Exocytotic Release of Catecholamine From Perfused Adrenal Gland of Guinea‐pig Induced by Veratridine

S, Itó; Nakazato, Yoshikazu; Ohga, Akira

doi:10.1111/j.1476-5381.1980.tb09771.x

Cited by 24 publications

(19 citation statements)

References 25 publications

(30 reference statements)

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“…of mean). There are no significant differences between these values, suggesting that these secretagogues caused an exocytotic release of catecholamine from isolated chromaffin cells in a manner similar to that from perfused adrenal gland (Ito et al 1980). Time course of catecholamine and dopamine-fi-hydroxylase secretion from isolated adrenal chromaffin cells…”

Section: Resultsmentioning

confidence: 99%

“…Although the release of DBH -is considered to be an index of the exocytotic release of catecholamine from adrenal gland (Viveros et al 1968) and sympathetic neurones (De Potter, De Schaepdryver, Moerman & Smith, 1969), it has been reported that the time course of catecholamine secretion was different from that of concomitant DBH secretion in both tissues (Cubeddu et al 1974; Dixon et al 1975;Jacobs et al 158 S. ITO 1978;Edwards et al 1980;Ito et al 1980;Muscholl et al 1980). It may be possible that delayed output of DBH, compared with catecholamine secretion, results from a delayed washout of DBH from the release site into the circulation because of the presence of diffusion barriers for this large protein.…”

Section: Resultsmentioning

confidence: 99%

“…It has, however, been reported that the time course of DBH secretion was different from that of catecholamine secretion in perfused adrenal glands of cat (Dixon, Garcia & Kirpekar, 1975), ox (Jacobs, Henry, Johnson & Williams, 1978) and guinea-pig (Ito, Nakazato & Ohga, 1980) and in adrenal gland of conscious calf (Edwards, Furness & Helle, 1980). DBH secretion from sympathetic neurones also lagged behind catecholamine secretion in perfused cat spleen (Cubeddu, Barnes, Langer & Weiner, 1974) and rabbit heart (Muscholl, Racke & Ritzel, 1980).…”

Section: Introductionmentioning

confidence: 99%

“…ATP and its metabolites were released in the effluent of perfused adrenal gland of cat (Douglas et al 1965;Douglas & Poisner, 1966a, b), ox (Banks, 1966;Lastowecka & Trifaro, 1974) and guinea-pig (Ito et al 1980). Although adenine nucleotide secretion was closely related to catecholamine secretion in cat and guinea-pig adrenal glands, most of the adenine nucleotide that appeared in the effluent was AMP but not ATP.…”

Section: Introductionmentioning

confidence: 99%

“…ADP and AMP were determined after enzymatic conversion to ATP using pyruvate kinase and pyruvate kinase plus adenylate kinase, respectively, by the methods described previously (Ito et al 1980). Perifusion medium was acidified with 1-6 N-perchloric acid giving a final concentration of 0 4 N and then centrifuged to remove protein.…”

Section: S Itomentioning

confidence: 99%

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Time course of release of catecholamine and other granular contents from perifused adrenal chromaffin cells of guinea‐pig.

Ito¹

1983

The Journal of Physiology

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SUMMARY1. Experiments were carried out to investigate the time course of the release of catecholamine, dopamine-,8-hydroxylase (DBH) and adenine nucleotides from isolated chromaffin cells of guinea-pig adrenal gland.2. When the isolated chromaffin cells were incubated with medium containing acetylcholine (ACh) (0-1 mM), veratridine (0-1 mM) or scorpion (Leiurus quinquestriatus) venom, (10 ,ug/ml.), catecholamine was released into the medium. Catecholamine secretion induced by veratridine or scorpion venom was inhibited by tetrodotoxin (1 gM) but not by atropine (0-1 mM) plus hexamethonium (041 mM). On the other hand, the secretary response to ACh was abolished by the cholinergic blocking drugs but not by tetrodotoxin.3. DBH was released together with catecholamine into the medium in which cells were suspended with these drugs. The ratio of catecholamine (n-mole) to DBH activity (n-mole/hr) appearing in the supernatant was 7-08 + 055, 6-60 + 0X27 and 8X91 + 0 47 for ACh, veratridine and scorpion venom, respectively. These values were close to that found in the lysate of chromaffin granules obtained from guinea-pig adrenal glands (7-37 + 0-39).4. The application of ACh or veratridine to perifused chromaffin cells was found to cause a parallel increase in catecholamine and DBH secretion in the perifusion medium without corresponding amounts of phenylethanolamine-N-methyltransferase leakage. However, DBH secretion tended to last for a longer period than catecholamine secretion.5. Adenine nucleotides were released from perifused chromaffin cells together with catecholamine, by ACh and veratridine. ATP added to the perifusion medium was metabolized to ADP and AMP, of which the ratio (ATP, 21-6 %; ADP, 34 %; AMP, 17-9 %) was close to those of adenine nucleotides released from the cells.6. The secretion ofadenine nucleotides induced by both secretagogues ceased much faster than the catecholamine secretion, so that the molar ratio of catecholamine to adenine nucleotides was gradually increased during and after stimulation. 7. The results indicate that catecholamine secretion is accompanied with a simultaneous release of DBH and ATP from adrenal chromaffin cells. Therefore, it is suggested that the delayed output of DBH, unlike catecholamine secretion, in perfused adrenal glands results from the presence of a diffusion barrier for this protein. The releasable secretary granules of isolated chromaffin cells are suggested to be heterogeneous with respect to the ratio of catecholamine to ATP.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: S Itomentioning

confidence: 99%

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Time course of release of catecholamine and other granular contents from perifused adrenal chromaffin cells of guinea‐pig.

Ito¹

1983

The Journal of Physiology

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Modulation by ouabain and diphenylhydantoin of veratridine-induced 22Na influx and its relation to 45Ca influx and the secretion of catecholamines in cultured bovine adrenal medullary cells

Wada

Izumi

Yanagihara

et al. 1985

Naunyn-Schmiedeberg's Arch. Pharmacol.

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The effects of ouabain and diphenylhydantoin on the secretion of catecholamines induced by veratridine were investigated in cultured bovine adrenal medullary cells with special reference to ion fluxes. Veratridine itself induced an influx of 22Na and 45Ca as well as secretion of catecholamines, which were antagonized by tetrodotoxin, a selective inhibitor of voltage dependent Na channels. The secretion of catecholamines caused by veratridine was not observed either in Na free or Ca free medium. Veratridine-induced influx of 45Ca did not occur in Na free medium, while veratridine-induced influx of 22Na occurred even in Ca free medium. Veratridine-induced influx of 22Na, 45Ca and secretion of catecholamines were all potentiated by ouabain, a potent inhibitor of Na,K-ATPase. Omission of K from the medium, a condition which suppresses the Na,K-ATPase activity, also augmented these cell responses caused by veratridine. On the contrary, diphenylhydantoin, which is known to decrease the intracellular concentration of Na, reduced the veratridine-induced influx of 22Na, 45Ca and secretion of catecholamines. The potentiating effects of ouabain on the veratridine-induced cell responses were all abolished by diphenylhydantoin. These findings imply that veratridine, ouabain and K removal as well as diphenylhydantoin modulate the intracellular accumulation of 22Na which is involved in the influx of 45Ca and the secretion of catecholamines.

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Selective inhibition by riluzole of voltage-dependent sodium channels and catecholamine secretion in adrenal chromaffin cells

Yokoo

Shiraishi

Kobayashi

et al. 1998

Naunyn-Schmiedeberg's Arch Pharmacol

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We examined the effects of riluzole, a neuroprotective drug, on voltage-dependent Na channels, nicotinic receptors, and voltage-dependent Ca channels, as well as catecholamine secretion, in comparison with those of verapamil and nicardipine, in primary cultures of bovine adrenal chromaffin cells. Riluzole inhibited veratridine-induced 22Na influx via voltage-dependent Na channels even in the presence of ouabain, an inhibitor of Na,K-ATPase. Blockade of Na channels by riluzole was concentration-dependent with an IC50 of 5.3 microM. It was associated with a similar concentration-related reduction of veratridine-induced 45Ca influx via voltage-dependent Ca channels, and of catecholamine secretion. Riluzole had no effect on 45Ca influx caused by high K, which directly activates voltage-dependent Ca channels, and on nicotine-induced 22Na influx, which passes through the nicotinic receptors. Verapamil and nicardipine attenuated 22Na influx caused by veratridine or nicotine at the same concentrations as they suppressed high K-induced 45Ca influx. The inhibitory effect of riluzole on veratridine-induced 22Na influx disappeared at high concentrations of veratridine. A potentiation of veratridine (site 2 toxin)-induced 22Na influx caused by alpha-scorpion venom (site 3 toxin), beta-scorpion venom (site 4 toxin), or brevetoxin PbTx-3 (site 5 toxin), occurred in the presence of riluzole in the same manner as in control cells. These results suggest that riluzole binds to the veratridine site in voltage-dependent Na channels. It does not impair the cooperative interaction between the functional peptide segments of Na channels, but selectively inhibits gating of Na channels, thereby reducing Ca influx via Ca channels and catecholamine secretion. In contrast, verapamil and nicardipine suppress Na influx both Na channels and nicotinic receptors.

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Exocytotic Release of Catecholamine From Perfused Adrenal Gland of Guinea‐pig Induced by Veratridine

Cited by 24 publications

References 25 publications

Time course of release of catecholamine and other granular contents from perifused adrenal chromaffin cells of guinea‐pig.

Time course of release of catecholamine and other granular contents from perifused adrenal chromaffin cells of guinea‐pig.

Modulation by ouabain and diphenylhydantoin of veratridine-induced 22Na influx and its relation to 45Ca influx and the secretion of catecholamines in cultured bovine adrenal medullary cells

Selective inhibition by riluzole of voltage-dependent sodium channels and catecholamine secretion in adrenal chromaffin cells

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