Exogenous application of rosmarinic acid improves saccharification without affecting growth and lignification of maize

Bevilaqua, Jennifer Munik; Finger-Teixeira, Aline; Marchiosi, Rogério; Oliveira, Dyoni Matias de; Joia, Breno Miguel; Ferro, Ana Paula; Parizotto, Ângela Valderrama; Santos, Wanderley Dantas dos; Ferrarese-Filho, Osvaldo

doi:10.1016/j.plaphy.2019.07.015

Cited by 16 publications

(8 citation statements)

References 43 publications

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“…p‐ Hydroxycinnamate‐CoA ligase (4CL) activity was carried out as described by Bevilaqua et al (2019), using p CA or FA as substrates. The extraction buffer contained 200 mM Tris–HCl (pH 7.5), 10 mM MgCl 2, 5 mM DTT and 20% glycerol ( vol/vol ).…”

Section: Methodsmentioning

confidence: 99%

“…Control experiments without substrates were performed under the same conditions to identify any endogenous compounds in the enzymatic extracts. p-Hydroxycinnamate-CoA ligase (4CL) activity was carried out as described by Bevilaqua et al (2019), using pCA or FA as substrates.…”

Section: Enzymatic Assaysmentioning

confidence: 99%

“…Caffeic acid 3-O-methyltransferase (COMT) activity was performed as described by Bevilaqua et al (2019). The extraction buffer contained 100 mM Tris-HCl (pH 7.2), 0.2 mM MgCl 2, 2 mM DTT and 10% glycerol (vol/vol).…”

Section: Enzymatic Assaysmentioning

confidence: 99%

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Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize

Oliveira

Mota

Salatta

et al. 2020

Plant Cell & Environment

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Although cell wall polymers play important roles in the tolerance of plants to abiotic stress, the effects of salinity on cell wall composition and metabolism in grasses remain largely unexplored. Here, we conducted an in-depth study of changes in cell wall composition and phenolic metabolism induced upon salinity in maize seedlings and plants. Cell wall characterization revealed that salt stress modulated the deposition of cellulose, matrix polysaccharides and lignin in seedling roots, plant roots and stems. The extraction and analysis of arabinoxylans by size-exclusion chromatography, 2D-NMR spectroscopy and carbohydrate gel electrophoresis showed a reduction of arabinoxylan content in salt-stressed roots. Saponification and mild acid hydrolysis revealed that salinity also reduced the feruloylation of arabinoxylans in roots of seedlings and plants. Determination of lignin content and composition by nitrobenzene oxidation and 2D-NMR confirmed the increased incorporation of syringyl units in lignin of maize roots. Salt stress also induced the expression of genes and the activity of enzymes enrolled in phenylpropanoid biosynthesis. The UHPLC-MS-based metabolite profiling confirmed the modulation of phenolic profiling by salinity and the accumulation of ferulate and its derivatives 3-and 4-O-feruloyl

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Section: Methodsmentioning

confidence: 99%

Section: Enzymatic Assaysmentioning

confidence: 99%

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Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize

Oliveira

Mota

Salatta

et al. 2020

Plant Cell & Environment

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“…32 Under reflux conditions, the intermediate B was treated with 3chlorodihydrazide pyridine in n-butanol to obtain the intermediate C. 33 Intermediate C was reacted with triethyl orthoformate to obtain intermediate D. 34 The intermediate E with high yield can be easily obtained at room temperature when hydrazine hydrate (80%) and intermediate D were treated in ethanol. 20,48 Using intermediate E as the raw material, in the presence of glacial acetic acid, it reacted with different aldehydes under the reflux of ethanol to obtain F1−F17. The intermediate F was reduced by NaBH 4 to obtain target compounds G1−G17.…”

Section: Resultsmentioning

confidence: 99%

Synthesis of 4H-Pyrazolo[3,4-d]pyrimidin-4-one Hydrazine Derivatives as a Potential Inhibitor for the Self-Assembly of TMV Particles

Wang,

Guo,

Sun

et al. 2024

J. Agric. Food Chem.

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Tobacco mosaic virus coat protein (TMV-CP), as a potential target for the development of antiviral agents, can assist in the long-distance movement of viruses and plays an extremely important role in virus replication and propagation. This work focuses on the synthesis and the action mechanism of novel 4H-pyrazolo[3,4-d] pyrimidin-4-one hydrazine derivatives. The synthesized compounds exhibited promising antiviral activity on TMV. Specifically, compound G2 exhibited high inactivating activity (93%) toward TMV, slightly better than commercial reagent NNM (90%). The action of mechanism was further explored by employed molecular docking, molecular dynamics simulation, microscale thermophoresis, qRT-PCR, and transmission electron microscopy. Results indicated that G2 had the capability to interact with amino acid residues such as Trp352, Tyr139, and Asn73 in the active pocket of TMV-CP, creating strong hydrophobic interactions and thus obstructing the virus's self-assembly.

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“…4 Additionally, they are naturally occurring bioactive chemicals that have key functions in various metabolic activities, including plant growth, cell differentiation, photosynthetic productivity, saccharization, and the modulation of defense responses. 5 Besides, these compounds serve as natural defense mechanisms against pathogens, acting as phytoalexins to inhibit the growth of harmful microorganisms, and contribute to allelopathy by enabling plants to release chemicals that suppress neighboring plant growth. The phenolic compounds elicit the production and activation of bioactive compounds through intricate molecular mechanisms via regulating gene expression and influencing the synthesis of various secondary metabolites.…”

Section: Introductionmentioning

confidence: 99%

The exogenous application of naringenin and rosmarinic acid modulates functional traits in Lepidium sativum

Salehi,

Zhang,

Alp‐Turgut

et al. 2023

J Sci Food Agric

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BACKGROUNDPhenolic modulators have attracted attention for their potential in shaping functional traits in plants. This work investigated the impact of naringenin (Nar) and rosmarinic acid (RA) on the functional properties of Lepidium sativum leaves and roots.ResultsUntargeted metabolomics identified a diverse phenolic profile, including flavonoids, phenolic acids, low molecular weight phenolics, lignans, and stilbenes. Cluster, analysis of variance multiblock orthogonal partial least squares (AMOPLS), and orthogonal projection to latent structures discriminant analysis (OPLS‐DA) multivariate analyses confirmed tissue‐specific modulation of bioactive compounds. The tissue was the hierarchically most influential factor, explaining 27% of observed variability, while the treatment and their interaction were statistically insignificant. Thereafter, various in vitro assays were employed to assess antioxidant capacity, including 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH), 2,2′‐azino‐bis(3‐ethylbenzothiazoline)‐6‐sulfonic acid (ABTS) radical scavenging activity, cupric ion reducing antioxidant capacity (CUPRAC), and ferric ion reducing antioxidant power (FRAP), metal chelating ability, and phosphomolybdenum (PMD) assays. Extracts were also tested for inhibitory effects on cholinesterase, amylase, glucosidase, and tyrosinase enzymes. RA application positively impacted antioxidant and enzyme inhibitory activities, holding valuable implications in shaping the health‐promoting properties of L. sativum.ConclusionThe untargeted metabolomics analysis showed a significant tissue‐dependent modulation of bioactive compounds, determining no synergistic effect between applying phenolic compounds in combination. Specifically, the sole application of RA increased anthocyanins and hydroxyphenyl propanoic acid content on leaves, which was strictly related to enhancing the biological activities. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Exogenous application of rosmarinic acid improves saccharification without affecting growth and lignification of maize

Cited by 16 publications

References 43 publications

Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize

Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize

Synthesis of 4H-Pyrazolo[3,4-d]pyrimidin-4-one Hydrazine Derivatives as a Potential Inhibitor for the Self-Assembly of TMV Particles

The exogenous application of naringenin and rosmarinic acid modulates functional traits in Lepidium sativum

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