Exhaled breath concentrations of acetic acid vapour in gastro-esophageal reflux disease

Dryahina, Kseniya; Pospíšilová, Veronika; Sovová, Kristýna; Shestivská, Violetta; Kubišta, Jiřı́; Spesyvyi, Anatolii; Pehal, František; Turzíková, Jarmila; Votruba, Jiří; Španěl, Patrik

doi:10.1088/1752-7155/8/3/037109

Cited by 44 publications

(31 citation statements)

References 22 publications

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“…However, these concentrations are several times greater than endexpiratory concentration of acetic acid established for the healthy population (median 48 ppbv, range 20-60 ppbv, see Figure 2). [23]. It is pertinent to note that the concentrations of acetic acid present in the ambient air were routinely checked by SIFT-MS analyses of the room air and no measurable background was observed.…”

Section: Resultsmentioning

confidence: 99%

“…More relevant to the present results is a previous concerted study to identify potential biomarkers in the breath of patients suffering from gastro-oesophageal reflux disease (GERD) using both SIFT-MS and GC-MS analysis in parallel to ensure positive identification of VOCs [23]. End-expiratory concentration of acetic acid measured by SIFT-MS in mouth exhaled breath of 22 GERD patients (median 85 ppbv, see Figure 2) was found to be significantly higher than that in breath of a control group of 24 healthy persons (median 48 ppbv).…”

Section: Discussionmentioning

confidence: 99%

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Breath concentration of acetic acid vapour is elevated in patients with cystic fibrosis

Smith¹,

Sovová²,

Dryahina³

et al. 2016

J. Breath Res.

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A study has been carried out of the volatile organic compounds present in the exhaled breath of 58 cystic fibrosis (CF) patients. An important observation is that the acetic acid vapour concentration measured by selected ion flow tube mass spectrometry, SIFT-MS, is significantly elevated in the exhaled breath of CF patients, independent of the Pseudomonas aeruginosa (PA) infection status (PA-infected median 170 ppbv; PA-negative median 182 ppbv), compared to that of healthy controls (median 48 ppbv). The cause for this may be decreased pH of the mucus lining the CF airways. Thus, we speculate that non-invasive measurement of breath acetic acid concentration could serve as an indicator of the acidity of the CF airways mucosa.2

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Breath concentration of acetic acid vapour is elevated in patients with cystic fibrosis

Smith¹,

Sovová²,

Dryahina³

et al. 2016

J. Breath Res.

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“…Acetic acid is a common VOC resulting from normal human biochemistry (e.g. ethanol metabolism, Krebs cycle, or pyruvate metabolism) and hence released from the body via breath [38,39]. It can also be produced by cutaneous bacteria during the biotransformation of longer chain fatty acids and glycerol present in human sebum [26,40].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Monitoring of selected skin- and breath-borne volatile organic compounds emitted from the human body using gas chromatography ion mobility spectrometry (GC-IMS)

Mochalski

Wiesenhofer

Allers

et al. 2018

Journal of Chromatography B

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Human smuggling and associated cross-border crimes have evolved as a major challenge for the European Union in recent years. Of particular concern is the increasing trend of smuggling migrants hidden inside shipping containers or trucks. Therefore, there is a growing demand for portable security devices for the non-intrusive and rapid monitoring of containers to detect people hiding inside. In this context, chemical analysis of volatiles organic compounds (VOCs) emitted from the human body is proposed as a locating tool. In the present study, an in-house made ion mobility spectrometer coupled with gas chromatography (GC-IMS) was used to monitor the volatile moieties released from the human body under conditions that mimic entrapment. A total of 17 omnipresent volatile compounds were identified and quantified from 35 ion mobility peaks corresponding to human presence. These are 7 aldehydes (acrolein, 2-methylpropanal, 3-methylbutanal, 2-ethacrolein, n-hexanal, n-heptanal, benzaldehyde), 3 ketones (acetone, 2-pentanone, 4-methyl-2-pentanone), 5 esters (ethyl formate, ethyl propionate, vinyl butyrate, butyl acetate, ethyl isovalerate), one alcohol (2-methyl-1-propanol) and one organic acid (acetic acid). The limits of detection (0.05-7.2 ppb) and relative standard deviations (0.6-11%) should be sufficient for detecting these markers of human presence in field conditions. This study shows that GC-IMS can be used as a portable field detector of hidden or entrapped people.

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“…However, the high reactivity and instability of MDA might inhibit its use as a gas‐phase biomarker. Given our recent focus on the exploitation of SIFT‐MS analysis of exhaled breath and headspace of mammalian and bacterial cell cultures when MDA has often been mentioned, we now feel constrained to investigate the potentially rewarding prospect of gas‐phase MDA analysis. Thus, in the present study we describe a method for the accurate real‐time quantification of gas‐phase MDA using selected ion flow tube mass spectrometry (SIFT‐MS) supported by solid‐phase microextraction coupled with gas chromatography/mass spectrometry (SPME‐GC/MS) for compound confirmation.…”

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confidence: 99%

Direct detection and quantification of malondialdehyde vapour in humid air using selected ion flow tube mass spectrometry supported by gas chromatography/mass spectrometry

Shestivská

Antonowicz

Dryahina

et al. 2015

Rapid Comm Mass Spectrometry

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RATIONALE:It has been proposed that malondialdehyde (MDA) reflects free oxygen-radical lipid peroxidation and can be useful as a biomarker to track this process. For the analysis of MDA molecules in humid air by selected ion flow tube mass spectrometry (SIFT-MS), the rate coefficients and the ion product distributions for the reactions of the SIFT-MS reagent ions with volatile MDA in the presence of water vapour are required. METHODS:The SIFT technique has been used to determine the rate coefficients and ion product distributions for the reaction of H3O + , NO + and O2 +• with gas phase MDA. Then, in support of SIFT-MS analysis of MDA, solid phase microextraction, SPME, coupled with gas chromatography mass spectrometry, GC/MS, has been used to confirm MDA identification. RESULTS:The primary product ions have been identified for the reactions of H3O CONCLUSIONS:This detailed study has provided the kinetics data required for the SIFT-MS analysis of MDA in humid air, including exhaled breath and the headspace of liquid phase biogenic media. Consequently, the quantification of MDA in humid gaseous media can now be used to assess oxidative stress in biogenic systems.

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Exhaled breath concentrations of acetic acid vapour in gastro-esophageal reflux disease

Cited by 44 publications

References 22 publications

Breath concentration of acetic acid vapour is elevated in patients with cystic fibrosis

Breath concentration of acetic acid vapour is elevated in patients with cystic fibrosis

Monitoring of selected skin- and breath-borne volatile organic compounds emitted from the human body using gas chromatography ion mobility spectrometry (GC-IMS)

Direct detection and quantification of malondialdehyde vapour in humid air using selected ion flow tube mass spectrometry supported by gas chromatography/mass spectrometry

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