The problem of prescribing Gaussian curvature on Riemann surface with conical singularity is considered. Let (Σ, β) be a closed Riemann surface with a divisor β, and K λ = K + λ, where K : Σ → R is a Hölder continuous function satisfying max Σ K = 0, K 0, and λ ∈ R. If the Euler characteristic χ(Σ, β) is negative, then by a variational method, it is proved that there exists a constant λ * > 0 such that for any λ ≤ 0, there is a unique conformal metric with the Gaussian curvature K λ ; for any λ, 0 < λ < λ * , there are at least two conformal metrics having K λ its Gaussian curvature; for λ = λ * , there is at least one conformal metric with the Gaussian curvature K λ * ; for any λ > λ * , there is no certain conformal metric having K λ its Gaussian curvature. This result is an analog of that of Ding and Liu [14], partly resembles that of Borer, Galimberti and Struwe [3], and generalizes that of Troyanov [26] in the negative case.Keywords: Prescribing Gaussian curvature, conical singularity 2010 MSC: 58E30, 53C20The Gauss-Bonnet formula leads to Σ Ke 2u dv g = Σ κdv g = 2πχ(Σ).Note that the solvability of (1) is closely related to the sign of χ(Σ). If χ(Σ) > 0, then Σ is either the projective space RP 2 or the 2-sphere S 2 . In the case of RP 2 , it was shown by Moser