Exogenous dibutyryl cAMP affects meiotic maturation via protein kinase A activation; it stimulates further embryonic development including blastocyst quality in pigs

Kim, Ji Su; Cho, Yeesook; Song, Bong-Seok; Wee, Gabbine; Park, Jung Sun; Choo, Young‐Kug; Yu, Kweon; Lee, Kyung Kwang; Han, Yong‐Mahn; Koo, Deog‐Bon

doi:10.1016/j.theriogenology.2007.09.024

Cited by 34 publications

(32 citation statements)

References 31 publications

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“…From these results, we can deduce that dbcAMP addition is beneficial to obtain more normally fertilized zygotes within the penetrated oocytes. The detected decrease in polyspermy rate after the use of dbcAMP confirms the findings from Somfai et al [13] and Kim et al [15] but contrasts the study of Funahashi et al [14] who found a similar polyspermy rate. The lower penetration rate in our study conflicts the results of the three studies mentioned before in which the same [13,14] or a higher penetration rate [15] was found.…”

Section: Discussionsupporting

confidence: 85%

“…Funahashi et al [14] pointed out that dbcAMP did not influence penetration and polyspermy rate, Somfai et al [13] found similar penetration rates but lower polyspermy rates, and Kim et al [15] measured lower polyspermy rates but higher penetration rates. Decreasing polyspermy rates after IVF of cumulusintact oocytes can be due to an improved cumulus expansion [16].…”

Section: Introductionmentioning

confidence: 99%

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Increasing the cAMP concentration during in vitro maturation of pig oocytes improves cumulus maturation and subsequent fertilization in vitro

et al. 2015

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Increasing the cAMP concentration during in vitro maturation of pig oocytes improves cumulus maturation and subsequent fertilization in vitro

et al. 2015

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“…In IVM medium containing gonadotropins, the effects of dibutyryl cAMP have been well documented. Treatment of prepubertal COCs with dibutyryl cAMP during the first half of IVM improves the synchronicity of meiotic resumption and progression [19] and the developmental competence of porcine oocytes following IVF [19,20,35] by increasing the mitochondrial membrane potential and decreasing the incidence of apoptosis in preimplantation-stage porcine embryos [36]. This evidence shows that control of meiotic resumption in porcine oocytes is highly regulated by cAMP [37].…”

Section: Discussionmentioning

confidence: 69%

Successful Piglet Production in a Chemically Defined System for In-vitro Production of Porcine Embryos: Dibutyryl Cyclic AMP and Epidermal Growth Factor-family Peptides Support In-vitro Maturation of Oocytes in the Absence of Gonadotropins

Akaki

Yoshioka

Noguchi

et al. 2009

Journal of Reproduction and Development

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Abstract.To induce meiotic resumption of porcine oocytes, it is thought to be necessary to expose the cumulus-oocyte complexes (COCs) to gonadotropins during in-vitro maturation (IVM). However, the detailed mechanism of meiotic resumption by gonadotropins is still unknown, and successful piglet production has not been reported by using oocytes matured in gonadotropin-free media and fertilized in vitro. The present study was undertaken to examine the combinational effects of epidermal growth factor (EGF)-family members and dibutyryl cyclic AMP (cAMP) in a chemically defined medium on IVM of porcine oocytes and the developmental competence following in vitro fertilization (IVF). The basic IVM medium was a chemically defined medium, modified porcine oocyte medium (mPOM). Supplementation of the IVM medium with 10 or 1000 ng/ml EGF, amphiregulin and betacellulin during the whole IVM period, except for 10 ng/ml amphiregulin, increased the percentage of oocytes maturing to the metaphase-II stage. When COCs were exposed to both dibutyryl cAMP and EGF-family members during the first 20-h of IVM and then culture was continued in the absence of EGF-family members and dibutyryl cAMP, the incidence of metaphase-II oocytes was significantly increased and was not different from that of oocytes cultured in a standard IVM system with gonadotropins. The developmental competence of the oocytes to the blastocyst stage following IVF was no different from that of control oocytes matured with gonadotropins. When these blastocysts were transferred into the uterine horn of three recipients, all of gilts became pregnant and delivered a total of 11 piglets. These observations indicate that supplementation of a chemically defined maturation medium with EGF-family members and dibutyryl cAMP during the first 20 h of IVM can support well the meiotic progress and developmental competence of porcine oocytes. Key words: Cyclic AMP (cAMP), Epidermal growth factor (EGF), In vitro maturation, Oocyte, Pig (J. Reprod. Dev. 55: [446][447][448][449][450][451][452][453] 2009) uccessful in-vitro production of porcine embryos has the potential to provide an effective system for mass production of embryos for better understanding of the physiology of embryonic development and animal reproductive technologies, including production of transgenic and/or cloned animals [1,2]. Relatively efficient systems for in-vitro production of porcine embryos have already been developed, but they contain materials derived from organisms, such as porcine follicular fluid and bovine serum albumin, in the culture media [3,4]. Chemically defined media, which only known chemicals, but none derived from animals, yeast or plants, should be useful in clarifying the mechanism of interactive communication between the oocyte and the surrounding cumulus cells during meiosis, the oocyte reaction following sperm penetration and early development. Furthermore, application of a defined system for in-vitro embryo production would be good for a quality control against the risk of viral c...

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“…In addition, it has been suggested that when treated with dbcAMP for the first 22 h of maturation, most pig oocytes arrest in the GV stage. However, after removal of dbcAMP, these oocytes exhibit enhanced meiotic resumption and embryonic quality after fertilization [24,25]. This indicates that synchronizing meiotic resumption by dbcAMP treatment improves the developmental capacity of the embryo.…”

Section: Discussionmentioning

confidence: 99%

Effect of Vanadate on the Chromatin Configuration in Pig GV-oocytes

Bui

Hwang

Kim

et al. 2009

Journal of Reproduction and Development

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Abstract.Vanadate, an inhibitor of tyrosine phosphatases, has been reported to prevent germinal vesicle breakdown in mammalian oocytes. We examined the effect of vanadate on the chromatin configuration of fully grown pig oocytes. In the presence of human menopausal gonadotropin (hMG), vanadate (0.5-5 mM) resulted in a dose-dependent change in oocyte chromatin in germinal vesicles from the condensed state to a decondensed filamentous or stringy configuration. The effect of vanadate and hMG on chromatin configuration could be replicated with 2 mM dibutyryl cyclic AMP (dbcAMP) in place of hMG. Western blot analysis showed that vanadate caused a massive accumulation in the oocytes of tyrosine-phosphorylated proteins with a range of molecular weights that was enhanced by both hMG and dbcAMP in a similar manner. These results suggest that inhibition of tyrosine phosphatase(s) in the presence of an effective level of cAMP induces a change in chromatin configuration of pig oocytes. Key words: Cyclic AMP (cAMP), Oocyte Maturation, Pig, Vanadate (J. Reprod. Dev. 55: [367][368][369][370][371][372] 2009) ully grown mammalian oocytes arrested at prophase I resume meiosis spontaneously in vitro when they are released from the inhibitory influences of their surrounding follicles [1]. Because meiotic resumption is inhibited by membrane-permeable cAMP analogs, such as dibutyryl cAMP (dbcAMP), and inhibitors of cyclic nucleotide phosphodiesterase, such as 3-isobutyl-1-methylxanthine (IBMX), it is thought that a sustained high level of cAMP inhibits this process in oocytes within the follicles [2]. In this environment, meiotic resumption is triggered by the preovulatory gonadotropic surge from the pituitary [3]. Gonadotropins promote meiotic resumption of oocytes via an indirect mechanism mediated by surrounding cumulus granulosa cells rather than by a direct action on the oocytes [4].A burst of phosphorylation of a range of proteins occurs in oocytes during meiotic resumption [5,6]. The key kinase that phosphorylates many cellular substrates is a maturation-promoting factor (MPF), a serine/threonine protein kinase consisting of a catalytic subunit, Cdc2, and a regulatory subunit, cyclin B [7,8]. Protein tyrosine phosphatases (PTPases) have also been suggested to be involved in meiotic resumption of oocytes. Vanadate, a potent inhibitor of tyrosine phosphatases, has been used to study the role of PTPases in oocytes and somatic cells [9]. In mouse and rat oocytes, vanadate prevents germinal vesicle breakdown by inhibiting tyrosine dephosphorylation of Cdc2 [10,11]. In pig oocytes, Aquino et al. have reported that vanadate inhibits germinal vesicle breakdown almost completely at concentrations of 250 μM and 500 μM [12].We repeated this experiment using pig oocytes and found that vanadte induced changes in chromatin configuration in arrested germinal vesicles. In the present study, we cultured fully grown pig oocytes with vanadate at different concentrations and examined the precise change in chromatin configuration. The results s...

show abstract

Exogenous dibutyryl cAMP affects meiotic maturation via protein kinase A activation; it stimulates further embryonic development including blastocyst quality in pigs

Cited by 34 publications

References 31 publications

Increasing the cAMP concentration during in vitro maturation of pig oocytes improves cumulus maturation and subsequent fertilization in vitro

Increasing the cAMP concentration during in vitro maturation of pig oocytes improves cumulus maturation and subsequent fertilization in vitro

Successful Piglet Production in a Chemically Defined System for In-vitro Production of Porcine Embryos: Dibutyryl Cyclic AMP and Epidermal Growth Factor-family Peptides Support In-vitro Maturation of Oocytes in the Absence of Gonadotropins

Effect of Vanadate on the Chromatin Configuration in Pig GV-oocytes

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