Excited-State Kinetics of the Hydrophobic Probe Nile Red in Membranes and Micelles

Krishna, M. Bala

doi:10.1021/jp984620m

Cited by 125 publications

(92 citation statements)

References 32 publications

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“…Further con®rmation for these locations comes from the¯uorescence lifetime data (Table 2), which were single exponential in pure alkane, but which required multiexponential ®ts for both pure surfactant and the bicontinuous phase. The latter showed a pronounced wavelength dependence, similar to what has been reported for this probe in viscous solutions and micelles [9]. Whilst detailed analysis of this behaviour is beyond the scope of this work, the results lend support to different sites for the probe.…”

Section: Resultssupporting

confidence: 85%

Solvatochromic fluorescent probes in bicontinuous microemulsions

Oliveira

Hungerford

Miguel

2001

Journal of Molecular Structure

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The¯uorescence probes nile red and prodan have been used to study the structure of the bicontinuous phase of C 12 E 5 /alkane/ water microemulsions. For nile red the steady state¯uorescence spectrum show contributions from probe located in alkane and surfactant rich regions, together with indications of surfactant hydration. Fluorescence lifetime measurements show multiexponential decay kinetics consistent with this idea. The steady state¯uorescence spectrum of prodan in the bicontinuous region also suggests different environments for the probe. Fluorescence anisotropy measurements using rhodamine 6G show that this bicontinuous phase is more ordered than either oil in water or water in oil microemulsions. The¯uorescence technique provides a valuable tool with which to elucidate the structure of these complex systems. q

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Section: Resultssupporting

confidence: 85%

Solvatochromic fluorescent probes in bicontinuous microemulsions

Oliveira

Hungerford

Miguel

2001

Journal of Molecular Structure

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“…1). The time-resolved values have been linked to the viscosity of the environment and the formation of a solvent relaxed state, with the presence of a biexponential decay and rise times noted [28]. Our data appear consistent with that model, although the need to use the sum of three exponentials indicates that our system is more complex than that previously reported [28].…”

Section: Resultssupporting

confidence: 83%

“…The decays are multiexponential and in general the average lifetimes are 60% those obtained at room temperature, showing the increased influence of non-radiative processes. The trend with increasing Ch concentration is for an increase in decay time after a slight dip on the initial addition of Ch, which reflects the trend in the rotational correlation time and is in agreement with the viscosity dependence of the fluorescence decay time [28].…”

Section: Resultssupporting

confidence: 80%

“…The time-resolved values have been linked to the viscosity of the environment and the formation of a solvent relaxed state, with the presence of a biexponential decay and rise times noted [28]. Our data appear consistent with that model, although the need to use the sum of three exponentials indicates that our system is more complex than that previously reported [28]. It should be noted that at room temperature these vesicles are principally in the gel phase and cholesterol apparently distributes evenly between the DODAB lipids, until ∼20%, when Ch enrichment leads to complex formation [15].…”

Section: Resultsmentioning

confidence: 99%

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Domain Formation in DODAB–Cholesterol Mixed Systems Monitored via Nile Red Anisotropy

et al. 2005

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The effect of the cholesterol (Ch) on liposomes composed of the cationic lipid dioctadecyldimethylammonium bromide (DODAB) was assessed by studying both the steady-state and time-resolved fluorescence anisotropy of the dye Nile Red. The information obtained combined with analysis of the steady-state emission and fluorescence lifetime of Nile Red (NR) for different cholesterol concentrations (5-50%) elucidated the presence of "condensed complexes" and cholesterol-rich domains in these mixed systems. The steady-state fluorescence spectra were decomposed into the sum of two lognormal emissions, emanating from two different states, and the effect of temperature on the anisotropy decay of Nile Red for different cholesterol concentrations was observed. At room temperature, the time-resolved anisotropy decays are indicative of NR being relatively immobile (manifest by a high r ∞ value). At higher temperature, rotational times ca. 1 ns were obtained throughout and a trend in increasing hindrance was seen with increase of Ch content.

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“…Many experimental techniques like NMR [9][10][11] along with time resolved fluorescence spectroscopy [12][13][14][15] and neutron scattering [11,16] have been used to study the structure and dynamics of hydration water. Study of water at protein surfaces [17][18][19][20], water next to DNA and RNA [21] were also performed.…”

Section: Introductionmentioning

confidence: 99%

Insights into the behavioral difference of water in the presence of GM1

2015

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Edited by A. ChattopadhyayKeywords: GM1 Rough surface Behavioral difference of water Structure Dynamic Delayed convergence of interfacial property to bulk property a b s t r a c t Studies on the structure and dynamics of interfacial water, emphasizing on the properties of water near the surface of biomolecules, are well reported, but there is a lack of evidence on the behavior of water near a comparatively rough surface containing molecules with a bulky head group like GM1.In this report we comparatively analyze the structure and dynamics of water as a function of distance from the lipid head group in GM1 containing lipid bilayers, with the lipid bilayers where GM1 is not present. This approach effectively demonstrates the behavioral difference and hence delayed convergence from bound water to bulk water in the presence of GM1 compared to a relatively smooth surface.

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Excited-State Kinetics of the Hydrophobic Probe Nile Red in Membranes and Micelles

Cited by 125 publications

References 32 publications

Solvatochromic fluorescent probes in bicontinuous microemulsions

Solvatochromic fluorescent probes in bicontinuous microemulsions

Domain Formation in DODAB–Cholesterol Mixed Systems Monitored via Nile Red Anisotropy

Insights into the behavioral difference of water in the presence of GM1

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