Exhaled breath condensate analysis from intubated newborns by nano-HPLC coupled to high resolution MS

Кононихин, А. С.; Стародубцева, Н. Л.; Chagovets, Vitaliy; Ryndin, A. Yu.; Burov, A.; Popov, Igor; Бугрова, А. Е.; Dautov, R.A.; Tokareva, Alisa; Podurovskaya, Yulia; Ionov, Ionov O.V.; Frankevich, Vladimir; Nikolaev, Eugene; Сухих, Г. Т.

doi:10.1016/j.jchromb.2016.12.036

Cited by 24 publications

(8 citation statements)

References 60 publications

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“…The separation was carried out by a 120 min gradient (H 2 O/acetonitrile (ACN) containing 0.1% formic acid (FA)) from 3% to 50% of ACN at a flow rate of 300 nL/min. Mass spectrometry (MS) analysis was carried out by 7 T LTQ-FT Ultra (Thermo Electron, Bremen, Germany) using a nanospray ion source (positive high voltage, 2.1 kV) [30].…”

Section: Methodsmentioning

confidence: 99%

Effect of MSCs and MSC-Derived Extracellular Vesicles on Human Blood Coagulation

Silachev

Goryunov

Shpilyuk

et al. 2019

Cells

106

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Mesenchymal stem cells (MSCs) have emerged as a potent therapeutic tool for the treatment of a number of pathologies, including immune pathologies. However, unwelcome effects of MSCs on blood coagulation have been reported, motivating us to explore the thrombotic properties of human MSCs from the umbilical cord. We revealed strong procoagulant effects of MSCs on human blood and platelet-free plasma using rotational thromboelastometry and thrombodynamic tests. A similar potentiation of clotting was demonstrated for MSC-derived extracellular vesicles (EVs). To offer approaches to avoid unwanted effects, we studied the impact of a heparin supplement on MSC procoagulative properties. However, MSCs still retained procoagulant activity toward blood from children receiving a therapeutic dose of unfractionated heparin. An analysis of the mechanisms responsible for the procoagulant effect of MSCs/EVs revealed the presence of tissue factor and other proteins involved in coagulation-associated pathways. Also, we found that some MSCs and EVs were positive for annexin V, which implies the presence of phosphatidylserine on their surfaces, which can potentiate clot formation. Thus, we revealed procoagulant activity of MSCs/EVs associated with the presence of phosphatidylserine and tissue factor, which requires further analysis to avoid adverse effects of MSC therapy in patients with a risk of thrombosis.

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Section: Methodsmentioning

confidence: 99%

Effect of MSCs and MSC-Derived Extracellular Vesicles on Human Blood Coagulation

Silachev

Goryunov

Shpilyuk

et al. 2019

Cells

106

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“…Peptides were isolated by gel filtration and analyzed by high-performance liquid chromatography with tandem mass-spectrometry (HPLC-MS/MS) in full accordance with the protocols developed by the authors earlier [13,14,16]. A total of 1.5 mL of urine was mixed with 1.5 mL of denaturing buffer (4M urea, 20 mM NH4OH, 0.2% sodium dodecyl sulfate), the stages of ultrafiltration and gel filtration were carried out in order to separate proteins, purify low molecular weight contaminants, and change the buffer.…”

Section: Lc-ms/ms Analysismentioning

confidence: 99%

“…Three potential urinary PE biomarkers were found including serotransferrin (TF), complement factor B (CFB), and serum paraoxonase/arylesterase 1 (PON1) by 2-d LC-MS/MS with ELISA validation [3]. Another promising approach is based on a peptidome analysis of the biological fluids of women with PE [10][11][12][13][14]. Peptide analysis of serum samples revealed to panel of peptides, including peptides of fibrinogen alpha (FGA), α1-antitrypsin (A1AT), apolipoprotein L1 (APO-L1), inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), kininogen-1 (KNG1), and thymosin beta-4 (TMSB4), that can be used for PE diagnosis [12].…”

Section: Introductionmentioning

confidence: 99%

SERPINA1 Peptides in Urine as A Potential Marker of Preeclampsia Severity

Стародубцева

Nizyaeva

Baev

et al. 2020

IJMS

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Preeclampsia (PE) is a multisystem disorder associated with pregnancy and its frequency varies from 5 to 20 percent of pregnancies. Although a number of preeclampsia studies have been carried out, there is no consensus about disease etiology and pathogenesis so far. Peptides of SERPINA1 (α1-antitrypsin) in urine remain one of the most promising peptide markers of PE. In this study the diagnostic potential of urinary α1-antitrypsin peptides in PE was evaluated. The urinary peptidome composition of 79 pregnant women with preeclampsia (PE), chronic arterial hypertension (CAH), and a control group was investigated. Mann–Whitney U-test (p < 0.05) revealed seven PE specific SERPINA1 peptides demonstrating 52% sensitivity and 100% specificity. SERPINA1 in urine has been associated with the most severe forms of preeclampsia (p = 0.014), in terms of systolic hypertension (p = 0.01) and proteinuria (p = 0.006). According to Spearman correlation analysis, the normalized intensity of SERPINA1 urinary peptides has a similar diagnostic pattern with known diagnostic PE markers, such as sFLT/PLGF. SERPINA1 peptides were not urinary excreted in superimposed PE (PE with CAH), which is a milder form of PE. An increase in expression of SERPINA1 in the structural elements of the placenta during preeclampsia reflects a protective mechanism against hypoxia. Increased synthesis of SERPINA1 in the trophoblast leads to protein accumulation in fibrinoid deposits. It may block syncytial knots and placenta villi, decreasing trophoblast invasion. Excretion of PE specific SERPINA1 peptides is associated with syncytiotrophoblast membrane destruction degradation and increased SERPINA1 staining. It confirms that the placenta could be the origin of SERPINA1 peptides in urine. Significant correlation (p < 0.05) of SERPINA1 expression in syncytiotrophoblast membrane and cytoplasm with the main clinical parameters of severe PE proves the role of SERPINA1 in PE pathogenesis. Estimation of SERPINA1 peptides in urine can be used as a diagnostic test of the severity of the condition to determine further treatment, particularly the need for urgent surgical delivery.

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“…Application has been reported in metabolomics analysis of complex samples such as plasma, tissue, urine, breath, sweat, cerebrospinal fluids and cell extracts. 49,50,52,[80][81][82][83][84][85][86] Deviation in back pressure and surface tension due to variability in mobile phase viscosities can create challenges in obtaining stable analyte retention times, especially with gradient separations. 87,88 Direct flow is not as high-throughput as direct infusion, however, direct flow does provide increased resolution via pre-mass spectrometry separations without waste inherent with sample split-flow.…”

Section: Mass Spectrometry Data Acquisitionmentioning

confidence: 99%

Single-platform ‘multi-omic’ profiling: unified mass spectrometry and computational workflows for integrative proteomics–metabolomics analysis

2018

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The objective of omics studies is to globally measure the different classes of cellular biomolecules present in a biological specimen (e.g. proteins, metabolites) as accurately as possible in order to investigate the corresponding 'states' of biological systems. High throughput omics technologies are emerging as an increasingly powerful toolkit in the rapidly advancing field of systems biology, enabling the systematic study of dynamic molecular processes that drive core cell functions like growth, sensing, and environmental adaptation. Advances in high resolution mass spectrometry, in particular, now allow for the near comprehensive study of cellular proteins and metabolites that underlie physiological homeostasis and disease pathogenesis. Yet while the expression levels, modification states, and functional associations of diverse molecular species are now measurable, existing proteomic and metabolomic data generation and analysis workflows are often specialized and incompatible. Hence, while there are now many reports of ad hoc combinations of unimolecular proteomic and metabolomic workflows, only a limited number of multi-omic profiling approaches have been reported for obtaining different molecular measurements (proteins, metabolites, nucleic acids) in parallel from a single biological sample. Moreover, elucidating how the myriad of measured cellular components are linked together functionally within the metabolic processes, signal transduction pathways, and macromolecular interaction networks central to living systems remains a massive, complicated, and uncertain endeavor. Presented here is a review of convergent mass spectrometry-based multi-omic methodologies, with a focus on notable recent advances and remaining challenges in terms of efficient sample preparation, biochemical separations, data acquisition, and integrative computational strategies. We outline a unifying network-based integrative framework to better derive biological knowledge from integrated profiling studies with the goal of realizing the full potential of multi-omic data sets.

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Exhaled breath condensate analysis from intubated newborns by nano-HPLC coupled to high resolution MS

Cited by 24 publications

References 60 publications

Effect of MSCs and MSC-Derived Extracellular Vesicles on Human Blood Coagulation

Effect of MSCs and MSC-Derived Extracellular Vesicles on Human Blood Coagulation

SERPINA1 Peptides in Urine as A Potential Marker of Preeclampsia Severity

Single-platform ‘multi-omic’ profiling: unified mass spectrometry and computational workflows for integrative proteomics–metabolomics analysis

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