Exhaled eicosanoid profiles in children with atopic asthma and healthy controls

Głowacka, Edyta; Jedynak-Wąsowicz, Urszula; Sanak, Marek; Lis, Grzegorz

doi:10.1002/ppul.22615

Cited by 16 publications

(10 citation statements)

References 42 publications

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“…Recently introduced methods, such as 2-dimensional protein gel electrophoresis (2D PEG) [ 57 ], followed by chromatographic proteomic microanalysis, are able to provide both qualitative and quantitative EBC-analysis. Contemporary chromatographic methods include gas chromatography (GC), high-performance liquid chromatography (HPLC), mass spectrometry (MS), and combinations of the abovementioned techniques, including LC-MS, GC-MS [ 58 ], HPLC-MS [ 59 ], Chromatography-Differential Mobility Spectrometry (GC-DMS) [ 60 ], and Electrospray Ionization-Differential Mobility Spectrometry (ESI-DMS) [ 61 ].…”

Section: Methodology Of Ebc Collection and Analysismentioning

confidence: 99%

Exhaled Breath Condensate: Technical and Diagnostic Aspects

Konstantinidi

Lappas

Tzortzi

et al. 2015

The Scientific World Journal

118

109

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Purpose. The aim of this study was to evaluate the 30-year progress of research on exhaled breath condensate in a disease-based approach. Methods. We searched PubMed/Medline, ScienceDirect, and Google Scholar using the following keywords: exhaled breath condensate (EBC), biomarkers, pH, asthma, gastroesophageal reflux (GERD), smoking, COPD, lung cancer, NSCLC, mechanical ventilation, cystic fibrosis, pulmonary arterial hypertension (PAH), idiopathic pulmonary fibrosis, interstitial lung diseases, obstructive sleep apnea (OSA), and drugs. Results. We found 12600 related articles in total in Google Scholar, 1807 in ScienceDirect, and 1081 in PubMed/Medline, published from 1980 to October 2014. 228 original investigation and review articles were eligible. Conclusions. There is rapidly increasing number of innovative articles, covering all the areas of modern respiratory medicine and expanding EBC potential clinical applications to other fields of internal medicine. However, the majority of published papers represent the results of small-scale studies and thus current knowledge must be further evaluated in large cohorts. In regard to the potential clinical use of EBC-analysis, several limitations must be pointed out, including poor reproducibility of biomarkers and absence of large surveys towards determination of reference-normal values. In conclusion, contemporary EBC-analysis is an intriguing achievement, but still in early stage when it comes to its application in clinical practice.

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Section: Methodology Of Ebc Collection and Analysismentioning

confidence: 99%

Exhaled Breath Condensate: Technical and Diagnostic Aspects

Konstantinidi

Lappas

Tzortzi

et al. 2015

The Scientific World Journal

118

109

View full text Add to dashboard Cite

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“…Likewise, increased levels of cysteinyl leukotrienes, which are powerful broncho-constrictors and pro-inflammatory mediators, have been reported in both children and adults, with the highest concentrations found in subjects with severe asthma, despite ongoing ICS therapy. 34 Several markers of oxidative stress have also been studied in EBC, pointing to an increased oxidative burst in asthmatic airways. 33 Interestingly, the simultaneous assessment of a set of metabolites of arachidonic acid led to the identification of a profile of eicosanoids (including prostaglandins and leukotrienes) capable of discriminating asthmatic and healthy children with high accuracy.…”

Section: Markers In Breath Condensatementioning

confidence: 99%

“…33 Interestingly, the simultaneous assessment of a set of metabolites of arachidonic acid led to the identification of a profile of eicosanoids (including prostaglandins and leukotrienes) capable of discriminating asthmatic and healthy children with high accuracy. 34 Several markers of oxidative stress have also been studied in EBC, pointing to an increased oxidative burst in asthmatic airways.…”

Section: Markers In Breath Condensatementioning

confidence: 99%

Priorities for future research into asthma diagnostic tools: A PAN‐EU consensus exercise from the European asthma research innovation partnership (EARIP)

García‐Marcos

Edwards

Kennington

et al. 2018

Clin Experimental Allergy

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The diagnosis of asthma is currently based on clinical history, physical examination and lung function, and to date, there are no accurate objective tests either to confirm the diagnosis or to discriminate between different types of asthma. This consensus exercise reviews the state of the art in asthma diagnosis to identify opportunities for future investment based on the likelihood of their successful development, potential for widespread adoption and their perceived impact on asthma patients. Using a two-stage e-Delphi process and a summarizing workshop, a group of European asthma experts including health professionals, researchers, people with asthma and industry representatives ranked the potential impact of research investment in each technique or tool for asthma diagnosis and monitoring. After a systematic review of the literature, 21 statements were extracted and were subject of the two-stage Delphi process. Eleven statements were scored 3 or more and were further discussed and ranked in a face-to-face workshop. The three most important diagnostic/predictive tools ranked were as follows: "New biological markers of asthma (eg genomics, proteomics and metabolomics) as a tool for diagnosis and/or monitoring," "Prediction of future asthma in preschool children with reasonable accuracy" and "Tools to measure volatile organic compounds (VOCs) in exhaled breath."

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“…67,68 Prostaglandins and leukotrienes, both of which are arachidonic acid metabolites, have been quantified in exhaled breath condensates from children with asthma. 69 However, the extent to which asthma symptomatology and pulmonary function improve with increased intake of omega-3 or decreased intake of omega-6 fatty acids is not well known among obese children with asthma and is being investigated in ongoing randomized trials of omega-3 fatty acids supplementation. 70 Furthermore, intake of micronutrients such as vitamins A, 59, 71, 72 C, 59, 73 and E 73 has been inversely associated with asthma, whereas vitamin D insufficiency has been associated with higher asthma disease burden 74 and lower lung function.…”

Section: Diet and Asthmamentioning

confidence: 99%

Pediatric Obesity-Related Asthma: The Role of Metabolic Dysregulation

2016

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The burden of obesity-related asthma among children, particularly among ethnic minorities, necessitates an improved understanding of the underlying disease mechanisms. Although obesity is an independent risk factor for asthma, not all obese children develop asthma. Several recent studies have elucidated mechanisms, including the role of diet, sedentary lifestyle, mechanical fat load, and adiposity-mediated inflammation that may underlie the obese asthma pathophysiology. Here, we review these recent studies and emerging scientific evidence that suggest metabolic dysregulation may play a role in pediatric obesity-related asthma. We also review the genetic and epigenetic factors that may underlie susceptibility to metabolic dysregulation and associated pulmonary morbidity among children. Lastly, we identify knowledge gaps that need further exploration to better define pathways that will allow development of primary preventive strategies for obesity-related asthma in children.

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Exhaled eicosanoid profiles in children with atopic asthma and healthy controls

Abstract: Complementary measurements of PGEM, PGD2, LTC4, and 5HETE in small-volume EBC samples are feasible by HPLC-MS2 and showed a specific profile in our study population. EACR should be evaluated further in the context of diagnosing and monitoring childhood asthma.

Cited by 16 publications

References 42 publications

Exhaled Breath Condensate: Technical and Diagnostic Aspects

Exhaled Breath Condensate: Technical and Diagnostic Aspects

Priorities for future research into asthma diagnostic tools: A PAN‐EU consensus exercise from the European asthma research innovation partnership (EARIP)

Pediatric Obesity-Related Asthma: The Role of Metabolic Dysregulation

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