upregulated, but SlGAD4 was downregulated, mainly because different isoforms have different functions under stress. In previous work, exogenous GABA improved PSGAD2 and PSGAD4 transcription levels compared with those under single hypoxia treatment [41]. Our results showed that the relative expression of SlGAD1-3 in the Na+G-treatment group was signi cantly higher than that in the Na-treatment group.…”

Section: Discussionsupporting

confidence: 55%

Gamma-aminobutyric acid (GABA) alleviates salt damage in tomato by modulating Na+ uptake, the GAD gene, amino acid synthesis and reactive oxygen species metabolism

Jia

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Salt stress is a serious abiotic stress that caused crop growth inhibition and yield decline. Previous studies have reported on the the synthesis of gamma-aminobutyric acid (GABA) and its relationship with plant resistance under various abiotic stress. However, the relationship between exogenous GABA alleviating plant salt stress damage and ion flux, amino acid synthesis, and key enzyme expression remains largely unclear. We investigated plant growth, Na+ transportation and accumulation, reactive oxygen species (ROS) metabolism and evaluated the effect of GABA on amino acids especially SlGADs gene expression and the endogenous GABA content of tomato (Solanum lycopersicum L.) seedlings treated with or without 5 mmol·L-1 GABA under 175 mmol·L-1 NaCl stress. Results: Exogenous application of GABA significantly reduced the salt damage index and increased plant height, chlorophyll content and the dry and fresh weights of tomato plants exposed to NaCl stress. GABA significantly reduced Na+ accumulation in leaves and roots by preventing Na+ influx in roots and transportation to leaves. The transcriptional expression of SlGAD1-3 genes were induced by NaCl stress especially with GABA application. Among them, SlGAD1 expression was the most sensitive and contributed the most to the increase in glutamic acid decarboxylase (GAD) activity induced by NaCl and GABA application; Exogenous GABA increased GAD activity and amino acid contents in tomato leaves compared with the levels under NaCl stress alone, especially the levels of endogenous GABA, proline, glutamate and eight other amino acids. These results indicated that SlGADs transcriptional expression played an important role in tomato plant resistance to NaCl stress with GABA application by enhancing GAD activity and amino acid content. GABA significantly alleviated the active oxygen-related injury of leaves under NaCl stress by increasing the activities of antioxidant enzymes and decreasing the contents of active oxygen species and malondialdehyde.Conclusion: Exogenous GABA had a positive effect on the resistance of tomato seedlings to salt stress, which was closely associated with reducing Na+ flux from root to leaves, increasing amino acid content and strengthening antioxidant metabolism. Endogenous GABA content was induced by salt and exogenous GABA at both the transcriptional and metabolic levels.

show abstract

“…Under hypoxia stress, it has been observed that the gas exchange parameters are dramatically affected in several fruit trees, such as avocado ( Persea americana Mill.) [ 33 ], kiwi fruits ( Actinidia chinensis Planch) [ 34 ], citrus trees [ 35 , 36 , 37 , 38 ], pecans ( Carya illinoensis K. Koch) [ 39 ], walnut trees ( Juglans regia L.) [ 40 ], grapevine ( Vitis vinifera L.) [ 41 , 42 ], pomegranate ( Punica granatum L.) [ 43 ], apple ( Malus × domestica Borkh) [ 44 ] and several Prunus species [ 7 , 45 , 46 , 47 , 48 , 49 ]. In general, all these tree species were classified as sensitive to root hypoxia.…”

Section: Fruit Tree Responses To O 2 Deficiencymentioning

confidence: 99%

“…In Prunus rootstocks under long-term hypoxia, ‘Mariana 2624’ plants, the hypoxia-tolerant genotype, showed no significant changes in MDA concentration in roots and leaves in comparison with the control plants. Opposingly, the sensitive rootstock ‘Mazzard F12/1’ showed a higher MDA concentration in roots and leaves after seven days of waterlogging, suggesting less capacity to remove ROS than the tolerant genotype [ 7 , 47 ]. Along with a lower MDA content in the roots of the hypoxia-tolerant genotype, a lower electrolyte leakage rate was also observed.…”

Section: Fruit Tree Responses To O 2 Deficiencymentioning

confidence: 99%

“…PDC and ALCOHOL DEHYDROGENASE (ADH) transcripts were found to be expressed in each transcriptome analyzed. Both PDC and ADH transcripts were coordinately induced in ‘Mariana 2624’ and ‘Mazzard F12/1’ under O 2 deficiency, although the hypoxia-tolerant genotype showed a decreasing trend after 6 h of waterlogging ( Figure 1 ) [ 47 ]. This transcriptional finding suggests that ‘Mariana 2624’ resorts to other adaptation mechanisms, distinct to the fermentation pathways, after the first hours of flooding.…”

Section: Transcriptomic Reprogramming Of Principal Pathways Involvmentioning

confidence: 99%

See 1 more Smart Citation

Keep Calm and Survive: Adaptation Strategies to Energy Crisis in Fruit Trees under Root Hypoxia

Salvatierra¹,

Toro²,

Mateluna³

et al. 2020

Plants

Self Cite

View full text Add to dashboard Cite

Plants are permanently facing challenges imposed by the environment which, in the context of the current scenario of global climate change, implies a constant process of adaptation to survive and even, in the case of crops, at least maintain yield. O2 deficiency at the rhizosphere level, i.e., root hypoxia, is one of the factors with the greatest impact at whole-plant level. At cellular level, this O2 deficiency provokes a disturbance in the energy metabolism which has notable consequences on the yield of plant crops. In this sense, although several physiological studies describe processes involved in plant adaptation to root hypoxia in woody fruit trees, with emphasis on the negative impacts on photosynthetic rate, there are very few studies that include -omics strategies for specifically understanding these processes in the roots of such species. Through a de novo assembly approach, a comparative transcriptome study of waterlogged Prunus spp. genotypes contrasting in their tolerance to root hypoxia was revisited in order to gain a deeper insight into the reconfiguration of pivotal pathways involved in energy metabolism. This re-analysis describes the classically altered pathways seen in the roots of woody fruit trees under hypoxia, but also routes that link them to pathways involved with nitrogen assimilation and the maintenance of cytoplasmic pH and glycolytic flow. In addition, the effects of root hypoxia on the transcription of genes related to the mitochondrial oxidative phosphorylation system, responsible for providing adenosine triphosphate (ATP) to the cell, are discussed in terms of their roles in the energy balance, reactive oxygen species (ROS) metabolism and aerenchyma formation. This review compiles key findings that help to explain the trait of tolerance to root hypoxia in woody fruit species, giving special attention to their strategies for managing the energy crisis. Finally, research challenges addressing less-explored topics in recovery and stress memory in woody fruit trees are pointed out.

show abstract

Exogenous GABA application transiently improves the tolerance to root hypoxia on a sensitive genotype of Prunus rootstock

Cited by 49 publications

References 107 publications

Two minuses can make a plus: waterlogging and elevated CO₂ interactions in sweet cherry (Prunus avium) cultivars

Two minuses can make a plus: waterlogging and elevated CO₂ interactions in sweet cherry (Prunus avium) cultivars

Gamma-aminobutyric acid (GABA) alleviates salt damage in tomato by modulating Na+ uptake, the GAD gene, amino acid synthesis and reactive oxygen species metabolism

Keep Calm and Survive: Adaptation Strategies to Energy Crisis in Fruit Trees under Root Hypoxia

Contact Info

Product

Resources

About

Exogenous GABA application transiently improves the tolerance to root hypoxia on a sensitive genotype of Prunus rootstock

Cited by 49 publications

References 107 publications

Two minuses can make a plus: waterlogging and elevated CO2 interactions in sweet cherry (Prunus avium) cultivars

Two minuses can make a plus: waterlogging and elevated CO2 interactions in sweet cherry (Prunus avium) cultivars

Gamma-aminobutyric acid (GABA) alleviates salt damage in tomato by modulating Na+ uptake, the GAD gene, amino acid synthesis and reactive oxygen species metabolism

Keep Calm and Survive: Adaptation Strategies to Energy Crisis in Fruit Trees under Root Hypoxia

Contact Info

Product

Resources

About

Two minuses can make a plus: waterlogging and elevated CO₂ interactions in sweet cherry (Prunus avium) cultivars

Two minuses can make a plus: waterlogging and elevated CO₂ interactions in sweet cherry (Prunus avium) cultivars