Exclusive Production of Gentamicin C1a from Micromonospora purpurea by Metabolic Engineering

Zeng, Wei; Shi, Xiaoyan; Lian, Rong; Wang, Weibin; Hong, Wei; Guo, Shaobin

doi:10.3390/antibiotics8040267

Cited by 9 publications

(7 citation statements)

References 19 publications

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“…supported their own vital functions. The known factors of lysis of erythrocyte cultures microsporous fungi Micromonospora purpurea/Micromonospora echinospora (gentamicin producers) had a negative impact on the procedural links of cultural method [9].…”

Section: Resultsmentioning

confidence: 99%

Cultural Method in Babesiosis Pathogens Diagnosis: Current State of the Problem

Torianyk¹

2021

Wiad Lek

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The aim is to show the effectiveness of the cultural method in the diagnosis of babesiosis. Materials and methods: The study material was the blood from 10 healthy humans and animals, 30 humans with borreliosis, 41 animals with babesiosis. A cultural research method was used. Results: The use of cultural method contributed to the optimization of the etiopathogenetic diagnosis of babesiosis. The method objectively proved the ability of protozoa (Babesia spp.) to asexual reproduction in vitro on the multicomponent growth medium with erythrocytes; proved the role of erythrocytes as acceptable for Babesia spp. of target cells. Absolute and relative parameters of detection of Babesia spp. by cultural method in the blood samples of the groups of the examined persons/species were different, making on average 19.7% (with the highest rates among the domestic dogs (62.5%) and cattle (20.0%)). Negative result (0%) was identified among the patients with borreliosis. Conclusions: Detection of Babesia spp. by the cultural method objectively confirmed its value as an apparent laboratory criterion for the etiopathogenetic diagnosis of babesiosis.

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Section: Resultsmentioning

confidence: 99%

Cultural Method in Babesiosis Pathogens Diagnosis: Current State of the Problem

Torianyk¹

2021

Wiad Lek

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“…In addition, Intestinimonas and its positively associates metabolites Gentamicin C1a, Selenodiglutathione, L-Arabinose, Serotonin, Norfloxacin, 2-Oxoarginine, Epinephrine, and Xanthine were increased in the gut of chicken that treated with BAI compared to the CON chicken. Gentamicin C1a is considered as a precursor to the synthesis of etimicin (Wei et al, 2019). Selenodiglutathione is a primary Se metabolite conjugated to two glutathione moieties, and it increase intracellular Se accumulation and is more toxic than selenous acid (Fisher et al, 2016;Tobe et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Baicalin protects against APEC-induced colibacillosis by regulating gut microbiota and its metabolites in Chickens

Peng¹,

Shi²,

Gong³

et al. 2020

Preprint

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BACKGROUND AND PURPOSE Chicken colibacillosis, caused by avian Escherichia coli (APEC), results in huge economic losses to the poultry industry. Baicalin exerts protective effects during the development of colibacillosis. In this study, we mainly explored the mechanism of this protective effects with regard to gut microbiota. EXPERIMENTAL APPROACH The chicken colibacillosis model was established by intratracheal instillation of APEC. The gut microbiota-depleted chicken model was established with broad-spectrum antibiotics. Viscera index measurement and Haematoxylin and eosin stain were applied to assess histological changes of tissues. ELISA were used to measure the cytokines and Quantitative-PCR were used to evaluate the gene expression. The gut microbiota and its metabolite were detected by 16srDNA and ultrahigh-performance liquid chromatography (LC-MS). KEY RESULTS Depletion of gut microbiota exacerbated the tissue damage and weakened the protective effects of baicalin during APEC-induced chicken colibacillosis while pretreatment of baicalin reduced these changes and inflammatory response induced by APEC. Moreover, APEC infection led to dysbiosis of gut microbiota and its metabolites. However, the pretreatment of baicalin remodeled the gut microbiota featured with increased abundance of Intestinimonas and its associated with beneficial metabolites. CONCLUSIONS Gut microbiota played a protective role in the prevention of chicken colibacillosis and the pharmacological action of baicalin. The altered specific gut bacterial and/or metabolites may be served as indicators to predict the occurrence and prognosis of chicken colibacillosis. Our findings may provide a paradigm for the mechanistic studies of compounds and aid the exploration of the mechanisms and pathways underlying the function of herbal medicines.

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“…Aminoglycosides are mostly ineffective against anaerobic bacteria, fungi, and viruses, typically used for moderate to severe gram-negative infections. It may cause ear and kidney damage 20 .…”

Section: Gentamicinmentioning

confidence: 99%

Untitled

2022

IJPSR

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Infectious diseases are the most significant cause of fatalities worldwide. Staphylococcus aureus could be a common human infective microorganism that will trigger various infectious diseases, like soft tissue and skin infections, osteomyelitis, bacteremia, endocarditis, and fatal respiratory illnesses. In recent decades, because of the evolution of microorganisms and the abuse of antibiotics, the drug resistance of S. aureus has been stepped by step exaggerated, and the infection rate has risen worldwide. Accumulating proof has demonstrated that the resistance mechanisms of S. aureus are complicated, that is, resistant to several types of antibiotics. Multiple antibiotic-resistant Staphylococcus aureus is one of the common causes of severe nosocomial infections, and the gastrointestinal tract is an important source of its transmission. Productive treatment remains difficult and needs the analysis of each novel anti-microbial and connected aspect of care, like communicable disease consultation, diagnostic technique and supply source. This review focuses on developing resistance to currently used antibiotics and examines prospects for new antibiotics and the known use of drug combos.INTRODUCTION: India carries one of the biggest burdens of drug-resistant pathogens worldwide. In 2008, NDM-1 reported that antimicrobial resistance had spread speedily to different countries. The Indian market is one of the biggest customers of antibiotics worldwide, and the global antibiotic trade is increasing speedily. Antimicrobial resistance arises once microbes develop a mechanism to escape the action of anti-microbial agents.

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Exclusive Production of Gentamicin C1a from Micromonospora purpurea by Metabolic Engineering

Cited by 9 publications

References 19 publications

Cultural Method in Babesiosis Pathogens Diagnosis: Current State of the Problem

Cultural Method in Babesiosis Pathogens Diagnosis: Current State of the Problem

Baicalin protects against APEC-induced colibacillosis by regulating gut microbiota and its metabolites in Chickens

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