Exercise test and glucose homeostasis in rats treated with alloxan during the neonatal period or fed a high calorie diet

Abstract

Background: Animal models appear well‐suited for studies into the role of exercise in the prevention of non‐insulin‐dependent diabetes mellitus (NIDDM). The aim of the present study was to analyze glucose homeostasis and blood lactate during an exercise swimming test in rats treated with alloxan during the neonatal period and/or fed a high calorie diet from weaning onwards. Methods: Rats were injected with alloxan (200 mg/kg, i.p.) or vehicle (citrate buffer) at 6 days of age. After weaning, rats were divided into four groups and fed either a balanced diet or a high‐caloric diet as follows: C, control group (vehicle + normal diet); A, alloxan‐treated rats fed the normal diet; H, vehicle‐treated rats fed the high‐caloric diet; and HA, alloxan‐treated rats fed the high‐caloric diet. Results: Fasting serum glucose levels were higher in groups A and AH compared with the control group. The Homeostatic Model Assessment index varied in the groups as follows: H>A>HA = C. There were no differences in free fatty acids or blood lactate concentrations during the swim test. Conclusions: Alloxan‐treated rats fed a normal or high‐caloric diet have the potential to be used in studies analyzing the role physical exercise plays in the prevention of NIDDM.

Continuous and Intermittent Exercise Training and Glucose Metabolism in Neonatal Alloxan Administered Rats

“…It is noteworthy that, in the present study, the animals had reduced body weight due to the increased energy expenditure required during exercise and not due to At 28 days, we observed glucose intolerance and insulin resistance in alloxan animals, after a greater area under the curve for serum glucose and insulin was observed during the OGTT, and we observed a lower Kitt during the ITT for alloxan animals. Similar results were reported in Oliveira et al [15] and Contarteze et al [16]. Together, these data indicate the effectiveness of neonatal administration of alloxan in the alteration of glucose homeostasis in rats.…”

“…Moreover, both the continuous and intermittent trainings were effective in reducing the area under the curve for insulinemia in alloxan and control groups. Unlike Contarteze et al [16], who found differences between the control and alloxan groups in insulin sensitivity by the HOMA index, in this present study, no difference between groups in insulin sensitivity was observed when we analyzed the Kitt.…”

“…On the other hand, Contarteze et al [16] showed that these animals presented higher blood glucose concentrations after an oral glucose load compared to controls. Ribeiro et al [17], using rats that received alloxan at the 6th day of life, also showed higher blood glucose values during a glucose tolerance test in the alloxan treated animals compared to the control group, at both 28 and 60 days of age.…”

Modulation of Epileptic Activity in Rats: Focus on Sleep, Physical Exercise and Nitric Oxide–mediated Neurotransmission in a Model of Homocysteine Thiolactone–induced Seizures

“…Hrncic et al (60) investigated the effects of regular physical activity on the epileptic activity induced by homocysteine thiolactone using an experimental paradigm of aerobic physical activity on a treadmill. Namely, rats were made to run on treadmill apparatus for 30 minutes once a day for 30 consecutive days while the belt speed was set to 20 m/min with a 0° incline.…”

Ameliorating effect of hawthorn (<i>Crataegus oxyacantha</i>) and physical exercise on acute penicillin induced seizures in gerbils

“…However, an exercise-induced increase in epileptiform EEG activity has also been reported (Horyd et al, 1981;Nakken et al, 1997). Treadmill running training is considered as the most frequently used aerobic exercise type in the experimental studies (Contarteze et al, 2009). …”