Exercício físico promove neuroproteção estrutural e funcional em ratos com isquemia cerebral

Damázio, Laila Cristina Moreira; Melo, Raphael Teixeira Rocha; Lima, Maira de Castro; Pereira, Vanessa Guedes; Ribeiro, Rosy Iara Maciel de Azambuja; Alves, Natália Ribeiro de Magalhães; Monteiro, Betânia Souza; Natali, Antônio José; Carlo, Ricardo Junqueira Del; Maldonado, Izabel Regina dos Santos Costa

doi:10.34024/rnc.2015.v23.7978

Cited by 6 publications

(3 citation statements)

References 26 publications

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“…Moderate intensity physical exercise decreases cell apoptosis, increases the neurotrophic factors, and promotes mitochondrial biogenesis and angiogenesis (Chae and Kim, 2009;Chung et al, 2013). When physical exercise precedes cerebral ischemia, it promotes cerebral neuroprotection in rats, and among the mechanisms involved, we can highlight decreased inflammatory reaction, decreased cerebral edema, increased synthesis of brain-derived neurotrophic factor and neuronal growth factor (Damázio et al, 2015). According to Dornbos et al (2013), the greatest benefit of pre-ischemic exercise is the optimization of the ability to maintain the energy supply.…”

Section: Introductionmentioning

confidence: 99%

Research Article Lack of effect of exercise on the expression profiles of mir-16, mir-21, mir-155, caspase3 and bcl2 in a rat model of focal cerebral ischemia

Porsani¹,

Cirino²,

Neto³

et al. 2020

Genet. Mol. Res.

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Stroke is a major public health problem, with high mortality rates, and a high frequency of disability. Between 1990 and 2010, stroke increased from the fifth to the third leading cause of disability. In addition, its incidence has increased among younger people, with severe health consequences and increased social costs. There is evidence that physical exercise promotes neuroprotective effects when used as a therapeutic treatment. However, the mechanisms of neuroprotection are not yet well known. The objective of the study was to evaluate the expression profile of microRNAs miR-16, miR-21 and miR-155 and the CASPASE-3 and Bcl-2 genes previously related to apoptosis in the tissue (ischemic focus). Forty-eight Wistar rats were divided into four experimental groups: control group, ischemia group, ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 19 (4): gmr18594 L.B. Porsani et al. 2 exercise group and exercise + ischemia group. Before the ischemic procedure, the animals in the exercise and exercise + ischemia groups were submitted to a treadmill training protocol for four weeks. The training lasted 30 min a day at a speed of 18 m / min. For real-time PCR analysis, a fragment of the ischemic area was collected from each animal using a punch to analyze the expression of miRNAs; miR16, miR-21, miR-155, CASPASE-3 and Bcl-2 genes. In the animals that had physical exercise, there appeared to be a neuroprotective effect by the action of microRNAs and CASPASE-3, although no significant difference was observed. Further studies are needed to elucidate the role of apoptosis mechanisms in cerebral ischemia associated with physical exercise, as well as the role of microRNAs in the modulation of targets associated with this mechanism.

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Section: Introductionmentioning

confidence: 99%

Research Article Lack of effect of exercise on the expression profiles of mir-16, mir-21, mir-155, caspase3 and bcl2 in a rat model of focal cerebral ischemia

Porsani¹,

Cirino²,

Neto³

et al. 2020

Genet. Mol. Res.

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“…As adapted from Damázio et al (2015) and Uluç et al (2011), the brain was dissected and cut coronally, in slices of about 3 mm thick, which were submerged in a solution dissolved in PBS (0.1mol/L) in the 2% concentration of 2,3,5triphenyltetrazolium chloride (TTC) at 37ºC for 1 hour for marking areas of cerebral ischemia.…”

Section: Histological Evaluationmentioning

confidence: 99%

Oxygen-Ozone therapy for treatment of induced Ischemic Stroke in an >i<in vitro>/i< animal model

Orlandin¹

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“…A intensidade do exercício físico também influencia as alterações neuronais. O exercício físico de intensidade moderada diminui a apoptose celular, aumenta a presença de fatores neurotróficos, promove a biogênese mitocondrial e a angiogênese (CHAE;KIM, 2009;CHUNG et al, 2013) Nas situações em que o exercício físico antecede à isquemia cerebral; situação deste presente estudo; o mesmo promove neuroproteção cerebral em ratos e, entre os mecanismos envolvidos, podemos destacar diminuição da reação inflamatória, diminuição do edema cerebral, aumento da síntese do fator neurotrófico derivado do cérebro e do fator de crescimento neuronal (DAMÁZIO et al, 2015). otimização da capacidade de manutenção do suprimento de energia.…”

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O papel do exercício físico na apoptose em modelo de isquemia cerebral experimental

Porsani¹

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Introdução: O acidente vascular cerebral (AVC) é um grande problema de saúde pública, com altas taxas de mortalidade e altos níveis de incapacidade. Entre 1990 e 2010, o AVC aumentou da quinta para a terceira principal causa de incapacidade. Além disso, sua incidência tem aumentado entre pessoas mais jovens, situação em que existem sequelas significativas na saúde e aumento nos custos sociais. Há evidências de que o exercício físico promove efeitos neuroprotetores quando utilizado como tratamento terapêutico em modelo de isquemia experimental. Entretanto, os mecanismos de neuroproteção ainda não estão bem elucidados Objetivo: O objetivo deste estudo foi avaliar por PCR em tempo real o perfil de expressão dos microRNAs miR-21, miR-16, miR-155 no sangue e no tecido (foco isquêmico) em ratos, após a realização dos treinamentos físicos pré-isquemia cerebral focal. Materiais e Métodos: Foram utilizados 48 ratos da linhagem Wistar divididos em quatro grupos experimentais: grupo controle, grupo isquemia, grupo exercício e grupo exercício + isquemia. A metodologia de PCR em tempo real foi utilizada para analisar a expressão dos miRNAs; miR16, miR-21, miR-155, CASPASE-3 e Bcl-2. Resultados e conclusões: Em nosso estudo não observamos diferenças estatísticas significativas na expressão dos miRNAs: miR-16, miR-21, miR-155 e dos genes BCL-2 e CASPASE-3 no tecido cerebral nos grupos submetidos à isquemia cerebral e ao exercício físico quando comparados ao grupo controle.

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Exercício físico promove neuroproteção estrutural e funcional em ratos com isquemia cerebral

Cited by 6 publications

References 26 publications

Research Article Lack of effect of exercise on the expression profiles of mir-16, mir-21, mir-155, caspase3 and bcl2 in a rat model of focal cerebral ischemia

Research Article Lack of effect of exercise on the expression profiles of mir-16, mir-21, mir-155, caspase3 and bcl2 in a rat model of focal cerebral ischemia

Oxygen-Ozone therapy for treatment of induced Ischemic Stroke in an >i<in vitro>/i< animal model

O papel do exercício físico na apoptose em modelo de isquemia cerebral experimental

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