Existence of 2-symbol balanced arrays of strength t and t+2 constraints

Kuriki, Shinji

doi:10.1016/0378-3758(88)90126-7

Cited by 4 publications

(3 citation statements)

References 2 publications

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“…PTH-Cys(Cam), however, was eluted at t R = 4.22 min between PTH-serine (4.04 min) and PTH-threonine (4.40 min) as shown in Figure E with proper detection and identification. The observed elution pattern for PTH-Cys(Cam) is in agreement with an earlier study performed by Shimadzu researchers . To the best of our knowledge, a comparison for PTH-Cys(Me) applying the same setup was not performed so far.…”

Section: Resultssupporting

confidence: 89%

“…Similar results have been recently reported by Kuriki for the nonapeptide oxytocin that also contains one disulfide bridge which was identified by carbamidomethylation, carboxymethylation, or pyridylethylation of the reduced cysteines. , However, data on the application of PTH-cysteine standards for comparison were not given and identification of the respective PTH-cysteine derivative could only be derived from the appearance of a new peak in the chromatogram in comparison to the unmodified peptide. , …”

Section: Resultssupporting

confidence: 83%

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Edman Degradation Reveals Unequivocal Analysis of the Disulfide Connectivity in Peptides and Proteins

Elsayed,

Kühl,

Imhof

2024

Anal. Chem.

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Disulfide bridges in peptides and proteins play an essential role in maintaining their conformation, structural integrity, and consequently function. Despite ongoing efforts, it is still not possible to detect disulfide bonds and the connectivity of multiply bridged peptides directly through a simple and sufficiently validated protein sequencing or peptide mapping method. Partial or complete reduction and chemical cysteine modification are required as initial steps, followed by the application of a proper detection method. Edman degradation (ED) has been used for primary sequence determination but is largely neglected since the establishment of mass spectrometry (MS)-based protein sequencing. Here, we evaluated and thoroughly characterized the phenyl thiohydantoin (PTH) cysteine derivatives PTH-S-methyl cysteine and PTH-S-carbamidomethyl cysteine as bioanalytical standards for cysteine detection and quantification as well as for the elucidation of the disulfide connectivity in peptides by ED. Validation of the established derivatives was performed according to the guidelines of the International Committee of Harmonization on bioanalytical method validation, and their analytical properties were confirmed as reference standards. A series of model peptides was sequenced to test the usability of the PTH-Cys-derivatives as standards, whereas the native disulfide-bonded peptides CCAP-vil, μconotoxin KIIIA, and human insulin were used as case studies to determine their disulfide bond connectivity completely independent of MS analysis.

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Section: Resultssupporting

confidence: 89%

Section: Resultssupporting

confidence: 83%

Edman Degradation Reveals Unequivocal Analysis of the Disulfide Connectivity in Peptides and Proteins

Elsayed,

Kühl,

Imhof

2024

Anal. Chem.

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“…When m = t + 1, if there exists a BA of strength t, then it is an SA. However when m = t + 2, there exists a BA of strength t such that it is not always an SA (e.g., Kuriki andShirakura, 1977). For example, …”

Section: Discussionmentioning

confidence: 99%

On the Existence Conditions for Balanced Fractional $2^{m}$ Factorial Designs of Resolution $\mathrm{R}^{\ast}(\{1\}|\mathrm{\Omega}_{\ell})$ with $N<\nu_{\ell}(m)$

Hyodo

Kuwada

Yumiba

2016

IJSP

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We consider a fractional 2 m factorial design derived from a simple array (SA) such that the (ℓ + 1)-factor and higher-order interactions are assumed to be negligible, where 2ℓ ≤ m. Under these situations, if at least the main effect is estimable, then a design is said to be of resolution R * ({1}|Ω ℓ ). In this paper, we give a necessary and sufficient condition for an SA to be a balanced fractional 2 m factorial design of resolution R * ({1}|Ω ℓ ) for ℓ = 2, 3, where the number of assemblies is less than the number of non-negligible factorial effects. Such a design is concretely characterized by the suffixes of the indices of an SA.

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On existence and construction of balanced arrays

Kuriki

1993

Discrete Mathematics

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Existence of 2-symbol balanced arrays of strength t and t+2 constraints

Cited by 4 publications

References 2 publications

Edman Degradation Reveals Unequivocal Analysis of the Disulfide Connectivity in Peptides and Proteins

Edman Degradation Reveals Unequivocal Analysis of the Disulfide Connectivity in Peptides and Proteins

On the Existence Conditions for Balanced Fractional $2^{m}$ Factorial Designs of Resolution $\mathrm{R}^{\ast}(\{1\}|\mathrm{\Omega}_{\ell})$ with $N<\nu_{\ell}(m)$

On existence and construction of balanced arrays

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