Exogenous ethylene inhibits sprout growth in onion bulbs

Bufler, G.

doi:10.1093/aob/mcn203

Cited by 34 publications

(28 citation statements)

References 16 publications

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“…Comparison between onions stored in continuous air or ethylene revealed that those stored in ethylene had reduced sprout growth, which is consistent with similar previous work (Bufler, 2009). Reduced sprout growth in response to continuous ethylene storage was also supported by the microarray data.…”

Section: Sprout Suppression Through Exogenous Ethylene Down-regulatessupporting

confidence: 90%

“…Bufler (2009) found that Copra onions held in continuous ethylene (10.6 mL L 21 ) had reduced sprout growth compared with those held in air. Surprisingly, treatment with 1-MCP for 24 h after curing (6 weeks at 28°C prior to cold storage) reduced sprout growth in SuperSweet1 onions when stored at 4°C or 12°C (Chope et al, 2007a).…”

mentioning

confidence: 95%

“…Both ethylene and 1-methylcyclopropene (1-MCP) have been shown to inhibit sprout growth (Chope et al, 2007a;Bufler, 2009), which decreases bulb quality. Onion quality is dependent on the rate of internal sprout growth during storage.…”

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confidence: 99%

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Ethylene and 1-Methylcyclopropene Differentially Regulate Gene Expression during Onion Sprout Suppression

et al. 2011

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Onion (Allium cepa) is regarded as a nonclimacteric vegetable. In onions, however, ethylene can suppress sprouting while the ethylene-binding inhibitor 1-methylcyclopropene (1-MCP) can also suppress sprout growth; yet, it is unknown how ethylene and 1-MCP elicit the same response. In this study, onions were treated with 10 mL L 21 ethylene or 1 mL L 21 1-MCP individually or in combination for 24 h at 20°C before or after curing (6 weeks) at 20°C or 28°C and then stored at 1°C. Following curing, a subset of these same onions was stored separately under continuous air or ethylene (10 mL L 21 ) at 1°C. Onions treated with ethylene and 1-MCP in combination after curing for 24 h had reduced sprout growth as compared with the control 25 weeks after harvest. Sprout growth following storage beyond 25 weeks was only reduced through continuous ethylene treatment. This observation was supported by a higher proportion of down-regulated genes characterized as being involved in photosynthesis, measured using a newly developed onion microarray. Physiological and biochemical data suggested that ethylene was being perceived in the presence of 1-MCP, since sprout growth was reduced in onions treated with 1-MCP and ethylene applied in combination but not when applied individually. A cluster of probes representing transcripts up-regulated by 1-MCP alone but down-regulated by ethylene alone or in the presence of 1-MCP support this suggestion. Ethylene and 1-MCP both down-regulated a probe tentatively annotated as an ethylene receptor as well as ethylene-insensitive 3, suggesting that both treatments down-regulate the perception and signaling events of ethylene.

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Section: Sprout Suppression Through Exogenous Ethylene Down-regulatessupporting

confidence: 90%

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confidence: 95%

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Ethylene and 1-Methylcyclopropene Differentially Regulate Gene Expression during Onion Sprout Suppression

et al. 2011

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“…For comparison, 20 % of bulbs of the next most storable cultivars 'Magic Gold' and 'New Turbo' sprouted for the same period of time (Nam et al 2011). Onset of sprouting is commonly preferred to characterize the storage life of onions because of its clear relationship to physiological processes in bulbs (Pak et al 1995;Chope et al 2007a;Yasin and Bufler 2007;Bufler 2009). In the present work, the development of inner sprouts was observed on longitudinally cut bulbs after 2 months of storage and visible sprouts appeared after 5 months in a good agreement with the above mentioned results of Nam et al (2011).…”

Section: Resultsmentioning

confidence: 99%

Quantitative analysis of flavonoids, sugars, phenylalanine and tryptophan in onion scales during storage under ambient conditions

Sharma

Lee

et al. 2013

J Food Sci Technol

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A comprehensive quantitative analysis of flavonoids, sugars, phenylalanine, and tryptophan have been carried out in different onion scales during storage at ambient temperature (20-23°C) and relative humidity (60-80 %). Depending on the length of storage, dry matter content and composition shows variation inside the onion bulbs. Inner sprouts were observed on longitudinally cut bulbs after 2 months and visible sprouts appeared after 5 months of storage. The bulbs lost 20 to 30 % of their weight at the end of the storage. Higher dry matter content was observed in the inner scales. Significantly high content of quercetin in inner scales and high level of quercetin-3,4′-O -diglucoside and quercetin-4′-O-monoglucoside in outer scales was observed during a 7 months storage. During storage period, high content of fructose and glucose was observed in the middle scales while sucrose was high in the inner scales. There was no particular trend observed within analyzed amino acids. However, the content of phenylalanine was higher than tryptophan.

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“…En condiciones naturales, la brotación de los bulbos de cebolla chalote ocurre luego de un cierto tiempo después de la cosecha dependiendo del genotipo y las condiciones de almacenamiento, debido a que éstos tienen un periodo de dormancia [26]. Estudios de Benkeblia y colaboradores [27] demostraron que los compuestos fenólicos interfieren indirectamente con el proceso de brotación de bulbos de cebolla y que una baja temperatura (0 • C) activa una señal para reducir fenoles, con lo cual se promueve la brotación de bulbos.…”

Section: Discussionunclassified

Estandarización de un protocolo de regeneración de cebolla chalote (<em>Allium cepa</em> var. <em>aggregatum</em>) a partir de meristemas apicales

Vega

Arahana

Torres

2015

Av. Cienc. Ing. (Quito)

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Shallots (Allium cepa var. aggregatum) are a type of onion which form several bulbs from a single basal disc. They are characterized by a high total solids content which improves their gastronomic and neutraceutical properties. Shallots (Allium cepa var. aggregatum) are a culinary staple with high commercial value in European, North American and South American markets. Their commercial production via vegetative propagation, however, is time-consuming and strongly affected by viral diseases. The aim of this study was to establish an in vitro apical meristem culture protocol for the regeneration of shallot plants. The efficiency of different regeneration and bulb-formation media were evaluated in this investigation. The best results for plant regeneration (68 % efficiency) were obtained with MS + 30g.L −1 sucrose + 1.5g.L −1 benomyl + 1.1uM NAA + 8.9uM BAP and for bulb formation (77 % efficiency) with MS + 10uM ancymidol and 50g.L −1 sucrose. Moreover, 50 % of all plants regenerated from apical meristems were effectively acclimatized. The protocol developed and standardized in this investigation can be used to increase the efficiency of shallot bulb-seed production for commercial applications.Keywords. Allium cepa var. aggregatum, apical bud, in vitro culture. ResumenLa cebolla chalote (Allium cepa var. aggregatum) forma varios bulbos a partir de un solo disco basal y tiene mayor contenido de sólidos totales que aumentan su valor gastronómico y nutracéutico. Es un producto altamente valorado en el mercado europeo, estadounidense y sudamericano. En el Ecuador no se la registra como una variedad diferente a la cebolla roja. Sin embargo, la cebolla chalote es apetecida y utilizada dentro del sector gastronómi-co y aumenta el interés de su producción. El objetivo de este estudio fue estandarizar un protocolo de regeneración in vitro de plantas de chalote a partir de meristemas apicales. Se aisló meristemas apicales a partir de bulbos de chalote, se probó varios medios para la regeneración de plantas y la formación de bulbos. El porcentaje de regeneración de plantas más alto (68 %) se obtuvo con el medio: MS + 30g.L −1 sacarosa, 1.5g.L −1 benomyl + 1.1uM NAA + 8.9uM BAP, y de embulbamiento (77 %) con el medio: MS + 10uM ancymidol + 50g.L −1 sacarosa. Finalmente, el 50 % de plantas regeneradas a partir de meristemas fueron aclimatadas eficientemente. El protocolo establecido en esta investigación puede ser utilizado para mejorar la producción de bulbo-semilla y así promover el cultivo de cebolla chalote en el país.Palabras Clave. Allium cepa var. aggregatum, meristema apical, cultivo in vitro. IntroducciónAllium cepa var. aggregatum se caracteriza porque forma grupos de bulbos unidos por un mismo disco basal, mientras que la cebolla común (Allium cepa L.) solo forma un bulbo principal. Es originaria del centro y sudeste de Asia, y actualmente está distribuida en todo el mundo [1]. Posee un mayor contenido de sólidos (16-33 %) que la cebolla común (7-15 %), entre los que se menciona ácidos grasos, azúcares y comp...

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Exogenous ethylene inhibits sprout growth in onion bulbs

Abstract: Exogenous ethylene proved to be a powerful inhibitor of sprout growth in onion bulbs. The dormancy breaking effect of 1-MCP indicates a regulatory role of endogenous ethylene in onion bulb dormancy.

Cited by 34 publications

References 16 publications

Ethylene and 1-Methylcyclopropene Differentially Regulate Gene Expression during Onion Sprout Suppression

Ethylene and 1-Methylcyclopropene Differentially Regulate Gene Expression during Onion Sprout Suppression

Quantitative analysis of flavonoids, sugars, phenylalanine and tryptophan in onion scales during storage under ambient conditions

Estandarización de un protocolo de regeneración de cebolla chalote (<em>Allium cepa</em> var. <em>aggregatum</em>) a partir de meristemas apicales

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