Existence and expression of the untranslated first exon of aromatase mRNA in the rat brain

Yamada-Mouri, Naoko; Hirata, Shuji; Kato, Junzo

doi:10.1016/0960-0760(96)00022-2

Cited by 30 publications

(18 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the study conducted by Lanzino et al (2001) has shown that other promoters could be implicated to direct aromatase gene expression in testicular cells. In fact, our work demonstrates the use of additional promoters, and for the first time to our knowledge, three aromatase transcripts have been observed in the germ cells studied (G-PL, PS, and RS): one is derived from promoter PII, and another one is from promoter PI.f, which is the main promoter responsible for aromatase expression in the brain (Yamada-Mouri et al 1996, Kato et al 1997. Although some transcripts issuing from PI.f have been detected in the testis of monkey (Yamada-Mouri et al 1995), rat (Yamada-Mouri et al 1996), and mouse (Golovine et al 2003), it is the first time that they are described in rat germ cells; they are more expressed in PS and RS than in a mixture of spermatogonia and preleptotene spermatocytes.…”

Section: Discussionmentioning

confidence: 99%

“…The site of splicing is placed at 20 nucleotide residues after the PII initiation transcription site. This representation was realized according to the sequence published by Hickey et al (1990), Yamada-Mouri et al (1996) and Young & McPhaul (1998).…”

Section: Discussionmentioning

confidence: 99%

“…In the rat, the cyp19 gene is situated on chromosome 8 and up to now two promoters have been described: the promoter PI.f in the brain (Yamada-Mouri et al 1996) and the promoter PII (Young & McPhaul 1998) which is the main one directing aromatase gene expression in the ovary (Hinshelwood et al 1993) and the testis (Lanzino et al 2001). In the rat testis, the cellular expression of aromatase is age dependent: during the fetal and neonatal development, aromatase is expressed in Sertoli cells, whereas in the adult, aromatase has been localized in many cell types including Leydig cells, spermatocytes, spermatids, and spermatozoa (Carreau et al 2003(Carreau et al , 2006.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Three promoters PII, PI.f, and PI.tr direct the expression of aromatase (cyp19) gene in male rat germ cells

Silandre¹,

Delalande²,

Durand³

et al. 2007

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

Aromatase is the key enzyme responsible for the irreversible transformation of androgens into estrogens. It is encoded by the cyp19 gene and is expressed in the mammalian testis under the control of the proximal promoter PII. Since both somatic and germ cells contain a biologically active aromatase, we looked for the existence of other promoters that may direct the expression of aromatase in adult rat germ cells. Besides the promoter II, we have shown the presence of transcripts derived from the brain promoter PI.f in spermatogonia-preleptotene spermatocytes (G-PL), pachytene spermatocytes (PS), and round spermatids (RS). A new aromatase cDNA has been isolated by 5 0 -rapid amplification of cDNA ends that we named I.tr (testis rat). The I.tr transcripts are found in all the testicular cell populations studied with a greater expression in PS. Because of the utilization of these three promoters in the adult rat testis, we studied their putative involvement according to the age. At 10 days, aromatase expression was very low and then strongly increased between 10 and 20 days, with a preferential activation of PII and PI.tr. Transcripts coming from PI.f were found starting from 20 days onwards. The new promoter PI.tr, localized between promoters PI.f and PII, is devoid of a TATA box but contains a transcriptional initiator (Inr) and putative regulatory sequences. Therefore, the identification of the specific trans-activating factors should bring some enlightenments to understand the regulation of these three promoters in germ cells especially according to their stage of development.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Three promoters PII, PI.f, and PI.tr direct the expression of aromatase (cyp19) gene in male rat germ cells

Silandre¹,

Delalande²,

Durand³

et al. 2007

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

show abstract

“…The 5Ј-end of clone A17 (bases 1-77; Fig. 1B) was found to be the equine homolog of aromatase exon 1f, often designated as brain specific (21,(41)(42)(43). Clone A17 was designated as a promoter 1f-derived aromatase transcript.…”

Section: Characterization Of Equine Cytochromes P450arom and P45017␣ mentioning

confidence: 99%

Dual Regulation of Promoter II- and Promoter 1f-Derived Cytochrome P450 Aromatase Transcripts in Equine Granulosa Cells during Human Chorionic Gonadotropin-Induced Ovulation: A Novel Model for the Study of Aromatase Promoter Switching

Boerboom¹

1999

Endocrinology

View full text Add to dashboard Cite

Estradiol biosynthesis is a key biochemical trait of developing follicles. To study its regulation in equine follicles, the objectives of this study were to clone and determine the structure of equine cytochrome P450 aromatase (P450AROM), and characterize the regulation of P450AROM and P450 17␣-hydroxylase/C17-20 lyase (P45017␣) messenger RNAs (mRNAs) in vivo in equine preovulatory follicles isolated during hCGinduced ovulation. Two distinct P450AROM complementary DNAs (cDNAs) were isolated from an equine preovulatory follicle cDNA library. One clone was 2682 bp in length and included 115 bp of 5Ј-untranslated region (UTR), 1509 bp of open reading frame encoding a well conserved 503-amino acid protein, and 1058 bp of 3Ј-UTR. Its 5Ј-most region represented the equine homolog of exon 1f, previously designated brain specific. The other cDNA clone encoded a truncated protein and contained a distinct 5Ј-UTR characteristic of transcripts derived from promoter II, previously identified as the predominant ovarian mRNA. Northern blot analyses were performed using preovulatory follicles obtained during estrus between 0 -39 h after the administration of hCG and with corpora lutea isolated on day 8 of the estrous cycle (day 0 ϭ day of ovulation). The results showed a biphasic regulation of P450AROM mRNA expression: levels were highest in follicles at 0 h post-hCG, decreased significantly during the ovulatory process at 12 and 24 h (P Ͻ 0.05), and increased again between 30 -39 h post-hCG and in corpora lutea. When oligonucleotides specific for P450AROM mRNA variants were used as probes, a novel switching phenomenon was observed. Promoter II-derived transcripts accounted for the message present in follicles at 0 h post-hCG and in corpora lutea, whereas promoter 1f-derived mRNA was expressed exclusively during the ovulatory process (30 -39 h post-hCG). Levels of P45017␣ mRNA were high in follicles at 0 h, but significantly decreased after hCG treatment (P Ͻ 0.05), with lowest levels in follicles at 36 and 39 h post-hCG and in corpora lutea. Northern blots performed on isolated cellular preparations revealed that P450AROM and P45017␣ transcripts were localized exclusively in granulosa cells and theca interna, respectively. Equine aromatase promoters II and 1f were cloned from a genomic library, and putative transcription start sites were characterized by primer extension assays. Sequence analyses identified distinct potential regulatory elements in each promoter. Thus, this study identifies a novel aromatase promoter-switching phenomenon in equine granulosa cells during follicular luteinization and provides a new model in which aromatase promoter switching is induced in vivo. (Endocrinology 140: 4133-4141, 1999)

show abstract

“…In rat, the CYP19 gene is situated on chromosome 8 and up to now three promoters have been described: the promoter PI.f used in brain (Yamada-Mouri et al 1996) but also in testis (Silandre et al 2007), the promoter PII (Young & McPhaul 1998), which is the main one directing aromatase gene expression in gonads (Lanzino et al 2001;Pezzi et al 2004), and the promoter PI.tr only used in testis (Silandre et al 2007). Indeed, in figure 1, we have provided a schematic presentation of these promoters used according to age in the rat gonad, which suggests a very precise control of the expression of aromatase and the amount of oestrogens produced.…”

Section: Oestrogen Sources In Mammalian Testicular Cells (A) Cyp19 Genementioning

confidence: 99%

Oestrogens and spermatogenesis

Carreau

Hess

2010

Phil. Trans. R. Soc. B

263

220

View full text Add to dashboard Cite

The role of oestrogens in male reproductive tract physiology has for a long time been a subject of debate. The testis produces significant amounts of oestrogenic hormones, via aromatase, and oestrogen receptors (ERs)a (ESR1) and ERb (ESR2) are selectively expressed in cells of the testis as well as the epididymal epithelium, depending upon species. This review summarizes the current knowledge concerning the presence and activity of aromatase and ERs in testis and sperm and the potential roles that oestrogens may have in mammalian spermatogenesis. Data show that physiology of the male gonad is in part under the control of a balance of androgens and oestrogens, with aromatase serving as a modulator.

show abstract

Existence and expression of the untranslated first exon of aromatase mRNA in the rat brain

Cited by 30 publications

References 17 publications

Three promoters PII, PI.f, and PI.tr direct the expression of aromatase (cyp19) gene in male rat germ cells

Three promoters PII, PI.f, and PI.tr direct the expression of aromatase (cyp19) gene in male rat germ cells

Dual Regulation of Promoter II- and Promoter 1f-Derived Cytochrome P450 Aromatase Transcripts in Equine Granulosa Cells during Human Chorionic Gonadotropin-Induced Ovulation: A Novel Model for the Study of Aromatase Promoter Switching

Oestrogens and spermatogenesis

Contact Info

Product

Resources

About