Exercise Increases the Frequency of Circulating Hematopoietic Progenitor Cells, But Reduces Hematopoietic Colony-Forming Capacity

Kroepfl, Julia; Pekovits, Karin; Stelzer, Ingeborg; Fuchs, Robert; Zelzer, Sieglinde; Hofmann, Peter; Sedlmayr, Peter; Dohr, Gottfried; Wallner-Liebmann, Sandra J.; Domej, Wolfgang; Mueller, Wolfram

doi:10.1089/scd.2012.0017

Cited by 31 publications

(48 citation statements)

References 47 publications

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“…In light of the well-documented association of obesity with breast cancer, we compared in the current study the abundance of circulating ASCs in obese and non-obese breast cancer survivors. Although there is published work on the effect of exercise on circulating endothelial cells (72) and hematopoietic progenitor cells (73), similar studies on circulating ASCs have not been reported. Therefore, we sought to assess the impact of exercise on circulating ASCs among the obese breast cancer survivors.…”

Section: Introductionmentioning

confidence: 99%

Association of obesity and circulating adipose stromal cells among breast cancer survivors

et al. 2014

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A positive association of obesity with breast cancer incidence and mortality is well established. Recent reports indicate that adipose stromal cells (ASCs) play an important role in breast cancer development and progression by producing estrogens and tumor-promoting cytokines. Furthermore, circulating ASCs have been uniquely detected in obese individuals, which is likely due to increased tissue remodeling and cell mobilization. The number of circulating ASCs is even more prominent in obese patients with colon and prostate cancers, both of which are exacerbated by obesity. To determine whether a similar association exists for breast cancer, we collected blood samples from a cohort of breast cancer survivors and enumerated circulating ASCs by flow cytometry on the basis of the previously established ASC-associated immunophenotype (CD34+/CD31−/CD45−). We found significantly higher levels of circulating ASCs (p < 0.001) in breast cancer survivors with body mass index (BMI) ≥ 30 kg/m2 than their non-obese counterparts (BMI < 30). We also compared circulating ASCs before and after exercise of only the obese subjects enrolled in a 6-month individualized exercise program, but found no statistically significant difference, likely due to limited number of subjects in the study. Our findings suggest that circulating ASCs can serve as a potential biomarker for future studies of the impacts of obesity and physical activity on breast cancer recurrence and survival.

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Section: Introductionmentioning

confidence: 99%

Association of obesity and circulating adipose stromal cells among breast cancer survivors

et al. 2014

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“…One possible explanation is that IHH and ME may be more aggressive stimuli in older than for younger subjects. Some studies have reported a time-dependent increase in CPC count after a cycle incremental exercise test under normoxic or hypoxic conditions, increasing 10 min after exercise [21], during 240 min of strenuous exercise [22] and after a marathon race or 1500m field test [19]. …”

Section: Discussionmentioning

confidence: 99%

Combined intermittent hypobaric hypoxia and muscle electro-stimulation: a method to increase circulating progenitor cell concentration?

Corral

Javierre

Blasi

et al. 2014

Journal of Translational Medicine

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BackgroundOur goal was to test whether short-term intermittent hypobaric hypoxia (IHH) at a level well tolerated by healthy humans could, in combination with muscle electro-stimulation (ME), mobilize circulating progenitor cells (CPC) and increase their concentration in peripheral circulation.MethodsNine healthy male subjects were subjected, as the active group (HME), to a protocol involving IHH plus ME. IHH exposure consisted of four, three-hour sessions at a barometric pressure of 540 hPa (equivalent to an altitude of 5000 m). These sessions took place on four consecutive days. ME was applied in two separate 20-minute periods during each IHH session. Blood samples were obtained from an antecubital vein on three consecutive days immediately before the experiment, and then 24 h, 48 h, 4 days, 7 days and 14 days after the last day of hypoxic exposure. Four months later a control study was carried out involving seven of the original subjects (CG), who underwent the same protocol of blood samples but without receiving any special stimulus.ResultsIn comparison with the CG the HME group showed only a non-significant increase in the number of CPC CD34+ cells on the fourth day after the combined IHH and ME treatment.ConclusionCPC levels oscillated across the study period and provide no firm evidence to support an increased CPC count after IHH plus ME, although it is not possible to know if this slight increase observed is physiologically relevant. Further studies are required to understand CPC dynamics and the physiology and physiopathology of the hypoxic stimulus.

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“…Although previous studies have investigated the influence of physical exercise on total CPC numbers by flow cytometry [1], [2], [8] and colony-forming unit (CFU) assays [2], the proliferative capacity/functionality of CPCs in humans has only been addressed in our study [6]. The important question of which strenuous exercise-induced mechanisms affect CPC functionality, however, remains open and needs further elucidation.…”

Section: Introductionmentioning

confidence: 97%

“…Exercise has a complex influence on the body, making the exact mechanisms responsible for CPC mobilization and the influence on CPC functionality/proliferative capacity difficult to identify [5]. Previous studies have associated exercise-induced oxidative stress [6], cortisol (Co) [7], interleukin 6 (IL-6), growth hormones such as vascular endothelial growth factor (VEGF) [8], and granulocyte-colony stimulating factor (G-CSF) [9] with post-exercise increased peripheral CPC numbers. A possible influence of exercise-related peripheral blood (pb) free/bound norepinephrine (NE), free/bound epinephrine (EPI) and lactate (La) remains to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

“…Circulating hematopoietic stem and progenitor cell functionality means that CPCs are able to keep their proliferative capacity at a high level. This is analyzed by a secondary colony-forming unit granulocyte monocyte (CFU-GM) assay [6], [16]–[18]. Using this functional assay, we have previously shown the higher proliferative capacity of stem and progenitor cells in the bone marrow of voluntary life-long exercising rats [16].…”

Section: Introductionmentioning

confidence: 99%

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Exercise-Induced Norepinephrine Decreases Circulating Hematopoietic Stem and Progenitor Cell Colony-Forming Capacity

et al. 2014

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A recent study showed that ergometry increased circulating hematopoietic stem and progenitor cell (CPC) numbers, but reduced hematopoietic colony forming capacity/functionality under normoxia and normobaric hypoxia. Herein we investigated whether an exercise-induced elevated plasma free/bound norepinephrine (NE) concentration could be responsible for directly influencing CPC functionality. Venous blood was taken from ten healthy male subjects (25.3+/−4.4 yrs) before and 4 times after ergometry under normoxia and normobaric hypoxia (FiO2<0.15). The circulating hematopoietic stem and progenitor cell numbers were correlated with free/bound NE, free/bound epinephrine (EPI), cortisol (Co) and interleukin-6 (IL-6). Additionally, the influence of exercise-induced NE and blood lactate (La) on CPC functionality was analyzed in a randomly selected group of subjects (n = 6) in vitro under normoxia by secondary colony-forming unit granulocyte macrophage assays. Concentrations of free NE, EPI, Co and IL-6 were significantly increased post-exercise under normoxia/hypoxia. Ergometry-induced free NE concentrations found in vivo showed a significant impairment of CPC functionality in vitro under normoxia. Thus, ergometry-induced free NE was thought to trigger CPC mobilization 10 minutes post-exercise, but as previously shown impairs CPC proliferative capacity/functionality at the same time. The obtained results suggest that an ergometry-induced free NE concentration has a direct negative effect on CPC functionality. Cortisol may further influence CPC dynamics and functionality.

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Exercise Increases the Frequency of Circulating Hematopoietic Progenitor Cells, But Reduces Hematopoietic Colony-Forming Capacity

Cited by 31 publications

References 47 publications

Association of obesity and circulating adipose stromal cells among breast cancer survivors

Association of obesity and circulating adipose stromal cells among breast cancer survivors

Combined intermittent hypobaric hypoxia and muscle electro-stimulation: a method to increase circulating progenitor cell concentration?

Exercise-Induced Norepinephrine Decreases Circulating Hematopoietic Stem and Progenitor Cell Colony-Forming Capacity

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