Exercise training protects against an acute inflammatory insult in mouse epididymal adipose tissue

Castellani, L; Root-McCaig, Jared; Frendo‐Cumbo, Scott; Beaudoin, Marie-Soleil; Wright, David C.

doi:10.1152/japplphysiol.00074.2014

Cited by 37 publications

(56 citation statements)

References 33 publications

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“…In support of our data, others have shown that 6 weeks of VWR 38 and exercise training 30 protect against acute stressors. For example, our group has shown that exercise training offers protection against adipose tissue inflammation induced by the b-3 adrenergic agonist CL 316, 243.…”

Section: Discussionsupporting

confidence: 92%

“…For example, our group has shown that exercise training offers protection against adipose tissue inflammation induced by the b-3 adrenergic agonist CL 316, 243. 30 In this study, protective effects were primarily observed in eWAT for indices of IL-6 signaling as marked by the mRNA expression of IL-6 and SOCS3, along with the mRNA expression of TNF-a, yet a similar effect did not occur in iWAT. Others 38 have demonstrated that 6 weeks of VWR alters the response to acute tail shock stress in rats.…”

Section: Discussionmentioning

confidence: 57%

“…1A-E). As previous data from our group and that from others have shown that exercise training (swimming, 11 treadmill running 30 ) and VWR 9 increase indices of mitochondrial biogenesis, we measured the protein content of PGC-1a, a transcriptional co-activator and master regulator of mitochondrial proteins, 31 as well as other mitochondrial proteins in iWAT and eWAT. In iWAT, there was main effect of VWR for increasing the protein content of numerous markers of mitochondrial biogenesis including PGC-1a (p < 0.001), CytC (p < 0.01), COXIV (p < 0.001), PDH-E1a (p < 0.001), CORE1 (p < 0.001), and CS (p < 0.001) ( Fig.…”

Section: Vwr Attenuates Body Mass Gain Improves Glucose Tolerance Amentioning

confidence: 99%

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Habitual physical activity protects against lipopolysaccharide-induced inflammation in mouse adipose tissue

et al. 2016

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Sepsis is a systemic inflammatory response to infection, with no preventative strategies. In this study, we identify a role for habitual physical activity in the prevention of adipose tissue inflammation induced by a model of sepsis, lipopolysaccharide (LPS). Male C57BL/6J mice (8 weeks old) were housed with access to voluntary wheel running (VWR) or sedentary (SED) for 10 weeks. Mice were then injected with LPS (2 mg/kg) or saline (SAL), and tissues were removed 6 hours postinjection. VWR attenuated body, epididymal adipose tissue (eWAT), and subcutaneous inguinal adipose tissue (iWAT) mass gain, improved glucose tolerance, increased markers of mitochondrial biogenesis in iWAT and eWAT, and increased UCP-1 protein content in iWAT. In iWAT, VWR attenuated the LPS induced increase in mRNA expression of TNF-a, MCP-1, and follistatin, along with phosphorylation of STAT3. In addition, VWR had a main effect for reducing iWAT mRNA expression of IL-1b, IL-6, and SOCS3. In eWAT, VWR had a main effect for reducing mRNA expression of IL-1b, MCP-1, IL-6, and follistatin. Further, VWR increased SOCS3 mRNA expression and phosphorylation of STAT3 in SAL mice, thus the relative change in response to LPS for these markers was attenuated. The protective effect of prior physical activity occurred in conjunction with increases in the protein content of a component of the LPS binding complex, MyD88. Overall, the results from this study demonstrate that habitual physical activity can attenuate the LPS induced inflammatory response in adipose tissue and this occurs to a greater extent in iWAT compare with eWAT.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 57%

Section: Vwr Attenuates Body Mass Gain Improves Glucose Tolerance Amentioning

confidence: 99%

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Habitual physical activity protects against lipopolysaccharide-induced inflammation in mouse adipose tissue

et al. 2016

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“…This compound has been used in other studies to stimulate lipolysis in white adipose tissue. 33,34 30 minutes post-saline or CL injection, inguinal adipose tissue (iWAT) was removed as a representative subcutaneous depot; retroperitoneal (r.p.) adipose tissue was harvested from behind the kidneys as a representative visceral depot.…”

Section: Terminal Surgeries and In Vivo Lipolysis Assessmentmentioning

confidence: 99%

Estradiol does not directly regulate adipose lipolysis

et al. 2017

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The mechanisms by which estradiol modulates adipose lipolysis are poorly understood. We sought to measure basal and b 3 -stimulated indices of lipoysis (FFAs, glycerol) in vivo in E2 deficient or supplemented rats, and ex vivo with direct acute E2 exposure. For 2 weeks, ovariectomized (OVX) and OVX rats treated with a daily oral dose of E2 (OVX E2) were pairfed to SHAM controls (n D 12 per group). Adipocyte size was modestly (»40%) increased in OVX rats, but did not reach significance (p D 0.2). After 2 weeks, half of the animals in each group received an in vivo injection of saline or 1 mg/kg of the b3 agonist CL 316, 243. Serum FFA concentrations, but not glycerol, were lower in OVX and OVX E2 rats compared with SHAM controls (p D 0.02). A significant CL response was present in all groups (p<0.001) and HSL activation was unaffected by OVX or OVX E2 in retroperitoneal (r.p.) or inguinal (iWAT) adipose depots in vivo. Ex vivo, CL increased FFA and glycerol accumulation in the media as well as HSL phosphorylation by several fold in r.p. and iWAT explants, but responses from OVX and OVX E2 rats were comparable to SHAMs. To assess whether E2 can directly affect lipolysis, r.p. and iWAT tissue was treated with E2, CL or E2 C CL for 2, 4 or 8 hours using adipose tissue organ culture. CL stimulated FFA release (p<0.001), but was unaffected by E2. Overall, our results indicate that E2 does not directly regulate adipose tissue lipolysis.

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“…In regards to lipolysis and TRPV4, Farias et al [5] reported that continuous swimming training robustly induced ATGL with no effect on CGI-58 protein expression in the epididymal fat of diet-induced obese (DIO) mice. Castellani et al [3] reported that treadmill training reduced TRPV4 protein content in epididymal fat of chow fed mice and the reduction of TRPV4 could mediate the protective effects of prior exercise training against CL 316,243 induced inflammatory status in AT. However, how exercise training will affect TRPV4 and its associated protein expression in adipose tissue of DIO mice remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Effects of treadmill running and rutin on lipolytic signaling pathways and TRPV4 protein expression in the adipose tissue of diet-induced obese mice

et al. 2015

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To explore the effects of rutin and exercise on high-fat diet (HFD)-induced disrupted lipolytic signaling, adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) signaling, transient receptor potential cation channel subfamily V member 4 (TRPV4) and its associated protein expression, and whether depot-specific effects existed. C57BL/6J mice were randomized into five groups: chow group, HFD, HFD plus rutin intervention group (HR), HFD combined with treadmill running group (HE), and HFD combined with treadmill running and rutin intervention group (HRE). At the end of the 16-week intervention, lipolytic markers, AMPK signaling pathways, TRPV4, and peroxisome proliferator-activated receptor gamma coactivator 1α + β (PGC-1α + β) from adipose tissue were measured by western blotting. In epididymal adipose tissue, HFD resulted in significant reduction in the phosphorylation of hormone sensitive lipase at serine660 (p-HSL660), perilipin A, phosphoenolpyruvate carboxykinase (PEPCK), p-AMPK, and p-acetyl-CoA carboxylase (ACC) protein expression. Exercise intervention and exercise plus rutin completely restored p-HSL660, perilipin A, PEPCK, p-AMPK, and p-ACC protein expression to normal level. HFD and HR groups have reduced expression of PGC-1α + β, exercise, and exercise plus rutin completely restored PGC-1α + β expression to normal level. In subcutaneous adipose tissue, HFD elevated TRPV4, exercise, and exercise plus rutin completely reduced TRPV4 to normal level. HR, HE, and HRE group have increased PGC-1α + β. In conclusion, depot-specific effects existed in regards to how rutin and exercise affect lipolytic signaling and p-AMPK, as well as TRPV4 and PGC-1α + β expression.

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Exercise training protects against an acute inflammatory insult in mouse epididymal adipose tissue

Cited by 37 publications

References 33 publications

Habitual physical activity protects against lipopolysaccharide-induced inflammation in mouse adipose tissue

Habitual physical activity protects against lipopolysaccharide-induced inflammation in mouse adipose tissue

Estradiol does not directly regulate adipose lipolysis

Effects of treadmill running and rutin on lipolytic signaling pathways and TRPV4 protein expression in the adipose tissue of diet-induced obese mice

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