Excretion Pattern of Labelled Spermatozoa and the Timing of Spermatozoa Formation and Epididymal Transit in Rabbits Injected With Thymidine-3h

Amann, R. P.; Koefoed-Johnsen, H. H.; Levi, Haya

doi:10.1530/jrf.0.0100169

Cited by 35 publications

(15 citation statements)

References 8 publications

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“…The isotope labelling method, however, seems to give more consistent and reliable results (Orgebin-Crist, 1962). This method has been used for the mouse (Meistrich et al, 1975), bull (Koefoed-Johnsen, 1960 and rabbit (Amann et al, 1965;OrgebinCrist, 1965). The total passage times of spermatozoa through the rat epididymis obtained in the present study (8 days) are comparable to those estimated from the injection of radio-opaque material into the efferent ducts (7-8 days : Macmillan & Aukland, 1960) or calculated from the daily sperm reserve (8-4 days: Robb, Amann & Killian, 1978).…”

Section: Discussionmentioning

confidence: 99%

“…The animals were randomly assigned to 11 groups of 5-12 rats. The testicular spermatozoa were labelled using [3H]thymidine as previously described by others (KoefoedJohnsen, 1960;Amann, Koefoed-Johnsen & Levi, 1965;Orgebin-Crist, 1965;Meistrich et al, 1975) Ci/mmol; Amersham International pic, U.K.), dissolved in 0-2 ml 0-9% (w/v) sodium chloride solution, into both testes. The rats were then returned to their cages and kept for 35-52 days.…”

Section: Methodsmentioning

confidence: 99%

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Enhancement of sperm transport through the rat epididymis after castration

Sujarit¹,

Pholpramool²

1985

Reproduction

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Transport of spermatozoa through different regions of the epididymis has been followed by labelling testicular spermatozoa with [3H]thymidine in intact rats and in rats in which the efferent ducts were ligated or the testes were removed. In intact rats, the transit times of epididymal spermatozoa from the initial segment to the caput, from the caput to the corpus, and from the corpus to the cauda were 2, 4 and 2 days, respectively, giving a total transit time of 8 days. After bilateral castration, labelled spermatozoa were transferred from the initial segment into the proximal cauda by 2 days and appeared in the ductus deferens by 4 days. This effect was prevented by a daily subcutaneous injection of testosterone propionate (0.2 mg/kg). Bilateral efferent duct ligation had only a slight effect on the passage of epididymal spermatozoa. The results indicate that epididymal sperm transport is enhanced after androgen withdrawal.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Enhancement of sperm transport through the rat epididymis after castration

Sujarit¹,

Pholpramool²

1985

Reproduction

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“…The present study is the first quantitative measurement of the transport distances of the epididymal contents. The earlier data for the duration of epididymal transport are based on calculations of the size of the sperm reserves in different parts of the epididymis (Ortavant, 1959 ;Amann, Johnson, Thompson & Pickett, 1976) or on the measurement of the duration of transport through the major segments of the epididymis of germ cells labelled with radioactive precursors (Ortavant, 1954;Clermont, Leblond & Messier, 1959;Monesi, 1962;Amann, Koefoed-Johnsen & Levi, 1965;Kopecny, 1970;Loir, 1972;English & Dym, 1982). There was a large dispersion of the oil droplets, especially in the caput, indicating the stochastic nature of the epididymal transport (Jaakkola & Talo, 1982, 1983.…”

Section: Discussionmentioning

confidence: 99%

Regional variations in transport of the luminal contents of the rat epididymis in vivo

Jaakkola¹

1983

Reproduction

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The speed of transport of the luminal contents of the epididymis was studied by injecting small droplets of stained paraffin oil into the lumen in 10 different regions of the epididymal duct of 64 anaesthetized rats and measuring the distances the droplets moved in 2 h. The oil droplets became dispersed, and there was much variation between the animals. The dispersion distances were longest in the proximal caput and shortest in the corpus. The distributions of the transport distances of the oil droplets were discontinuous and multimodal in the proximal caput, and bimodal in the cauda. The transport speed decreased steeply from 420 mm/2 h in the most proximal caput to 64.2 mm/2 h in the beginning of the distal caput. In the rest of the caput, corpus and cauda, speed was quite similar although it declined slightly towards the ductus deferens, being 25.5 mm/2 h in the most distal region of the cauda.

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“…The normal time for spermatogenesis and transit to epididymal reserves is about 40 days [19]. Transit time from the testes through the epididymis is about 10 days [19, 20].…”

Section: Discussionmentioning

confidence: 99%

“…Transit time from the testes through the epididymis is about 10 days [19, 20]. Thus, the post reversal semen collections were performed after a minimum of 2.5 cycles of sperm production to ensure newly produced spermatozoa were being ejaculated.…”

Section: Discussionmentioning

confidence: 99%

Reversibility of Vasalgel™ male contraceptive in a rabbit model

Waller¹,

Bolick²,

Lissner³

et al. 2017

Basic Clin. Androl.

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BackgroundDevelopment of a non-hormonal long-acting reversible contraceptive for men could have a significant impact on reducing unintended pregnancies. Vasalgel™ is a high molecular weight polymer consisting of styrene-alt-maleic acid (SMA) dissolved in dimethyl sulfoxide being developed as a reversible male contraceptive device. It forms a hydrogel when implanted into the vasa deferentia, which prevents the passage of sperm. Previous studies in the rabbit have proven its efficacy, durability and rapid onset. This study evaluates the capacity to restore sperm concentrations in ejaculates after a reversal procedure.MethodsSodium bicarbonate was injected into the vasa deferentia after fourteen months of azoospermia following the injection of two device variations (Vasalgel 100 and Vasalgel 80). Semen samples were then collected for six months and sperm characteristics were compared to baseline levels. Samples of vasa deferentia were obtained for histological examination.ResultsSpermatozoa were present in all subject ejaculates after the reversal procedure. Sperm concentration and sperm motility were similar to baseline levels after reversal, while sperm forward progression was significantly lower and normal acrosomes were not observed. Forward progression percentages increased linearly during six months of semen collection, however, normal acrosomes were not observed at the conclusion of the study. Histologically, several vasa deferentia were clear of the device and contained an intact epithelial lining. A smaller proportion of tissues contained residual test material. A secondary intraluminal inflammatory response was seen occasionally in the tissues containing residual material. There was no difference between the two device variations for studied parameters.ConclusionsVasalgel’s prevention of sperm transport for 14 months was reversed through an intravasal injection of sodium bicarbonate. Post-reversal sperm concentrations and motility returned to baseline levels during the six-month follow up. Residual material in the vas lumen or compromised epididymal and vas deferens function may be resulting in reduced forward progression and loss of acrosomes during transit through the vas. Reduced forward progression and the lack of normal acrosomes strongly suggest impaired sperm function.

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Excretion Pattern of Labelled Spermatozoa and the Timing of Spermatozoa Formation and Epididymal Transit in Rabbits Injected With Thymidine-3h

Abstract: Summary. Sixteen

Cited by 35 publications

References 8 publications

Enhancement of sperm transport through the rat epididymis after castration

Enhancement of sperm transport through the rat epididymis after castration

Regional variations in transport of the luminal contents of the rat epididymis in vivo

Reversibility of Vasalgel™ male contraceptive in a rabbit model

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