Existence and characterization of Salsolinol synthase in neuronal cells and rat brain

Chen, X. C.; Chen, Y.; Wu, Guangxiang; Lu, Jianqing; Iqbal, Jamshed; Qing, Hong; Deng, Yulin

doi:10.1134/s1819712413030045

Cited by 6 publications

(4 citation statements)

References 23 publications

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“…Then, we used dialysis to remove the small-molecular compounds of this fraction through a selective diffusion of dissolved particles by 0.5 kDa semipermeable membranes. Figure showed that almost 75% of the Sal synthase activity was retained after dialysis, precluding the query that Sal synthase maybe one kind of small-molecular compound in our previous report . Within that paper, we also suspected that the molecular mass of Sal synthase maybe less than 3 kDa, as most of its activity exists in P3 fraction.…”

Section: Resultsmentioning

confidence: 71%

“…For a long time, the existence of Sal synthase is doubtful because neither has it been isolated nor has its amino acid sequence been determined . In the previous study, we have confirmed the presence of Sal synthase in rat brain, but it is still not fully understood. In 1996, Naoi et al had reported the preliminary isolation of the enzyme without identification .…”

Section: Introductionmentioning

confidence: 74%

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Isolation and Sequencing of Salsolinol Synthase, an Enzyme Catalyzing Salsolinol Biosynthesis

Chen

Zheng

Ali

et al. 2018

ACS Chem. Neurosci.

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Salsolinol (1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline), a derivate of dopamine, is suspected to be the most probable neurotoxin in the degeneration of dopaminergic neurons. Numerous hypotheses regarding its pathophysiological roles have been raised, especially related to Parkinson's disease and alcohol addiction. In the mammalian brain, salsolinol may be enzymatically synthesized by salsolinol synthase from dopamine and acetaldehyde. However, the direct evidence of its biosynthesis was still missing. In this study, we purified salsolinol synthase from rat brain by a systematical procedure involving acid precipitation, ultrafiltration, and hydrophilic interaction chromatography. The molecular weight of salsolinol synthase determined by MALDI-TOF MS is 8622.29 Da, comprising 77 amino acids (MQIFVKTLTG KTITLEVEPS DTIKNVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKKSTLHLV LRLRVDY). Homology analysis showed that the enzyme is a ubiquitin-like protein, with a difference of four amino acids, which suggests it is a novel protein. After it was overexpressed in eukaryotic cells, the production of salsolinol was significantly increased as compared with control, confirming the catalytic function of this enzyme. To our knowledge, it is the first systematic purification and sequencing of salsolinol synthase. Together, this work reveals a formerly anonymous protein and urges further exploration of its possible prognostic value and implications in Parkinson's disease and other related disorders.

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Section: Resultsmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 74%

Isolation and Sequencing of Salsolinol Synthase, an Enzyme Catalyzing Salsolinol Biosynthesis

Chen

Zheng

Ali

et al. 2018

ACS Chem. Neurosci.

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“…However, it is still not clear that how these CTIQs perform their neurotoxic role in PD patients. According to the information in part one, the groups of CTIQs can be biosynthesized in human brain by the certain synthetase, which has been evidenced by the fact that Deng's team confirmed the existence of Sal synthetase [22].…”

Section: Ctiqs and α-Syn Aggregationmentioning

confidence: 98%

“…However, the exact pathogenesis mechanism of this vicious cycle in PD is still not clear. Recently, Deng's group confirmed the existence of Sal synthetase, that is a thermostable enzyme, and the higher enzymatic condensation reaction of DA and acetaldehyde was detected in the striatum and substantia nigra of rat brains [22,23]. Of CTIQs, Sal is a representative neurotoxin, which presents a high level in brains of PD patients and has been shown to increase ROS, inhibit mitochondrial function and cell injury [24][25][26].…”

Section: Endogenous Catechol Tetrahydroisoquinolinesmentioning

confidence: 99%

Catechol Tetrahydroisoquinolines Enhance a-Synuclein Aggregation and Specify the Neurotoxicity to Dopaminergic Neurons

Chen¹,

Lu²,

Duan³

et al. 2017

J Alzheimers Dis Parkinsonism

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90 years of monoamine oxidase: some progress and some confusion

Tipton

2018

J Neural Transm

105

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It would not be practical to attempt to deal with all the advances that have informed our understanding of the behavior and functions of this enzyme over the past 90 years. This account concentrates key advances that explain why the monoamine oxidases remain of pharmacological and biochemical interest and on some areas of continuing uncertainty. Some issues that remain to be understood or are in need of further clarification are highlighted.

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Existence and characterization of Salsolinol synthase in neuronal cells and rat brain

Cited by 6 publications

References 23 publications

Isolation and Sequencing of Salsolinol Synthase, an Enzyme Catalyzing Salsolinol Biosynthesis

Isolation and Sequencing of Salsolinol Synthase, an Enzyme Catalyzing Salsolinol Biosynthesis

Catechol Tetrahydroisoquinolines Enhance a-Synuclein Aggregation and Specify the Neurotoxicity to Dopaminergic Neurons

90 years of monoamine oxidase: some progress and some confusion

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