Exogenous application of ethylenediamminetetraacetic acid enhanced phytoremediation of cadmium by Brassica napus L.

Abstract

Performance of B. napus in phytoextractionan in situ environment friendly technique for the cleanup of contaminated soils-was evaluated through its response to cadmium (Cd) toxicity in combination with a chelator ethylenediamminetetraacetic acid (EDTA) while growing hydroponically in greenhouse conditions under three levels of Cd (0, 10, and 50 lM) and two levels of EDTA (0 and 2.5 mM). Cadmium presence decreased plant growth, biomass and chlorophyll concentrations, while the application of EDTA enhanced plant growth by reducing Cdinduced effects in Cd-stressed plants. Addition of EDTA improved the net photosynthetic and gas exchange capacity of plants under Cd stress. Presence of Cd at 10 and 50 lM significantly increased electrolyte leakage, the production of hydrogen peroxidase (H 2 O 2 ) and malondialdehyde (MDA) resulting into a significant reduction in the activities of catalase, guaiacol peroxidase, ascorbate peroxidase and superoxide dismutase in Cd-stressed plants. Application of EDTA at the rate of 2.5 mM alone and with combination of Cd increased the antioxidant enzymes activities and reduced the electrolyte leakage and production of H 2 O 2 and MDA. The B. napus actively accumulated Cd when applied with EDTA in roots, stems and leaves viz. 2817, 2207 and 1238 mg kg -1 DW, respectively, at higher Cd level (50 lM) followed by lower level of Cd (10 lM) viz. 1704, 1366 and 763 mg kg -1 DW, respectively. Results showed that this technique could be useful for the remediation of heavy metal-contaminated agricultural and industrial soils.

EDTA reduces cadmium toxicity in mustard (Brassica juncea L.) by enhancing metal chelation, antioxidant defense and glyoxalase systems

“…content of the Cd-treated plants. Our results are in agreement with those of Niakan and Kaghazloo [45] and Farid et al [16], who reported that EDTA increases the content of chl in Helianthus annuus L. and B. napus L. plants. Cadmium stress is also associated with other changes in metabolism including water and nutrient uptake, inhibition of enzyme activity, reduction of photosystem efficiency and photosynthetic rate, which all result in growth inhibition [36,42,44].…”

“…In our study, the growth of the Cd-affected seedlings (shoot length, fresh and dry weight) decreased significantly, in comparison to control seedlings, which is similar to earlier reports [41,44]. Ethylenediaminetetraacetic acid significantly restores the growth and biomass of B. napus by reducing Cd-induced inhibition and increasing plant resistance towards Cd stress [16]. Similarly, Agbadah et al [18] exposed Zea mays L. and V. unguiculata plants to Cd in combination with EDTA and found that EDTA considerably increased the stem length and fresh weight of Cd-stressed plants.…”

“…Supplementation of EDTA to the Cd-stressed plants also enhanced the enzyme activity of the AsA-GSH cycle (APX, MDHAR, DHAR, and GR). These results are almost the same as those of previous studies [16,19,22]. Farid et al [16] recorded that treating Cd-stressed plants with EDTA notably increased protein content and antioxidant enzyme activity, whilst it reduced TBARS and H 2 O 2 contents by scavenging ROS.…”

Measurement of Different Heavy Metals Concentration in Roadside Dust in the Vicinity of Gujrat, Pakistan

“…These heavy metals has ability to circulate in all media of environment by biomagnifications and bioaccumulation. These not just pollute the soil but also accumulate in food plants [32,19,24] and intake by human and animals during food consumption and cause health related problems [35,1]. Traffic pollution has emerged as a serious issue due to potential hazard on human health and nearby environment.…”

Leaching risk of chelate induced phytoremediation of Tl contaminated soil by Zea mays L.

“…in the soil, and can promote the effective absorption of plants. (2) Typical tolerant plants are mainly :Maize,Vetiver grass,Cabbage and Chrysanthemum [2][3][4] . Among them, the maize(such as ZhengDan 958),a kind of a well-tolerated plants , is determined to be able to "side of the production side of the repair" and is worth promoting [5] .…”