Exhaled Breath Condensate: Standardized Collection of Samples From Healthy Volunteers

Lema, J. Bruno de; González, Marco; Vigil, Laura; Casán, Pere

doi:10.1016/s1579-2129(06)60287-5

Cited by 13 publications

(14 citation statements)

References 5 publications

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“…The mean volume of condensate in exhaled air over a period of 15 minutes was 1.8 ± 0.5 mL (95% confidence interval, 1.5–2 mL) and the coefficient of variation was 29%. The direct relation between the volume of EBC collected and the volume of air inhaled for each individual is expressed by the following equation: volume of EBC (mL) = 0.013 × volume of air (L) + 0.255 [59]. Direct comparison of different collection times has only been studied with respect to pH: changes in collection time did not show any effect.…”

Section: Ebc Sample Collectionmentioning

confidence: 99%

Isoprostanes-Biomarkers of Lipid Peroxidation: Their Utility in Evaluating Oxidative Stress and Analysis

Janicka

Kot‐Wasik

Kot

et al. 2010

IJMS

101

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Isoprostanes (IsoPs) are key biomarkers for investigating the role of free radical generation in the pathogenesis of human disorders. To solve IsoPs-related problems with regard to isoprostanes, analytical tools are required. This paper reviews the problems and trends in this field focusing on the methodology for assaying biomarkers in exhaled breath condensate (EBC) samples. A large amount of work has been done in the qualitative and quantitative analysis of IsoPs, but a standardized method has yet to emerge. The methodologies described differ, either in the sample preparation steps or in the detection techniques, or both. Requiring a number of chromatographic steps, the relevant extraction and purification procedures are often critical and time-consuming, and they lead to a substantial loss of target compounds. Recent data show that EBC is a promising non-invasive tool for the evaluation of different diseases. Two main analytical approaches have been adopted for IsoPs measurement: immunological methods and mass spectrometry. The methodologies for the extraction, purification and analysis of IsoPs in EBC samples are presented.

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Section: Ebc Sample Collectionmentioning

confidence: 99%

Isoprostanes-Biomarkers of Lipid Peroxidation: Their Utility in Evaluating Oxidative Stress and Analysis

Janicka

Kot‐Wasik

Kot

et al. 2010

IJMS

101

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“…A number of parameters that affect composition of exhaled breath were elucidated earlier, including: EBC sampling period, breathing pattern (tidal versus forced capacity), collected fraction of the exhaled breath (alveolar end tidal versus total expired volume), collection device material and design, condensation temperature, passage of exhaled droplets and saliva trapping, sample transfer and storage [17]. These parameters still require optimization for the sample collection procedure [17, 18]. There is a great potential in optimized hardware and methods because they can help establish standard procedures and allow data comparison from different case studies.…”

Section: Introductionmentioning

confidence: 99%

Human breath metabolomics using an optimized non-invasive exhaled breath condensate sampler

et al. 2016

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Exhaled breath condensate (EBC) analysis is a developing field with tremendous promise to advance personalized, non-invasive health diagnostics as new analytical instrumentation platforms and detection methods are developed. Multiple commercially-available and researcher-built experimental samplers are reported in the literature. However, there is very limited information available to determine an effective breath sampling approach, especially regarding the dependence of breath sample metabolomic content on the collection device design and sampling methodology. This lack of an optimal standard procedure results in a range of reported results that are sometimes contradictory. Here, we present a design of a portable human EBC sampler optimized for collection and preservation of the rich metabolomic content of breath. The performance of the engineered device is compared to two commercially available breath collection devices: the RTube™ and TurboDECCS. A number of design and performance parameters are considered, including: condenser temperature stability during sampling, collection efficiency, condenser material choice, and saliva contamination in the collected breath samples. The significance of the biological content of breath samples, collected with each device, is evaluated with a set of mass spectrometry methods and was the primary factor for evaluating device performance. The design includes an adjustable mass-size threshold for aerodynamic filtering of saliva droplets from the breath flow. Engineering an inexpensive device that allows efficient collection of metalomic-rich breath samples is intended to aid further advancement in the field of breath analysis for non-invasive health diagnostic. EBC sampling from human volunteers was performed under UC Davis IRB protocol 63701-3 (09/30/2014-07/07/2017).

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“…In the present study, we ensured acceptable EBC volume by determining collection time, a strategy that is considered to be valid (16,33). However, we noted that in intubated and mechanically-ventilated patients, it takes more time to collect the same volume of condensate compared to non-intubated and normally breathing individuals (34). This may be due to the trend towards lower TVs in mechanical ventilation such that EBC collection volumes may be lower due to decreased minute ventilation.…”

Section: Discussionmentioning

confidence: 77%

Exhaled Breath Condensate Acidification Occurs During Surgery for Abdominal Cancer

Mahairidou

Rodopoulou

Tomos

et al. 2017

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Exhaled Breath Condensate: Standardized Collection of Samples From Healthy Volunteers

Cited by 13 publications

References 5 publications

Isoprostanes-Biomarkers of Lipid Peroxidation: Their Utility in Evaluating Oxidative Stress and Analysis

Isoprostanes-Biomarkers of Lipid Peroxidation: Their Utility in Evaluating Oxidative Stress and Analysis

Human breath metabolomics using an optimized non-invasive exhaled breath condensate sampler

Exhaled Breath Condensate Acidification Occurs During Surgery for Abdominal Cancer

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