Exercise running and tetracycline as means to enhance skeletal muscle stem cell performance after external fixation

Shefer, Gabi; Carmeli, Eli; Rauner, Gat; Yablonka–Reuveni, Zipora; Benayahu, Dafna

doi:10.1002/jcp.21306

Cited by 22 publications

(22 citation statements)

References 81 publications

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“…Additional studies suggested mesenchymal plasticity of satellite cells based on detecting nonmyogenic cells, including adipocytes, in cultures emanating from single myofibers [69], [71]. Similar to results presented here, nonmyogenic cells were also detected in clonal assays of myofiber-associated cells [11], [71], [72], [73]. It is possible, however, that some or most nonmyogenic cells that develop in myofiber cultures are derived from cells that are in close association with myofibers but not necessarily from Pax7 + satellite cells [70], [74], [75], [76].…”

Section: Discussionsupporting

confidence: 85%

“…As shown across different muscle groups and species, Pax7 protein is expressed by satellite cells, but not by myofiber nuclei or non-myogenic cell types present in the adult muscle tissue [8], [9], [10], [11]. Proliferating progeny of satellite cells, myoblasts, maintain Pax7 protein expression and upregulate the expression of MyoD, a muscle specific transcription factor.…”

Section: Introductionmentioning

confidence: 96%

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Reduced Satellite Cell Numbers and Myogenic Capacity in Aging Can Be Alleviated by Endurance Exercise

et al. 2010

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BackgroundMuscle regeneration depends on satellite cells, myogenic stem cells that reside on the myofiber surface. Reduced numbers and/or decreased myogenic aptitude of these cells may impede proper maintenance and contribute to the age-associated decline in muscle mass and repair capacity. Endurance exercise was shown to improve muscle performance; however, the direct impact on satellite cells in aging was not yet thoroughly determined. Here, we focused on characterizing the effect of moderate-intensity endurance exercise on satellite cell, as possible means to attenuate adverse effects of aging. Young and old rats of both genders underwent 13 weeks of treadmill-running or remained sedentary.MethodologyGastrocnemius muscles were assessed for the effect of age, gender and exercise on satellite-cell numbers and myogenic capacity. Satellite cells were identified in freshly isolated myofibers based on Pax7 immunostaining (i.e., ex-vivo). The capacity of individual myofiber-associated cells to produce myogenic progeny was determined in clonal assays (in-vitro). We show an age-associated decrease in satellite-cell numbers and in the percent of myogenic clones in old sedentary rats. Upon exercise, there was an increase in myofibers that contain higher numbers of satellite cells in both young and old rats, and an increase in the percent of myogenic clones derived from old rats. Changes at the satellite cell level in old rats were accompanied with positive effects on the lean-to-fat Gast muscle composition and on spontaneous locomotion levels. The significance of these data is that they suggest that the endurance exercise-mediated boost in both satellite numbers and myogenic properties may improve myofiber maintenance in aging.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 96%

Reduced Satellite Cell Numbers and Myogenic Capacity in Aging Can Be Alleviated by Endurance Exercise

et al. 2010

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“…Certainly, hind limb immobilization studies have demonstrated that periods of inactivity both reduce satellite cell number and detrimentally affect proliferation and differentiation potential, 38 the effects of which are reversible. 39 Many studies have reported increased adiposity of skeletal muscle with age 21,22,40 and although this was not directly attributable to satellite cell transdifferentiation, studies have shown that satellite cells isolated from both mouse 18 and human 41 myofibres can undergo adipogenesis. Our results that SM derived satellite cells give rise to adipocytes more readily than diaphragm suggest that intermuscular differences may influence such differentiation during the aging process.…”

Section: Discussionmentioning

confidence: 98%

Muscle origin of porcine satellite cells affects in vitro differentiation potential

Redshaw¹,

McOrist²,

Loughna³

2010

Cell Biochemistry & Function

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Post-natal muscle regeneration relies on the activation of tissue stem cells known as satellite cells, to repair damage following exercise trauma and disease. Satellite cells from individual muscles are known to be heterogeneous with regard to proliferation, fusion and transplantation abilities, although the muscle origin has rarely been considered pertinent to their differentiation capabilities. In this study we compared the potential of two functionally distinct skeletal muscle satellite cell populations from porcine diaphragm and hind-limb semi-membranosus muscles. These two muscles were chosen primarily for differences in metabolic and contractile properties: the diaphragm is more continuously active and has a greater oxidative capacity. Cells were induced to differentiate towards myogenic and adipogenic lineages, and here we have shown that cells from diaphragm exhibit a significantly greater degree of myogenesis compared with those from semi-membranosus, while the converse was true for adipogenesis. Unexpectedly, both conditions generated small numbers of cells with neuronal characteristics for both muscle types, although more so in cells derived from the diaphragm. With increased interest in muscle adiposity with age and disease, these findings suggest that muscle origin of satellite cells does affect lineage fate, however whether differences in developmental origin or metabolic activity of the parent tissue govern this, remains to be determined.

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“…Standard rat pellets and water were provided ad lib. Three rats from each age group (a total of 9 rats) ran on a treadmill for two weeks, as previously described [21]. The running speed of old animals was 0.5 km/hr and of young and adult animals was 0.9 Km/hr.…”

Section: Methodsmentioning

confidence: 99%

“…Isolation and Culture of EDL Myofibers Muscle fibers with associated satellite cells were isolated from the gastrocnemius muscle as previously described [21]. Myofibers were incubated at 37.5°C, 5% CO 2 in a humidified tissue culture incubator.…”

Section: Methodsmentioning

confidence: 99%

SVEP1 is a Novel Marker of Activated Pre-determined Skeletal Muscle Satellite Cells

Shefer

Benayahu

2010

Stem Cell Rev and Rep

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In this study we explored the expression pattern of SVEP1, a novel cell adhesion molecule (CAM), in bona fide satellite cells and their immediate progeny. We show that SVEP1 is expressed in activated satellite cells prior to their determination to the myogenic lineage. SVEP1 was also expressed during early phases of myogenic differentiation through the initial stage of myoblast fusion and myotube formation. The expression of SVEP1 was shown by immunostaining two cell culture systems: freshly isolated myofibers and primary myoblasts. Pax7 was used to pinpoint satellite cells situated in their niche on myofibers, and activated satellite cells were determined based on BrdU incorporation (Pax7(+)/BrdU(+)cells). MyoD marked satellite cells fated to undergo myogenesis as well as proliferating and differentiating myoblasts. Differentiating myoblasts and myotubes were identified based on their sarcomeric myosin expression. We showed that SVEP1 was specifically expressed in pre-determined activated satellite cells (Pax7(+)/ BrdU(+) /MyoD(-)) accounting for about 24% of total satellite cells. On the other hand, SVEP1 expression was absent in quiescent satellite cells (Pax7(+)/BrdU(-)/MyoD(-)). Moreover, based on MyoD/sarcomeric myosin co-expression SVEP1 was shown to be expressed throughout the early phases of myogenesis up until myoblast fusion and myotube formation. A decline in SVEP1 expression occurred upon myotube maturation. We suggest SVEP1 as a potential biomarker for pre-fated satellite cells. The impact of this finding is that it may allow scrutinizing signals that affect differentiation commitment. Thus, holds a therapeutic potential for maladies that involve deregulated stem cell fate-decision.

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Exercise running and tetracycline as means to enhance skeletal muscle stem cell performance after external fixation

Cited by 22 publications

References 81 publications

Reduced Satellite Cell Numbers and Myogenic Capacity in Aging Can Be Alleviated by Endurance Exercise

Reduced Satellite Cell Numbers and Myogenic Capacity in Aging Can Be Alleviated by Endurance Exercise

Muscle origin of porcine satellite cells affects in vitro differentiation potential

SVEP1 is a Novel Marker of Activated Pre-determined Skeletal Muscle Satellite Cells

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