EXISTENCE OF a Β3-Adrenoceptor AND ITS FUNCTIONAL ROLE IN THE HUMAN URETER

Park, Young-Chol; Tomiyama, Yoshitaka; Hayakawa, Kou; Akahane, Masuo; Ajisawa, Yukiyoshi; Miyatake, Ryuichiro; Kiwamoto, Hiro; Sugiyama, Takahide; Kurita, Takashi

doi:10.1016/s0022-5347(05)67200-x

Cited by 60 publications

(22 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…While we are not aware of β 3 -adrenoceptor mRNA studies on rat or mouse ureter, its expression level in human ureter was reported to be low (Park et al 2000;Matsumoto et al 2013); moreover, in some species including humans, ureter relaxation by β 3 -adrenoceptor agonists has been reported (Park et al 2000;Matsumoto et al 2013). Several studies have reported β 3 -adrenoceptor mRNA in rat bladder, including specifically in its urothelium (Seguchi et al 1998;Fujimura et al 1999;Barendrecht et al 2009;Kullmann et al 2011).…”

Section: Discussionmentioning

confidence: 93%

Rat β3-adrenoceptor protein expression: antibody validation and distribution in rat gastrointestinal and urogenital tissues

Cernecka

Pradidarcheep

Lamers

et al. 2014

Naunyn-Schmiedeberg's Arch Pharmacol

View full text Add to dashboard Cite

β3-Adrenoceptors play important roles in the regulation of urogenital and probably gastrointestinal function. However, despite recent progress, their detection at the protein level has remained difficult due to a lack of sufficiently validated selective antibodies. Therefore, we have explored the selectivity of two antibodies for the detection of rodent β3-adrenoceptors in immunoblots and immunohistochemistry. Of two reportedly promising candidates, antibody AB15688 did not exhibit subtype selectivity in immunoblots. In contrast, the antibody Sc1473 exhibited at least some selectivity in immunoblots and more promising results in immunocytochemical and immunohistochemical stains in cells transfected with cloned β-adrenoceptor subtypes and in rat and mouse tissues. In a systematic screening of rat gastrointestinal and urogenital tissues, Sc1473 produced selective staining in the epithelial cell lining of the stomach and the urothelium of ureter and bladder. We conclude that the two tested antibodies are inappropriate or at least insufficient for immunoblotting applications, but Sc1473 appears to be useful for immunohistochemical detection of β3-adrenoceptor protein in rodent tissues. The β3-adrenoceptor protein exhibits a distinct expression pattern in the rat gastrointestinal and urogenital tract, which is at least partly in line with previously reported functional data.

show abstract

Section: Discussionmentioning

confidence: 93%

Rat β3-adrenoceptor protein expression: antibody validation and distribution in rat gastrointestinal and urogenital tissues

Cernecka

Pradidarcheep

Lamers

et al. 2014

Naunyn-Schmiedeberg's Arch Pharmacol

View full text Add to dashboard Cite

show abstract

“…CL 316,243 appears to be a rodent-selective agonist, as its potency to stimulate cAMP formation in transfected CHO cells was much higher at the mouse than at the human β 3 -adrenoceptor (pEC 50 8.7 vs. 4.3) (Hutchinson et al 2006). CL 316,243 has repeatedly been used in studies on the rat (Kaidoh et al 2002;Takeda et al 2000Takeda et al , 2002bTakeda et al , 2003Woods et al 2001) and human urinary bladder (Igawa et al 1999(Igawa et al , 2001) and also in the ureter (Murakami et al 2000;Park et al 2000), esophagus (Sarma et al 2003), colon (Kaumann and Molenaar 1996) and blood vessels (Atef et al 1996;Kuratani et al 1994;Leblais et al 2005). In line with the above differences in potency between mouse and human receptors, it also exhibited a consistently higher potency at rat compared with human tissues.…”

Section: Adrenaline and Noradrenalinementioning

confidence: 99%

Tools to study β3-adrenoceptors

Vrydag

Michel

2007

Naunyn-Schmied Arch Pharmacol

View full text Add to dashboard Cite

β 3 -adrenoceptors mediate some of the effects of catecholamines on tissues such as blood vessels or the urinary bladder and are putative targets for the treatment of diseases such as the overactive bladder syndrome. Progress in the understanding of the presence, function, and regulation of β 3 -adrenoceptors has been hampered by a lack of highly specific tools. "Classical" is not selective for β 3 -adrenoceptors, at least in humans, and may actually be a partial agonist. Radioligands, which are suitable either for the selective labeling of β 3 -adrenoceptors or for the nonselective labeling of all β-adrenoceptor subtypes, are also missing. β 3 -and β 1 /β 2 double knockout mice have been reported, but their usefulness for extrapolations in humans is questionable based upon major differences between humans and rodents with regard to the ligand recognition and expression profiles of β 3 -adrenoceptors. While the common availability of more selective agonists and antagonists at the β 3 -adrenoceptor is urgently awaited, the limitations of the currently available tools need to be considered in studies of β 3 -adrenoceptor for the time being.

show abstract

“…Ureteral access sheath also can provide protection against elevated renal pressures during routine ureteroscopic stone surgery [23]. In addition, some documents reported that some β-receptor agonist can cause relaxation of the upper urinary tract and reduce the pelvic pressure, such as β2-agonists and β3-agonists [6,9,11,12,13,14,15,18,19,24,25]. …”

Section: Discussionmentioning

confidence: 99%

“…Major experimental studies on modulating the smooth muscles in the ureter and pelvis in order to reduce pelvic pressure have been carried out in vitro and in vivo. Various pharmacological agents have shown great potential for lowering pelvic pressure, such as isoproterenol, terbutaline, norepinephrine, prostaglandin synthetase inhibitors and adrenaline [6,8,9,10,11,12,13,14,15]. In addition, a great deal of evidence has demonstrated that calcium channel blockers (CCBs) are capable of relaxing the smooth muscle of the ureter and pelvis as well as relieving ureteral colic [16,17,18,19].…”

Section: Introductionmentioning

confidence: 99%

Verapamil Inhibits the Pelvic Pressure Increase Corresponding to Flow Perfusion in the Porcine Percutaneous Renal Puncture Model

Shao¹,

Wei²,

M³

et al. 2016

Urol Int

View full text Add to dashboard Cite

Purpose: To estimate the effects of verapamil on pelvic pressure and investigate the possible systemic side effects in the porcine model during the procedure of increasing perfusion rates. Materials and Methods: In the experimental group, the pelvic pressure was recorded with increasing perfusion rates of the renal pelvis (0, 2, 4, 6, 8, 10, 14 and 20 ml/min) in response to intraluminal administration of increasing concentrations of verapamil (0, 0.1, 1, 10 and 100 µg/ml) in isotonic saline. In the isotonic saline group, the pressure flow study was also done by increasing perfusion rates of the renal pelvis per animal using isotonic saline without verapamil. Results: Perfusion with 1, 10 and 100 µg/ml verapamil caused a decrease in pelvic pressure as a response to increasing flow rates, whereas perfusion with 0.1 µg/ml verapamil did not obviously inhibit the increase of pelvic pressure at all perfusion rates compared with other concentrations. Importantly, the mean systolic blood pressure and mean heart rate were stable despite the increase of flow rates and verapamil concentrations. In the isotonic saline group, perfusion with isotonic saline resulted in an increase in the pelvic pressure with an increase in flow rates each time. Conclusions: Endoluminal administration of verapamil reduces pelvic pressure significantly without any untoward systemic cardiovascular side effects.

show abstract

EXISTENCE OF a Β3-Adrenoceptor AND ITS FUNCTIONAL ROLE IN THE HUMAN URETER

Abstract: The present results provide the first evidence that the beta3-AR subtype is distributed in human ureteral smooth muscle and that it, and beta2-AR, mediate the ureteral relaxation induced by adrenergic stimulation.

Cited by 60 publications

References 14 publications

Rat β3-adrenoceptor protein expression: antibody validation and distribution in rat gastrointestinal and urogenital tissues

Rat β3-adrenoceptor protein expression: antibody validation and distribution in rat gastrointestinal and urogenital tissues

Tools to study β3-adrenoceptors

Verapamil Inhibits the Pelvic Pressure Increase Corresponding to Flow Perfusion in the Porcine Percutaneous Renal Puncture Model

Contact Info

Product

Resources

About