Excretion of norsteroids’ phase II metabolites of different origin in human

Guay, Claudiane; Goudreault, Danielle; Schänzer, W.; Flenker, Ulrich; Ayotte, Christiane

doi:10.1016/j.steroids.2008.10.015

Cited by 13 publications

(18 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Interesting results were obtained for E glucuronide concentrations, which were elevated during the first trimester, and thus a feature to take into account in interpreting of T/E in steroid profiles of female athletes. The results of this study were also well in accordance with the earlier ones describing the formation of norandrosterone, a nandrolone metabolite, during pregnancy 11 52…”

Section: Abp and The Steroidal Modulesupporting

confidence: 92%

Confounding factors and genetic polymorphism in the evaluation of individual steroid profiling

2014

View full text Add to dashboard Cite

In the fight against doping, steroid profiling is a powerful tool to detect drug misuse with endogenous anabolic androgenic steroids. To establish sensitive and reliable models, the factors influencing profiling should be recognised. We performed an extensive literature review of the multiple factors that could influence the quantitative levels and ratios of endogenous steroids in urine matrix. For a comprehensive and scientific evaluation of the urinary steroid profile, it is necessary to define the target analytes as well as testosterone metabolism. The two main confounding factors, that is, endogenous and exogenous factors, are detailed to show the complex process of quantifying the steroid profile within WADA-accredited laboratories. Technical aspects are also discussed as they could have a significant impact on the steroid profile, and thus the steroid module of the athlete biological passport (ABP). The different factors impacting the major components of the steroid profile must be understood to ensure scientifically sound interpretation through the Bayesian model of the ABP. Not only should the statistical data be considered but also the experts in the field must be consulted for successful implementation of the steroidal module.

show abstract

Section: Abp and The Steroidal Modulesupporting

confidence: 92%

Confounding factors and genetic polymorphism in the evaluation of individual steroid profiling

2014

View full text Add to dashboard Cite

show abstract

“…There was a clear increase in 19-NA throughout the pregnancy, as has been demonstrated before, 11,13 but none of the pregnant women reached the cut-off of 15 ng/mL. This indicates that pregnant women can use low doses of 19-norsteroids without being identified as atypical findings.…”

Section: Discussionsupporting

confidence: 55%

“…12 So far, there are no population-based studies of how 19-NA varies throughout the pregnancy. 13,14 If the concentration of 19-NA is found to be greater than 2.5 ng/mL in a sample from a female athlete, a pregnancy test is performed. 13,14 If the concentration of 19-NA is found to be greater than 2.5 ng/mL in a sample from a female athlete, a pregnancy test is performed.…”

Section: Introductionmentioning

confidence: 99%

Pregnancy greatly affects the steroidal module of the Athlete Biological Passport

Mullen

Gadot

Eklund

et al. 2018

Drug Testing and Analysis

View full text Add to dashboard Cite

Concentrations of urinary steroids are measured in anti-doping test programs to detect doping with endogenous steroids. These concentrations are combined into ratios and followed over time in the steroidal module of the Athlete Biological Passport (ABP). The most important ratio in the ABP is the testosterone/epitestosterone (T/E) ratio but this ratio is subject to intra-individual variations, especially large in women, which complicates interpretation. In addition, there are other factors affecting T/E. Pregnancy, for example, is known to affect the urinary excretion rate of epitestosterone and hence the T/E ratio. However, the extent of this variation and how pregnancy affect other ratios has not been fully evaluated. Here we have studied the urinary steroid profile, including 19-norandrosterone (19-NA), in 67 pregnant women and compared to postpartum. Epitestosterone was higher and, consequently, the T/E and 5αAdiol/E ratios were lower in the pregnant women. Androsterone/etiocholanolone (A/Etio) and 5αAdiol/5βAdiol, on the other hand, were higher in the first trimester as compared to postpartum (p<0.0001 and p=0.0396, respectively). There was no difference in A/T during pregnancy or after. 19-NA was present in 90.5% of the urine samples collected from pregnant women. In this study, we have shown that the steroid profile of the ABP is affected by pregnancy, and hence can cause atypical passport findings. These atypical findings would lead to unnecessary confirmation procedures, if the patterns of pregnancy are not recognized by the ABP management units.

show abstract

“…This supported the findings of studies reviewed previously relating to DHEA and 5-ADIOL. [16,48,71,81] The potential for discrete glucuronide and sulfoconjugate excretion of 19-nor steroids to provide evidence of origin was investigated by Guay et al [127] and Strahm et al [128] who concluded that IRMS was required to confirm any abnormal metabolic flux observations. On a pharmacokinetic level, these studies demonstrated that sulfoconjugation is predominant at low substrate concentration whereas glucuronidation is the major conjugation process at high substrate levels because of saturation kinetics affecting the sulfation reaction.…”

Section: -Nor Steroidsmentioning

confidence: 99%

Complementary stable carbon isotope ratio and amount of substance measurements in sports anti‐doping

Cawley

George

2012

Drug Testing and Analysis

View full text Add to dashboard Cite

The detection of steroids originating from synthetic precursors against a background of their chemically identical natural analogues has proven to be a significant challenge for doping control laboratories accredited by the World Anti‐Doping Agency (WADA). The complementary application of gas chromatography–mass spectrometry (GC‐MS) and gas chromatography‐combustion‐isotope ratio mass spectrometry (GC‐C‐IRMS) has been demonstrated to provide specific detection of endogenous steroid misuse for improved anti‐doping analysis. Markers of synthetically derived steroids are reviewed on the basis of abnormal urinary excretions and low 13C content. A combinatorial approach is presented for the interpretation of GC‐MS and GC‐C‐IRMS data in the anti‐doping context. This methodology can allow all relevant information concerning an individual's metabolism to be assessed in order to make an informed decision with respect to a doping violation. Copyright © 2012 John Wiley & Sons, Ltd.

show abstract

Excretion of norsteroids’ phase II metabolites of different origin in human

Cited by 13 publications

References 45 publications

Confounding factors and genetic polymorphism in the evaluation of individual steroid profiling

Confounding factors and genetic polymorphism in the evaluation of individual steroid profiling

Pregnancy greatly affects the steroidal module of the Athlete Biological Passport

Complementary stable carbon isotope ratio and amount of substance measurements in sports anti‐doping

Contact Info

Product

Resources

About