Exercise improves hemodynamic profiles and increases red blood cell concentrations of purine nucleotides in a rodent model

Abstract

Exercise increased RBC ATP concentrations in a rodent model, which was correlated with the decrease in BP and HR postexercise.

Anti-Ischemia Drugs have no Effect on the In Vivo Metabolism of ATP by RBC in Normotensive Restrained Rats#

“…This suggests that RBC concentrations of ATP and AMP may be linked to the decline of DBP. A similar relationship between DBP and RBC concentrations of ATP was also found in rats after exercise [31]. The mechanism for the decrease of DBP is not known, although it could be attributed to reduced energy utilization secondary to lack of physical activity as the rats were kept in the restrainer for the entire experiment.…”

“…Previous studies from our laboratories have shown that DTZ increased RBC concentrations of ATP in an exercise rat model and zebrafish model [36,37], and that RBC concentrations of ATP may be sensitive biomarkers for cardiovascular protec-tion [31,35]. It is possible that anti-ischemia agents may affect metabolism of ATP in RBC either directly or indirectly which may affect their safety and efficacy.…”

“…It has been shown that patients with effort angina and essential hypertension have altered adenosine metabolism compared to normal individuals [23][24][25], and that plasma concentrations of adenosine increase in patients with congestive heart failure (CHF) [26], which could be a physiologic response to heart failure and help to reduce the severity of the disease [27]. Thus it has been postulated that adenosine and ATP may be used as sensitive biomarkers to quantify myocardial and endothelial ischemia [28][29][30], a surrogate for post-exercise hypotension [31] and for monitoring therapeutic effects of anti-ischemia drugs [32][33][34][35].…”

A Pilot Study to Assess Adenosine 5’-triphosphate Metabolism in Red Blood Cells as a Drug Target for Potential Cardiovascular Protection

“…After 2 brief sessions of 3 – 5 minutes (min) each of training to acclimatize the rat with the treadmill on the day before the study, each rat was exercised on the treadmill for 15 min at 10 m/min and 10% grade 
(n = 7) (LowEx), or 15 min at 14 m/min and 22% grade 
(n = 8) (VigEx). Another group of rats without exercise was used as control (NoEx, n = 10) as described previously [35, 36]. Two hours after the exercise, each rat received a single dose of isoproterenol (30 mg/kg) by subcutaneous (sc) injection.…”

“…Previous studies have shown that exercise preconditioning offered cardioprotective effect against injury induced by isoproterenol in rats [32, 33], and cardiotoxicity induced by doxorubicin [34]. We have recently shown that exercise improved cardio-
vascular hemodynamic profiles and increased red blood cell (RBC) concentrations of ATP in both normotensive Sprague Dawley rat (SDR) and spontaneously hypertensive rats (SHR) although the post exercise effects were more profound in the SHR [35, 36]. The increase of ATP concentrations in the RBC may be a key contributing factor for the acute effects of post exercise preconditioning and in the long term effects of program of regular exercise in cardiovascular protection.…”