Existence of hydroxylated MWCNTs demotes the catalysis effect of amylases against starch degradation

Sekar, Gajalakshmi; Sivakumar, A.; Mukherjee, Amitava; Chandrasekaran, Natarajan

doi:10.1016/j.ijbiomac.2016.01.071

Cited by 7 publications

(1 citation statement)

References 80 publications

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“… The α-helical content reduced and unfolding started. MD, UV, CD 135 -OH Amylase The loss of the α-helical structure occurred, decreasing from 41.1% to 21.9% CD 136 None BSA The β-sheet content decreased from 33.3% to 29.8%; The β-turn content increased from 2% to 5%. CD 137 -COOH HSA, FG, IgG, histone H1 (H1) HAS, FG, and IgG showed a red shift in fluorescence, indicative of conformational changes in the hydrophobic core open, while H1 showed a blue shift.…”

Section: Conformational Changes Of Proteins In the Protein Coronamentioning

confidence: 98%

<p>Protein–Nanoparticle Interaction: Corona Formation and Conformational Changes in Proteins on Nanoparticles</p>

Park¹

2020

IJN

179

120

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Nanoparticles (NPs) are highly potent tools for the diagnosis of diseases and specific delivery of therapeutic agents. Their development and application are scientifically and industrially important. The engineering of NPs and the modulation of their in vivo behavior have been extensively studied, and significant achievements have been made in the past decades. However, in vivo applications of NPs are often limited by several difficulties, including inflammatory responses and cellular toxicity, unexpected distribution and clearance from the body, and insufficient delivery to a specific target. These unfavorable phenomena may largely be related to the in vivo protein–NP interaction, termed “protein corona.” The layer of adsorbed proteins on the surface of NPs affects the biological behavior of NPs and changes their functionality, occasionally resulting in loss-of-function or gain-of-function. The formation of a protein corona is an intricate process involving complex kinetics and dynamics between the two interacting entities. Structural changes in corona proteins have been reported in many cases after their adsorption on the surfaces of NPs that strongly influence the functions of NPs. Thus, understanding of the conformational changes and unfolding process of proteins is very important to accelerate the biomedical applications of NPs. Here, we describe several protein corona characteristics and specifically focus on the conformational fluctuations in corona proteins induced by NPs.

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Section: Conformational Changes Of Proteins In the Protein Coronamentioning

confidence: 98%

<p>Protein–Nanoparticle Interaction: Corona Formation and Conformational Changes in Proteins on Nanoparticles</p>

Park¹

2020

IJN

179

120

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Effect of mass of pristine carbon nanotubes on the photolysis of phenylalanine

Humeres

Souza

Debacher

et al. 2018

J of Physical Organic Chem

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Photolyses at 254 nm of aqueous solutions of phenylalanine (Phe) were carried out at 25°C in the absence and presence of multiwalled carbon nanotubes (CNT). The photolyses were performed in distilled, deoxygenated water and inert Ar atmosphere or in distilled water and air atmosphere. Kinetics of the photolyses were followed by UV spectrophotometry at 220 nm. Initial rates were obtained from the plot of ΔA versus Δt, both in the presence of CNT (ΔA/Δt = p*) and in their absence (ΔA/Δt = po*). The ratio p*/ po* gave the ratio k*/ ko* of the rate constants of the photolysis in the presence and in the absence of nanotubes, respectively. The mass of nanotubes produced an exponential attenuation of the photolytic decomposition of Phe with minor formation of tyrosine and an attenuation constant κ of the shielding effect of the nanotubes against the photolytic degradation of the amino acid. The results show the effects of CNT on the photolysis of Phe and support the previously observed attenuation effect of carbons on the denaturalizatoion of amino acids and proteins. It is concluded that carbons produce a shielding effect on the photolysis, absorbing the photons and decreasing substantially their denaturalization while at the same time excites the active sites of the carbon for the insertion reaction of the organic moiety.

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In vitro inhibitory effects of Chinese bayberry (Myrica rubra Sieb. et Zucc.) leaves proanthocyanidins on pancreatic α-amylase and their interaction

Wang

Chen

et al. 2020

Bioorganic Chemistry

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Existence of hydroxylated MWCNTs demotes the catalysis effect of amylases against starch degradation

Cited by 7 publications

References 80 publications

<p>Protein–Nanoparticle Interaction: Corona Formation and Conformational Changes in Proteins on Nanoparticles</p>

<p>Protein–Nanoparticle Interaction: Corona Formation and Conformational Changes in Proteins on Nanoparticles</p>

Effect of mass of pristine carbon nanotubes on the photolysis of phenylalanine

In vitro inhibitory effects of Chinese bayberry (Myrica rubra Sieb. et Zucc.) leaves proanthocyanidins on pancreatic α-amylase and their interaction

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