Rationale Asthma is one of the most common chronic diseases in childhood, occurring in up to 10% of all children. Exercise induced bronchoconstriction (EIB) is indicative of uncontrolled asthma and can be assessed by means of an exercise challenge test (ECT). These tests however draw heavily on healthcare resources and require demanding repetitive forced breathing manoeuvres of children. In this study the electric activity of the respiratory muscles was measured as a tool to assess EIB. Methods Children suspected of exercise induced respiratory symptoms performed an ECT wearing a portable EMG amplifier (Dipha-16, Demcon Macawi respiratory systems, Enschede, the Netherlands). EIB was defined as a fall in FEV1 of greater than 13%. Electrodes were placed bilaterally at the diaphragm and accessory breathing (intercostal-, sternocleidomastoid-and trapezoid) muscles. A single reference electrode was placed at the sternum. Children were asked to sit still for 30 seconds after each spirometry measurement to obtain EMG measurements. Data was pre-processed and analyzed in Matlab.