Existence of a breaking point in cilia and flagella

Blum, J. J.

doi:10.1016/0022-5193(71)90065-8

Cited by 63 publications

(81 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The earliest articles describing primary cilia put forth the suggestion that ciliogenesis forced cells to become quiescent by removing the centriole from the mitotic cycle (Blum, 1971;Quarmby, 2004). Nearly a century later, quiescent fibroblasts were observed to undergo a biphasic loss of primary cilia when stimulated with growth factors (Tucker et al, 1979a,b).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Deciliation Is Associated with Dramatic Remodeling of Epithelial Cell Junctions and Surface Domains

Overgaard¹,

Sanzone²,

Spiczka³

et al. 2009

MBoC

View full text Add to dashboard Cite

Stress-induced shedding of motile cilia (autotomy) has been documented in diverse organisms and likely represents a conserved cellular reaction. However, little is known about whether primary cilia are shed from mammalian epithelial cells and what impact deciliation has on polarized cellular organization. We show that several chemically distinct agents trigger autotomy in epithelial cells. Surprisingly, deciliation is associated with a significant, but reversible increase in transepithelial resistance. This reflects substantial reductions in tight junction proteins associated with "leaky" nephron segments (e.g., claudin-2). At the same time, apical trafficking of gp80/clusterin and gp114/CEACAM becomes randomized, basal-lateral delivery of Na,K-ATPase is reduced, and expression of the nonciliary apical protein gp135/podocalyxin is greatly decreased. However, ciliogenesis-impaired MDCK cells do not undergo continual junction remodeling, and mature cilia are not required for autotomy-associated remodeling events. Deciliation and epithelial remodeling may be mechanistically linked processes, because RNAi-mediated reduction of Exocyst subunit Sec6 inhibits ciliary shedding and specifically blocks deciliation-associated down-regulation of claudin-2 and gp135. We propose that ciliary autotomy represents a signaling pathway that impacts the organization and function of polarized epithelial cells.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Ciliary resorption may also be regulated by de-acetylation of axonemal microtubules (Pugacheva et al, 2007). In contrast, deciliation is a rapid shedding of cilia in response to environmental stress (Blum, 1971;Quarmby, 2004). This may include growth factors that stimulate quiescent cells to reenter the cell cycle (Tucker et al, 1979a,b).…”

Section: Introductionmentioning

confidence: 99%

Deciliation Is Associated with Dramatic Remodeling of Epithelial Cell Junctions and Surface Domains

Overgaard¹,

Sanzone²,

Spiczka³

et al. 2009

MBoC

View full text Add to dashboard Cite

show abstract

“…The cilia and flagella of many animal and plant cells can be detached at their basal regions by various natural and artificial stimuli (2). It is likely that local anesthetics could prove useful in studying the mechanisms of detachment and regeneration in species other than the one described here.…”

Section: R E S U L T Smentioning

confidence: 99%

NONLETHAL DECILIATION OF TETRAHYMENA BY A LOCAL ANESTHETIC AND ITS UTILITY AS A TOOL FOR STUDYING CILIA REGENERATION

Thompson

Baugh

Walker

1974

The Journal of Cell Biology

102

View full text Add to dashboard Cite

“…Chlamydomonas, like other ciliated or flagellated cells, sheds its flagella in response to a variety of stimuli (Mintz and Lewin, 1954;Blum, 1971;Thompson et al, 1974;Lewin et al, 1980;Witman, 1986;Quarmby et al, 1992). This cellular behavior, known as deflagellation or flagellar autotomy, is a highly specific process that involves the severing of the nine outer doublet axonemal microtubules.…”

Section: Introductionmentioning

confidence: 99%

A Role for Katanin-mediated Axonemal Severing duringChlamydomonasDeflagellation

Lohret

McNally

Quarmby

1998

MBoC

View full text Add to dashboard Cite

Deflagellation of Chlamydomonas reinhardtii, and other flagellated and ciliated cells, is a highly specific process that involves signal-induced severing of the outer doublet microtubules at a precise site in the transition region between the axoneme and basal body. Although the machinery of deflagellation is activated by Ca2+, the mechanism of microtubule severing is unknown. Severing of singlet microtubules has been observed in vitro to be catalyzed by katanin, a heterodimeric adenosine triphosphatase that can remove tubulin subunits from the walls of stable microtubules. We found that purified katanin induced an ATP-dependent severing of theChlamydomonas axoneme. Using Western blot analysis and indirect immunofluorescence, we demonstrate thatChlamydomonas expresses a protein that is recognized by an anti-human katanin antibody and that this protein is localized, at least in part, to the basal body complex. Using an in vitro severing assay, we show that the protein(s) responsible for Ca2+-activated outer doublet severing purify with the flagellar-basal body complex. Furthermore, deflagellation of purified flagellar-basal body complexes is significantly blocked by the anti-katanin antibody. Taken together, these data suggest that a katanin-like mechanism may mediate the severing of the outer doublet microtubules during Chlamydomonas deflagellation.

show abstract

Existence of a breaking point in cilia and flagella

Cited by 63 publications

References 20 publications

Deciliation Is Associated with Dramatic Remodeling of Epithelial Cell Junctions and Surface Domains

Deciliation Is Associated with Dramatic Remodeling of Epithelial Cell Junctions and Surface Domains

NONLETHAL DECILIATION OF TETRAHYMENA BY A LOCAL ANESTHETIC AND ITS UTILITY AS A TOOL FOR STUDYING CILIA REGENERATION

A Role for Katanin-mediated Axonemal Severing duringChlamydomonasDeflagellation

Contact Info

Product

Resources

About