Excisions of a defective transposable CACTA element (Tetu1) generate new alleles of a CYCLOIDEA-like gene of Helianthus annuus

Fambrini, Marco; Basile, Alice; Salvini, Mariangela; Pugliesi, Claudio

doi:10.1016/j.gene.2014.07.018

Cited by 20 publications

(47 citation statements)

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“…This CACTA alters the transcriptional activity of HaCYC2c, in which the expression extends into the inflorescence, suggesting that the insertion of the TE is an essential step to generate the Chry phenotype. In contrast, when the basal region of the bHLH TCP motif of the HaCYC2c gene is interrupted by the insertion of the incomplete CACTA transposon Tetu1, the ray flowers are transformed from zygomorphic to actinomorphic, assuming a resemblance to the disk flowers [20,21,32]. In this mutant, (turf ) the ray flowers also recover their hermaphroditic features, developing both male and female reproductive organs.…”

Section: Discussionmentioning

confidence: 99%

“…Putative CACTA elements were isolated from the HanXRQr1.0 version of the sunflower genome sequence [24], deposited at the NCBI site (https://www.ncbi.nlm.nih.gov/) (WGS project number PRJNA396063). Putative CACTA sequences were isolated by using full-length elements retrieved by Badouin et al [24] along with the sequence of Tetu1 [20,21] as a query for a BLASTN search (-E-value 1e-10). This approach was found to be adequate for this type of particularly heterogeneous sequence.…”

Section: Sequence Collectionmentioning

confidence: 99%

“…Previous studies showed that a spontaneous insertion of a non-autonomous CACTA-like element ( Figure 1C), known as the transposable element of turf1 (Tetu1), in the coding region of the HaCYC2c gene generates the turf mutant [20]. In particular, the insertion of Tetu1 in the TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING NUCLEAR ANTIGEN CELL FACTOR (TCP) 1 and 2 motif of the turf-HaCYC2c gene changes the reading frame for the encoded protein, inserting a premature stop codon in the sequence [20][21][22]. In sunflower, a similar phenotype, called tubular-rayed, caused by the insertion of a Class I TE in the HaCYC2c gene, was also described [23].…”

Section: Introductionmentioning

confidence: 99%

“…The DNA TEs were estimated to represent less than 3% of the nuclear DNA, whereas the CACTA superfamily represents about 0.13% [26]. Notably, only a few active CACTA elements were identified in sunflower [20,21,23].…”

Section: Introductionmentioning

confidence: 99%

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On the Trail of Tetu1: Genome-Wide Discovery of CACTA Transposable Elements in Sunflower Genome

Ventimiglia

Pugliesi

Vangelisti

et al. 2020

IJMS

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Much has been said about sunflower (Helianthus annuus L.) retrotransposons, representing the majority of the sunflower’s repetitive component. By contrast, class II transposons remained poorly described within this species, as they present low sequence conservation and are mostly lacking coding domains, making the identification and characterization of these transposable elements difficult. The transposable element Tetu1, is a non-autonomous CACTA-like element that has been detected in the coding region of a CYCLOIDEA (CYC) gene of a sunflower mutant, tubular ray flower (turf). Based on our knowledge of Tetu1, the publicly available genome of sunflower was fully scanned. A combination of bioinformatics analyses led to the discovery of 707 putative CACTA sequences: 84 elements with complete ends and 623 truncated elements. A detailed characterization of the identified elements allowed further classification into three subgroups of 347 elements on the base of their terminal repeat sequences. Only 39 encode a protein similar to known transposases (TPase), with 10 TPase sequences showing signals of activation. Finally, an analysis of the proximity of CACTA transposons to sunflower genes showed that the majority of CACTA elements are close to the nearest gene, whereas a relevant fraction resides within gene-encoding sequences, likely interfering with sunflower genome functionality and organization.

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Section: Discussionmentioning

confidence: 99%

Section: Sequence Collectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

On the Trail of Tetu1: Genome-Wide Discovery of CACTA Transposable Elements in Sunflower Genome

Ventimiglia

Pugliesi

Vangelisti

et al. 2020

IJMS

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“…Instead of mainly expressed in the dorsal portion to establish bilateral symmetry of individual flower, CYC -like proteins in Asteraceae participate in regulating the identity of flower types in inflorescence (Broholm et al, 2008; Kim et al, 2008; Juntheikki-Palovaara et al, 2014). In sunflower, the turf or tub mutants are characterized by a shift from zygomorphic to actinomorphic ray floret, due to insertion of transposable elements in the HaCYC2c TCP motif that leads to a premature stop codon (Fambrini et al, 2011, 2014a; Chapman et al, 2012). In contrast, in the dbl or chry mutants, caused by an insertion upstream the coding region, HaCYC2c is expressed throughout the flower head, converting DF into ray-like ones (Chapman et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of CYC-Like Genes in Regulation of Ray Floret Development in Chrysanthemum morifolium

Huang

Sun

et al. 2016

Front. Plant Sci.

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Chrysanthemum morifolium, one of the most economically important ornamental crops worldwide, is well-known for the elaborate and complex inflorescence which is composed of both bilaterally symmetrical ray florets and radially symmetrical disc florets. Despite continuing efforts, the molecular mechanisms underlying regulation of the two flower types are still unclear so far. CYC-like proteins have been shown to control flower symmetry or regulate flower-type identity in several angiosperm plant lineages. In this study, we conducted comparative analysis of the CmCYC2 genes in two chrysanthemum cultivars and their F1 progenies with various whorls of ray florets. Six CmCYC genes were identified and sequenced, all of which were grouped into the CYC2 subclade. All the six CmCYC2 genes were predominantly expressed in reproductive organs, and in particular in the petal of ray florets. Of these genes, the transcription level of CmCYC2c was highly up-regulated in ray florets of the double-ray flowered heads. In addition, the result that CmCYC2c was highly expressed at key developing stages indicates its role in regulating petal development. Furthermore, overexpression of CmCYC2c in C. lavandulifolium, one of the original species of C. morifolium, led to significant increase in flower numbers and petal ligule length of ray florets. Besides CmCYC2c, the expression of CmCYC2f was also significantly up-regulated in transgenic lines, implying a possible role in regulating development of ray florets. Both results of expression patterns and transgenic phenotypes suggest that CmCYC2c is involved in regulating ray floret identity in the chrysanthemum. This study will be useful for genetic manipulation of flower shape in chrysanthemum and hence promote the process of molecular breeding.

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The plastic genome: The impact of transposable elements on gene functionality and genomic structural variations

Fambrini

Usai

Vangelisti

et al. 2020

Genesis

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Transposable elements (TEs) are DNA sequences that can change their position within genomes. TEs are present in most organisms and can be an important genomic component. Their activities are manifold: restructuring of genome size, chromosomal rearrangements, induction of gene mutations, and alteration of gene activity by insertion near or within promoters, intronic regions, or enhancer. There are several examples of mutations and other genetic variations determined by the activity of TEs, associated with the evolution of prokaryotic and eukaryotic organisms and the domestication of plants. Generally, TE mobilization occurs when the organism is subjected to stress, which can include both biotic and abiotic stresses, polyploidy conditions, and interspecific hybridizations, very common events in plants. TEs are widely distributed among organisms. TEs also play essential roles in evolution, but most of them are either dormant or inactive. This is mainly determined by epigenetic silencing mechanisms, regulatory systems, and control systems that aim to limit its proliferation. Furthermore, the host has recruited many genes originated from TEs as transcriptional regulators, especially in defense against pathogens and invasive genetic elements; this phenomenon is called molecular domestication. Therefore, TEs are responsible for horizontal gene transfer and the movement of genetic material between organisms, even phylogenetically distant, with a consequent remixing of their gene pools.

show abstract

Excisions of a defective transposable CACTA element (Tetu1) generate new alleles of a CYCLOIDEA-like gene of Helianthus annuus

Cited by 20 publications

References 70 publications

On the Trail of Tetu1: Genome-Wide Discovery of CACTA Transposable Elements in Sunflower Genome

On the Trail of Tetu1: Genome-Wide Discovery of CACTA Transposable Elements in Sunflower Genome

Identification and Characterization of CYC-Like Genes in Regulation of Ray Floret Development in Chrysanthemum morifolium

The plastic genome: The impact of transposable elements on gene functionality and genomic structural variations

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