Exercise training partially rescues impaired mucosal associated invariant t-cell mobilization in breast cancer survivors compared to healthy older women

Hanson, Erik D.; Bates, Lauren; Harrell, Elizabeth P.; Bartlett, David B.; Lee, Jordan T.; Wagoner, Chad W.; Alzer, Mohamdod; Amatuli, Dean J.; Jensen, Brian C.; Deal, Allison M.; Muss, Hyman B.; Nyrop, Kirsten A.; Battaglini, Cláudio L.

doi:10.1016/j.exger.2021.111454

Cited by 8 publications

(26 citation statements)

References 51 publications

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“…For Outcome 1, descriptive statistics (mean ± SD) are shown for baseline characteristics and compared across groups (Lean vs. OW) via t-test. For Outcomes 2 and 3, mobilization and egress were analyzed separately using a linear mixed model [19]. The fixed factors were group and time with subjects as a random effect.…”

Section: Discussionmentioning

confidence: 99%

“…Currently, the literature examining unconventional T-cell response to exercise [7], and especially MAIT-cell response, is quite limited. To date, only acute aerobic exercise in healthy young men [16,17] and acute and chronic exercise in breast cancer survivors [19] have been published. The limitations and strengths of the study are presented to contextualize the findings of the present study.…”

Section: Discussionmentioning

confidence: 99%

“…Blood was collected into chilled ethylenediaminetetraacetic acid (EDTA) tubes following manufacturer's instructions (Invitrogen, Carlsbad, CA, USA) and analyzed using ActDiff 5 hematology analyzer (Beckman Coulter, Brea, CA, USA). PBMCs were cryopreserved utilizing methodology previously described [19]. At the end of the study, PBMCs were thawed in a 37 • C warm water bath.…”

Section: Blood Analysismentioning

confidence: 99%

“…PBMCs were allowed to rest for 2 h at 37 • C and 5% CO 2 (confirmed by pilot work). Cell viability was again assessed and then cells were stimulated with 2 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 ng/mL Ionomycin for 4 h at 37 • C and 5% CO 2 , as used previously [19].…”

Section: Blood Analysismentioning

confidence: 99%

“…MAIT-cells have displayed an intensity dependent numerical mobilization similar to conventional CD8+ T-cells [18]. Exercise training has been shown to partially rescue acute exercise-induced MAIT-cell mobilization in older breast cancer survivors [19]. Although the population of breast cancer survivors was not overweight, breast cancer and its treatment results in similar proinflammatory and reduced immune function as obesity.…”

Section: Introductionmentioning

confidence: 99%

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Mucosal-Associated Invariant T Cell Response to Acute Exercise and Exercise Training in Older Obese Women

Bates

Hanson

Levitt

et al. 2021

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(1) Background: Obesity is a major global public health concern as it is associated with many of the leading causes of preventable deaths. Exercise reduces obesity-induced inflammation; however, it is unknown how exercise training may impact mucosal associated invariant T (MAIT) cells in overweight/obese (OW) post-menopausal women. Therefore, the purpose of this study was to investigate (i) circulating MAIT-cells at rest in OW vs. Lean women, (ii) the response of MAIT-cells to a single bout of combined aerobic and resistance exercise, and (iii) the effects of 12 weeks of exercise training (EX) or educational program (ED) on the MAIT-cell response in OW. (2) Methods: OW completed an acute exercise session or sitting control, underwent 12 weeks of exercise training or received educational materials, and then repeated the exercise session/sitting control. Lean post-menopausal women provided a baseline comparison. (3) Results: OW had lower circulating MAIT-cells at rest than Lean prior to exercise training; however, after training EX displayed improved MAIT-cell frequency. Additionally, prior to training EX did not exhibit MAIT-cell mobilization/egress, however, both improved after training. (4) Conclusions: Reduced MAIT-cell frequency and ability to mobilize/egress were potentially partially rescued in EX after 12 weeks of exercise training; however, further research is needed to elucidate age or obesity-induced attenuations in MAIT-cells.

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Section: Discussionmentioning

confidence: 99%