Exfoliated Black Phosphorus Promotes in Vitro Bone Regeneration and Suppresses Osteosarcoma Progression through Cancer-Related Inflammation Inhibition

Raucci, Maria Grazia; Fasolino, Ines; Caporali, Maria; Serrano‐Ruiz, Manuel; Soriente, Alessandra; Peruzzini, Maurizio; Ambrosio, Luigi

doi:10.1021/acsami.8b21592

Cited by 72 publications

(56 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, many efforts have demonstrated that novel degradable 2D nanomaterials especially nanoclay particles and black phosphorus (BP) nanosheets with excellent bioactivity could induce osteogenic differentiation of stem cells . The innate osteoinductive performance of nanoclay were attributed to both biophysical properties (size, physical shape, and surface charge) and biochemical signaling (mineral ions dissolution products) of the nanomaterials, whereas the remarkable osteoinduction capability of BP were mainly mediated by in situ phosphorus driven, calcium‐extracted mineralized extracellular matrix during the process of BP degradation . Indeed, phosphorus is an essential element constituting about 1% of total body weight in humans, primarily as a constituent of bone .…”

Section: Introductionmentioning

confidence: 99%

Multifunctional Nanoengineered Hydrogels Consisting of Black Phosphorus Nanosheets Upregulate Bone Formation

Wang

Zhao

Tang

et al. 2019

Small

View full text Add to dashboard Cite

Tissue‐engineered hydrogels have received extensive attention as their mechanical properties, chemical compositions, and biological signals can be dynamically modified for mimicking extracellular matrices (ECM). Herein, the synthesis of novel double network (DN) hydrogels with tunable mechanical properties using combinatorial screening methods is reported. Furthermore, nanoengineered (NE) hydrogels are constructed by addition of ultrathin 2D black phosphorus (BP) nanosheets to the DN hydrogels with multiple functions for mimicking the ECM microenvironment to induce tissue regeneration. Notably, it is found that the BP nanosheets exhibit intrinsic properties for induced CaP crystal particle formation and therefore improve the mineralization ability of NE hydrogels. Finally, in vitro and in vivo data demonstrate that the BP nanosheets, mineralized CaP crystal nanoparticles, and excellent mechanical properties provide a favorable ECM microenvironment to mediate greater osteogenic cell differentiation and bone regeneration. Consequently, the combination of bioactive chemical materials and excellent mechanical stimuli of NE hydrogels inspire novel engineering strategies for bone‐tissue regeneration.

show abstract

Section: Introductionmentioning

confidence: 99%

Multifunctional Nanoengineered Hydrogels Consisting of Black Phosphorus Nanosheets Upregulate Bone Formation

Wang

Zhao

Tang

et al. 2019

Small

View full text Add to dashboard Cite

show abstract

“…Recently, studies have shown that tumor-associated inflammation, which is the seventh feature of cancer, is involved in every step of tumorigenesis and cancer progression [15][16]. were significantly associated with decreased OS in locally advanced or metastatic PC [18].…”

mentioning

confidence: 99%

Systemic Inflammatory Biomarkers, Especially Fibrinogen to Albumin Ratio, Predict Prognosis in Patients with Pancreatic Cancer

et al. 2021

View full text Add to dashboard Cite

Systemic inflammatory response is a critical factor that promotes the initiation and metastasis of malignancies including pancreatic cancer (PC). This study was designed to determine and compare the prognostic value of neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), monocyte-to-lymphocyte ratio (MLR), and fibrinogen-to-albumin ratio (FAR) in resectable PC and locally advanced or metastatic PC. Materials and Methods Three hundred fifty-three patients with resectable PC and 807 patients with locally advanced or metastatic PC were recruited in this study. These patients were classified into a training set (n=758) and a validation set (n=402). Kaplan-Meier survival plots and Cox proportional hazards regression models were used to analyze prognosis. Results Overall survival (OS) was significantly better for patients with resectable PC with low preoperative PLR (p=0.048) and MLR (p=0.027). Low FAR, MLR, NLR (p<0.001) and PLR (p=0.003) were significantly associated with decreased risk of death for locally advanced or metastatic PC patients. FAR (hazard ratio [HR], 1.522; 95% confidential interval [CI], 1.261 to 1.837; p<0.001) and MLR (HR, 1.248; 95% CI, 1.017 to 1.532; p=0.034) were independent prognostic factors for locally advanced or metastatic PC. Conclusion The prognostic roles of FAR, MLR, NLR, and PLR in resectable PC and locally advanced or metastatic PC were different. FAR showed the most prognostic power in locally advanced or metastatic PC. Low FAR was positively correlated with OS in locally advanced or metastatic PC, which could be used to predict the prognosis.

show abstract

“…The unique geometric and electronic structure of nano‐BP endows them many appealing surface properties and photoelectric properties that show great promising in biomedical and biosensing applications . Within every layer of BP with honeycomb structure, each phosphorus atom forms three covalent bonds with the adjacent phosphorus atoms, leaving one pair of electrons exposed outside.…”

Section: Nano‐bp Functionalizationmentioning

confidence: 99%

Recent Advances on Black Phosphorus for Biomedicine and Biosensing

Xia

Guo

2019

Adv Funct Materials

192

115

View full text Add to dashboard Cite

Black phosphorus nanostructures (nano-BPs) include BP nanosheets, BP quantum dots, and BP nanoparticles. Since first being discovered in 2014, nano-BP has become one of the most popular nanomaterials. Nano-BP has many unique properties, such as excellent surface activity, tunable bandgap, high carrier mobility, moderate on/off ratio, excellent biocompatibility, good biodegradation, etc., all of which make nano-BP particularly attractive in biomedicine and biosensing. This review article comprehensively summarizes recent advances in synthesis, functionalization, biomedicine, and biosensing applications of nano-BP. Different methods are first introduced, such as mechanical cleavage, liquid-phase ultrasonic exfoliation, electrochemical exfoliation, solvothermal treatment, and acoustic-microfluidic stripping, for making the nano-BP. Then two strategies are emphasized to enhance ambient stability of nano-BP, namely physical encapsulation and chemical modification. Next, how to develop nano-BP as advanced imaging agents, nanocarriers, and nanomedicine for bioimaging (fluorescence imaging, thermal imaging, and photoacoustic imaging) and disease treatment (phototherapy and photo/chemical/immune synergistic therapy) is demonstrated. The biosensing applications on nano-BP is introduced, including electrochemical biosensor, fluorescence biosensor, chemiluminescence biosensor, electrogenerated chemiluminescence biosensor, and colorimetric biosensor. Finally, the current challenges and future perspectives on nano-BP in bioapplications are discussed. Nano-BP SynthesisLayered nanomaterials are usually exfoliated from bulk crystals. [35] Compared with bulk materials, monolayer or fewlayer nanomaterials possess the enhanced surface area, excellent physiochemical activity and optoelectronic property. The exfoliation from bulk crystal to monolayer or few-layer nanosheets has been widely applied for preparing almost all the 2D nanomaterials, graphene, MoS 2 , MXene nanosheets, etc. [36] Bulk BP, similar to graphite, is the stacking of layered BP nanosheets via the weak van der Waals force. The destruction of the external driving forces to the weak van der Waals interaction between the stack layers greatly contributes to the exfoliation of nano-BP from the bulk counterparts. Mechanical exfoliation and liquid phase ultrasonic exfoliation are the two most popular approaches, and some other methods were also involved, electrochemical exfoliation and solvothermal treatment, for instance. Table 1 summarizes the advantages and limitations of different exfoliation methods. Mechanical ExfoliationMechanical exfoliation, also called scotch-tape delamination, mainly utilizes the larger binding energy between the substrate and layered materials than van der Waals forces in Figure 2. a) Schematic illustration of meta-assisted mechanical exfoliation for FLBP. b) The left is FLBPs exfoliated using the normal scotch-tape microcleavage, and the right is FLBP exfoliated using Au-assisted method, and c) the total area of FLBP on 10 different samples. ...

show abstract

Exfoliated Black Phosphorus Promotes in Vitro Bone Regeneration and Suppresses Osteosarcoma Progression through Cancer-Related Inflammation Inhibition

Cited by 72 publications

References 36 publications

Multifunctional Nanoengineered Hydrogels Consisting of Black Phosphorus Nanosheets Upregulate Bone Formation

Multifunctional Nanoengineered Hydrogels Consisting of Black Phosphorus Nanosheets Upregulate Bone Formation

Systemic Inflammatory Biomarkers, Especially Fibrinogen to Albumin Ratio, Predict Prognosis in Patients with Pancreatic Cancer

Recent Advances on Black Phosphorus for Biomedicine and Biosensing

Contact Info

Product

Resources

About