Exoelectrogenic Bacterium Phylogenetically Related to Citrobacter freundii, Isolated from Anodic Biofilm of a Microbial Fuel Cell

Huang, Jinsha; Zhu, Nengwu; Cao, Yanlan; Yue, Peng; Wu, Pingxiao; Dong, Wenhao

doi:10.1007/s12010-014-1418-9

Cited by 42 publications

(10 citation statements)

References 35 publications

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“…have also been observed in MFC biocathodes and a highly productive methanogenic biocathode supplemented with zero valent iron. ,, Other putative exoelectrogens were also detected at a higher relative abundance in the biogas-fed biocathode than in the CO 2 -fed biocathode. Ochrobactrum and Citrobacter species have previously been isolated from MFC bioanodes and identified as exoelectrogens. ,− In the present study, Ochrobactrum spp. and Citrobacter spp.…”

Section: Resultsmentioning

confidence: 50%

Comparison of Carbon Dioxide with Anaerobic Digester Biogas as a Methanogenic Biocathode Feedstock

Dykstra

Cheng

Pavlostathis

2020

Environ. Sci. Technol.

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BES biogas upgrading studies have typically used bicarbonate or commercial gas mixtures as a biocathode substrate instead of anaerobic digester biogas. Therefore, the objective of this study was to (i) compare the performance of a methanogenic BES between CO 2 -fed and biogas-fed cycles; (ii) investigate possible factors that may account for observed performance differences; and (iii) assess the performance of a biogas-fed biocathode at various applied cathode potentials. The maximum 1-d CH 4 production rate in a biogas-fed biocathode (3003 mmol/m 2 -d) was 350% higher than in a CO 2 -fed biocathode (666 mmol/m 2 -d), and the biogas-fed biocathode was capable of maintaining high performance despite a variable biogas feed composition. Anode oxidation of reduced gases (e.g., CH 4 and H 2 S) from biogas may theoretically contribute 4% to 35% of the total charge transfer from anode to cathode at applied cathode potentials of −0.80 to −0.55 V (vs SHE). The introduction of biogas did not significantly change the biocathode archaeal community (dominated by a Methanobrevibacter sp. phylotype), but the bacterial community shifted away from Bacteroidetes and toward Proteobacteria, which may have contributed to the improved performance of the biogas-fed system. This study shows that anaerobic digester biogas is a promising biocathode feedstock for BES biogas upgrading.

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Section: Resultsmentioning

confidence: 50%

Comparison of Carbon Dioxide with Anaerobic Digester Biogas as a Methanogenic Biocathode Feedstock

Dykstra

Cheng

Pavlostathis

2020

Environ. Sci. Technol.

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“…The present study exhibited a maximum power density of 212 mW/m 2 ( Figure 5 ) with a recovery of 13.3% ( Figure 6 ) as an electrical current using the strain KVM11 in acetotrophic conditions. Such findings suggest that the strain KVM11 is also capable of utilizing insoluble electron acceptors with the additional distinctive features making this strain unique from its counterparts ( Xu and Liu, 2011 ; Huang et al, 2015 ; Liu et al, 2016 ), which include, (i) ability to degrade PH, (ii) dye detoxification in MERS, and (iii) anoxic Fe(III) reduction. The typical polarization curve of Citrobacter strain KVM11 ( Figure 7 ) in an acetate-fed MFC indicated a large potential drop due to activational losses as shown in other exoelectrogenic strains ( Park et al, 2001 ; Pham et al, 2003 ; Venkidusamy and Megharaj, 2016a , b ).…”

Section: Resultsmentioning

confidence: 98%

“…With regards to the Citrobacter strains, for example, Citrobacter sp. LAR-1 ( Liu et al, 2016 ) and C. freundii Z7 ( Huang et al, 2015 ) have also shown to be Fe(III) reducing exoelectrogens, although the rate of Fe(III) reduction is unknown. The strain KVM11 displayed a wide nutritional spectrum as highlighted by its utilization of various carbon sources under anaerobic conditions from its counterparts, Citrobacter sp.…”

Section: Resultsmentioning

confidence: 99%

“…The strain KVM11 displayed a wide nutritional spectrum as highlighted by its utilization of various carbon sources under anaerobic conditions from its counterparts, Citrobacter sp. LAR-1 ( Liu et al, 2016 ) C. freundii Z7 ( Huang et al, 2015 ) ( Table 1 ). However, the strain showed a different carbon source profile than the previously reported strains of Citrobacter with regards to its ability to assimilate a range of substrates including alanine, phenylalanine, adonitol, aminobutyric acid, lactose, etc., ( Brenner et al, 1993 , 1999 ).…”

Section: Resultsmentioning

confidence: 99%

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Petrophilic, Fe(III) Reducing Exoelectrogen Citrobacter sp. KVM11, Isolated From Hydrocarbon Fed Microbial Electrochemical Remediation Systems

2018

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Exoelectrogenic biofilms capable of extracellular electron transfer are important in advanced technologies such as those used in microbial electrochemical remediation systems (MERS) Few bacterial strains have been, nevertheless, obtained from MERS exoelectrogenic biofilms and characterized for bioremediation potential. Here we report the identification of one such bacterial strain, Citrobacter sp. KVM11, a petrophilic, iron reducing bacterial strain isolated from hydrocarbon fed MERS, producing anodic currents in microbial electrochemical systems. Fe(III) reduction of 90.01 ± 0.43% was observed during 5 weeks of incubation with Fe(III) supplemented liquid cultures. Biodegradation screening assays showed that the hydrocarbon degradation had been carried out by metabolically active cells accompanied by growth. The characteristic feature of diazo dye decolorization was used as a simple criterion for evaluating the electrochemical activity in the candidate microbe. The electrochemical activities of the strain KVM11 were characterized in a single chamber fuel cell and three electrode electrochemical cells. The inoculation of strain KVM11 amended with acetate and citrate as the sole carbon and energy sources has resulted in an increase in anodic currents (maximum current density) of 212 ± 3 and 359 ± mA/m2 with respective coulombic efficiencies of 19.5 and 34.9% in a single chamber fuel cells. Cyclic voltammetry studies showed that anaerobically grown cells of strain KVM11 are electrochemically active whereas aerobically grown cells lacked the electrochemical activity. Electrobioremediation potential of the strain KVM11 was investigated in hydrocarbonoclastic and dye detoxification conditions using MERS. About 89.60% of 400 mg l-1 azo dye was removed during the first 24 h of operation and it reached below detection limits by the end of the batch operation (60 h). Current generation and biodegradation capabilities of strain KVM11 were examined using an initial concentration of 800 mg l-1 of diesel range hydrocarbons (C9-C36) in MERS (maximum currentdensity 50.64 ± 7 mA/m2; power density 4.08 ± 2 mW/m2, 1000 ω, hydrocarbon removal 60.14 ± 0.7%). Such observations reveal the potential of electroactive biofilms in the simultaneous remediation of hydrocarbon contaminated environments with generation of energy.

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“…The ARB can conduct extracellular electron transfer via direct electron transfer (DET) and/or mediated electron transfer (MET) [Kim et al 1999], which is also held by iron-reducing bacteria (FRB), such as Shewanella and Geobacter sp., that can grow upon reduction of Fe(III) [Kostka et al 2002]. Most ARB strains were identified from anodic biofilm of an operating MFC (recent review is available in [Huang et al, 2015]. Conversely, the studies on the use of pure cultures FRB isolated from non-MFC samples as inoculum for startup of an MFC are rare [Liu et al, 2014].…”

Section: Introductionmentioning

confidence: 99%

Startup of Microbial Fuel Cells with Pure Ferric Iron-reducing Bacteria Isolates

Lee¹,

Wang²,

Ren³

et al. 2016

JEES

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Ferric iron-reducing bacteria (FRB) can transfer intercellular electrons to a surrounding solid, a function also exhibited by the anode-respiring bacteria for microbial fuel cells (MFC). This study isolated from river sediment two FRB, a Gram-negative strain (Geobaccter sp. LAR-2, GenBank No. KC211015) and a Gram-positive strain (Clostridium sp. LAR-3, GenBank No. KC211016), and individually started up MFC using these two isolates as inocula at anodic compartment. The LAR-2-MFC had an open-circuit voltage (OCV) of 610 mV and maximum power density (P max ) of 860 mW m -2 . The corresponding OCV and P max of LAR-3-MFC were 630 mV and 310 mW m -2 , respectively. The resistances of electron transfer across anodic biofilms, eletrolytes and cathodes were characterized. The tests with these two FRB support the hypothesis with FRB being effectively functioned as anode-respiring bacteria for MFC.

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Exoelectrogenic Bacterium Phylogenetically Related to Citrobacter freundii, Isolated from Anodic Biofilm of a Microbial Fuel Cell

Cited by 42 publications

References 35 publications

Comparison of Carbon Dioxide with Anaerobic Digester Biogas as a Methanogenic Biocathode Feedstock

Comparison of Carbon Dioxide with Anaerobic Digester Biogas as a Methanogenic Biocathode Feedstock

Petrophilic, Fe(III) Reducing Exoelectrogen Citrobacter sp. KVM11, Isolated From Hydrocarbon Fed Microbial Electrochemical Remediation Systems

Startup of Microbial Fuel Cells with Pure Ferric Iron-reducing Bacteria Isolates

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