Exogenous Autoinducer-2 Rescues Intestinal Dysbiosis and Intestinal Inflammation in a Neonatal Mouse Necrotizing Enterocolitis Model

Ji, Yan-Chun; Sun, Qian; Fu, Chenbo; She, Xiang; Liu, Xiao-Chen; He, Yu; Ai, Qing; Li, Lu‐Quan; Wang, Zhengli

doi:10.3389/fcimb.2021.694395

Cited by 22 publications

(26 citation statements)

References 37 publications

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“…Similarly, the abundance of Clostridium_sensu_stricto_1 in PG was also high (Fig. 2b), which was similar to those reports that its abundance increases signi cantly when the intestinal microorganisms are out of imbalance [42,43]. It is speculated that lung in ammation may affect the composition of intestinal microorganisms.…”

Section: Discussionsupporting

confidence: 87%

Comparison of the gut microflora of common kestrel (Falco tinnunculus) according to differences in sex, age, and physiological condition

Zhang

Gong

Wang

et al. 2022

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Background Gut microbes play an important role in animal health, and their compositions and changes can reflect the physical condition of animals. However, the gut microbes of most birds, especially raptors, have been poorly investigated. This study used 16S rRNA gene sequencing technology to determine the gut microbiome of rescued common kestrels, compared the composition and diversity of the gut microbiome of common kestrels under different physiological conditions, and analyzed the effects of sex and age on the gut microbes of common kestrels. Results The results showed that Proteobacteria and Firmicutes were the dominant bacteria at the phylum level among the gut flora of kestrels. Difference in the physiological condition of kestrels exerted a significant influence on their gut microbe composition. Specifically, compared with the healthy group, the abundance of Lactobacillus in the fracture group increased, and the interaction between functionally similar flora was strengthened; in the pneumonia group, the abundance of potentially pathogenic microorganisms such as Clostridium_sensu_stricto_1 and Paeniclostridium was significantly enhanced (P < 0.05). Moreover, the interaction of differences in physiological condition and sex had a significant impact on the abundance of Proteobacteria and Actinobacteria in the guts of kestrels. In addition, the abundance of Acinetobacter was significantly affected by age, and with increasing age, its abundance also increased. Conclusions This study showed that sex, age and physiological condition affected the composition and diversity of the gut microbiome of common kestrels, and specific microorganisms changed in abundance in individuals with bacterial pneumonia or fractures, which provides a new perspective for the disease diagnosis and treatment of wild common kestrels.

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Section: Discussionsupporting

confidence: 87%

Comparison of the gut microflora of common kestrel (Falco tinnunculus) according to differences in sex, age, and physiological condition

Zhang

Gong

Wang

et al. 2022

Preprint

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“…The relative abundance of Clostridium_sensu _stricto_1 was significantly increased in the gut microecological imbalance. Ji et al [ 31 ] found significant enrichment of Clostridium_sensu_stricto_1 in a model of necrotic enterocolitis in neonatal mice. It was also found to be significantly enriched in IBS syndrome [ 32 ] and leukemia [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

The difference of gut microbiome in different biliary diseases in infant before operation and the changes after operation

et al. 2022

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Background Evidence supports an association between cholestatic liver disease and changes in microbiome composition. Nevertheless, the identification of this special type of biliary atresia from non-biliary atresia cholestasis is still a major clinical difficulty. The purpose of this study is to compare the differences in the composition of gut microbiome between infants with biliary atresia and infant with non-biliary atrestic cholestasis, to find new ways to identify and diagnose these two diseases early, to understand the influence of the presence or absence of bile on the composition of the gut microbiome in infants with cholestasis. Methods Using 16S rDNA gene sequencing technology to analyze the intestinal flora of the participants. Results In terms of diversity, there is an obvious structural separation in the intestinal microbiota of the BA group and the CD group, and this structural separation also exists in the comparison between the two groups before surgery. Taxonomic analysis demonstrated that the two groups showed an increase in Proteobacteria and Firmicutes before surgery, and the relative abundance of potential pathogens such as Shigella, Streptococcus, Klebsiella, etc. increased, potential probiotics such as Bifidobacteria and Lactobacillus decreased, but the relative abundance of each genus was different between groups. It was found that Enterococcus, Ralstonia, Nitriliruptoraceae, etc. were differentially enriched in the BA group, the CD group are mainly enriched in Veillonella, Clostridium_sensu_stricto_1 and Lactobacillus. Functional analysis of the groups showed that the BA group mainly focused on the processes of energy release processes, and the CD group mainly focused on the biosynthesis of amino-acids to consume energy. Conclusions The composition of intestinal flora is different between biliary atresia and non-biliary atretic cholestasis. Enterococcus, Ralstonia, etc. may become biomarkers for the identification and diagnosis of both.

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“…The proin ammatory cytokines TNF-α, IL-1β, and IL-6 were elevated in infants and animal models with NEC (45,46), which can be suppressed by the use of TLR4 inhibitors (8, 47). Our experiment showed that early supplement with β-glucan reduced TLR4 and NF-κB protein expression levels in NEC mice.…”

Section: Discussionmentioning

confidence: 99%

β-glucan protects against necrotizing enterocolitis in mice by inhibiting intestinal inflammation, improving gut barrier and modulating gut microbiota

Zhang

et al. 2022

Preprint

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Background Necrotizing enterocolitis (NEC) is a devastating gastrointestinal disease with high morbidity and mortality, affecting preterm infants especially those with very low and extremely low birth weight. β-glucan has manifested of multiple biological effects including anti-inflammatory, regulating gut microbiota and immunomodulatory activities. At present, there is no relevant study considering the effects of β-glucan on NEC. This study aimed to investigate the effects of β-glucan on NEC. Methods Neonatal C57BL/6 mice were randomly divided into three groups: control group, NEC group and β-glucan group. Newborn 3-day-old mice were gavage with either 1mg/ml β-glucan or PBS at 0.03 ml/g for consecutive 7 days before NEC induction and a NEC model was established with hypoxia combined with cold exposure and formula feeding. All the pups were killed after 72-hour modeling. HE staining was performed to assess the pathological injury of the intestine. The mRNA expression levels of inflammatory factors in intestinal tissues were determined by quantitative real-time PCR. The protein levels of TLR4, NF-κB and tight junctions proteins in intestinal tissues were evaluated using western blotting and immunohistochemistry. 16S rRNA sequencing was performed to determine the structure of gut microbiota. Results β-glucan administration ameliorated intestinal injury of NEC mice; reduced the intestinal expression of TLR4, NF-κB, interleukin- (IL-) 1β, IL-6 and TNF-α; and increased the intestinal expression of IL-10 (P < 0.05); improved the expression of ZO-1, Occludin and Claudin-1 within intestinal barrier. Pre-treatment with β-glucan also increased the proportion of Actinobacteria, Clostridium_butyricum, Lactobacillus_johnsonii,Lactobacillus_murinus and Lachnospiraceae_bacterium_mt14 and reduced the proportion of Klebsiella_oxytoca_g__Klebsiella in the NEC model. Conclusion β-glucan intervention can prevent necrotizing enterocolitis in neonatal mice, possibly by suppressing TLR4-NF-κB signaling pathway, improving intestinal barrier function and partially regulating intestinal microbiota.

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Exogenous Autoinducer-2 Rescues Intestinal Dysbiosis and Intestinal Inflammation in a Neonatal Mouse Necrotizing Enterocolitis Model

Cited by 22 publications

References 37 publications

Comparison of the gut microflora of common kestrel (Falco tinnunculus) according to differences in sex, age, and physiological condition

Comparison of the gut microflora of common kestrel (Falco tinnunculus) according to differences in sex, age, and physiological condition

The difference of gut microbiome in different biliary diseases in infant before operation and the changes after operation

β-glucan protects against necrotizing enterocolitis in mice by inhibiting intestinal inflammation, improving gut barrier and modulating gut microbiota

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