“…2). The observed time‐dependent extracellular decrease of these neurotransmitters in the control group was in support of previous observations involving urethane‐anesthetized rats (Men and Matsui, 1994; Keck et al, 2002) whereby the administration of urethane is known to suppress striatal neuronal activity and basal DA concentrations moderately (Warenycia and McKenzie, 1988; Hamilton et al, 1992; West, 1998) as well as serotonergic neurotransmission (Dringenberg and Vanderwolf, 1995). It is also notable that the direct sonication to the microdialysis probe resulted in a marked increase in the in vitro recovery rate, which potentially confounds the reliability of the level of dialysis.…”
Regional modulation of the level of cortical neurotransmitters in the brain would serve as a new functional brain mapping technique to interrogate the neurochemical actions of the brain. We investigated the utility of the application of low-intensity, pulsed sonication of focused ultrasound (FUS) to the brain to modulate the extracellular level of dopamine (DA) and serotonin (5-HT). FUS was delivered to the thalamic areas of rats, and extracellular DA and 5-HT were sampled from the frontal lobe using the microdialysis technique. The concentration changes of the sampled DA and 5-HT were measured through high-performance liquid chromatography. We observed a significant increase of the extracellular concentrations of DA and 5-HT in the FUS-treated group as compared with those in the unsonicated group. Our results provide the first direct evidence that FUS sonication alters the level of extracellular concentration of these monoamine neurotransmitters and has a potential modulatory effect on their local release, uptake, or degradation. Our findings suggest that the pulsed application of FUS offers new perspectives for a possible noninvasive modulation of neurotransmitters and may have diagnostic as well as therapeutic implications for DA/5-HT-mediated neurological and psychiatric disorders.
“…2). The observed time‐dependent extracellular decrease of these neurotransmitters in the control group was in support of previous observations involving urethane‐anesthetized rats (Men and Matsui, 1994; Keck et al, 2002) whereby the administration of urethane is known to suppress striatal neuronal activity and basal DA concentrations moderately (Warenycia and McKenzie, 1988; Hamilton et al, 1992; West, 1998) as well as serotonergic neurotransmission (Dringenberg and Vanderwolf, 1995). It is also notable that the direct sonication to the microdialysis probe resulted in a marked increase in the in vitro recovery rate, which potentially confounds the reliability of the level of dialysis.…”
Regional modulation of the level of cortical neurotransmitters in the brain would serve as a new functional brain mapping technique to interrogate the neurochemical actions of the brain. We investigated the utility of the application of low-intensity, pulsed sonication of focused ultrasound (FUS) to the brain to modulate the extracellular level of dopamine (DA) and serotonin (5-HT). FUS was delivered to the thalamic areas of rats, and extracellular DA and 5-HT were sampled from the frontal lobe using the microdialysis technique. The concentration changes of the sampled DA and 5-HT were measured through high-performance liquid chromatography. We observed a significant increase of the extracellular concentrations of DA and 5-HT in the FUS-treated group as compared with those in the unsonicated group. Our results provide the first direct evidence that FUS sonication alters the level of extracellular concentration of these monoamine neurotransmitters and has a potential modulatory effect on their local release, uptake, or degradation. Our findings suggest that the pulsed application of FUS offers new perspectives for a possible noninvasive modulation of neurotransmitters and may have diagnostic as well as therapeutic implications for DA/5-HT-mediated neurological and psychiatric disorders.
“…Rats were anaesthetized with a-chloralose (50 mg/kg) and urethane (500 mg/kg), both in 0.9% NaCl made in distilled water, and given intraperitoneally. Dexamphetamine is still able to excite striatal neurones (Warenycia & McKenzie 1988). The use of urethane-chloralose anaesthesia requires some comments.…”
In vivo microdialysis was used to examine the effects of tolcapone (30 mg/kg) on dopamine metabolism in amphetamine (5 mg/kg) and pargyline (75 mg/kg) treated rats. Amphetamine- or pargyline-induced decreases in the extracellular dihydroxyphenyl acetic acid (DOPAC) levels were counteracted by additional tolcapone. Tolcapone also decreased homovanillic acid effluxes below those caused by amphetamine or pargyline. However, dopamine effluxes were not further enhanced by additional tolcapone. These results show that central metabolism of dopamine can be modulated by catechol-O-methyltransferase (COMT) inhibition also without exogenous L-DOPA. However, extracellular dopamine levels are not easily increased.
“…However, α-chloralose as well as urethane (which is widely used for non-recovery electrophysiological studies) alters noradrenaline release from the paraventricular nucleus of the hypothalamus, which may affect autonomic and endocrine function (Shimokawa et al 1998). Urethane and chloral hydrate may disturb serotonergic function (Dringenberg and Vanderwolf 1995) and have been demonstrated to reduce striatal neuronal activity and basal dopamine levels but enhance the neuronal response to dexamphetamine (Warenycia and McKenzie 1988;Hamilton et al 1992). Urethane and chloral hydrate decrease cocaineinduced Fos expression in the striatum via a glutamatergic mechanism, with no effects on cocaine-induced increases in dopamine levels (Kreuter et al 2004).…”
Section: Effects Of General Anaesthetics On Neurotransmitter Systemsmentioning
Experimental and analytical considerations, including anaesthesia, physiological monitoring, drug dose and delivery, scanning protocols, statistical approaches and the interpretation of phMRI data, are discussed.
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