Excision of the polydnavirus chromosomal integrated EP1 sequence of the parasitoid wasp Cotesia congregata (Braconidae, Microgastinae) at potential recombinase binding sites.

Savary, Stéphane; Beckage, Nancy E.; Tan, Frances F.; Périquet, Georges; Drezen, Jean‐Michel

doi:10.1099/0022-1317-78-12-3125

Cited by 72 publications

(98 citation statements)

References 29 publications

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“…First viral DNA sequences have to be amplified within wasp cells in nuclear virus factories. Contrary to initial hypotheses [44,45], it is not the circles themselves that are amplified from proviral segment excision but larger molecules [46] that are replicated linearly [36]. In C. congregata, two segments were found to amplify together within the same molecule as well as sequences not packaged in the particles [46].…”

Section: (Iii) Bracovirus Replicationmentioning

confidence: 69%

When parasitic wasps hijacked viruses: genomic and functional evolution of polydnaviruses

Herniou

Huguet

Thézé

et al. 2013

Phil. Trans. R. Soc. B

149

170

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International audienceThe Polydnaviridae (PDV), including the Bracovirus (BV) and Ichnovirus genera, originated from the integration of unrelated viruses in the genomes of two parasitoid wasp lineages, in a remarkable example of convergent evolution. Functionally active PDVs represent the most compelling evolutionary success among endogenous viral elements (EVEs). BV evolved from the domestication by braconid wasps of a nudivirus 100 Ma. The nudivirus genome has become an EVE involved in BV particle production but is not encapsidated. Instead, BV genomes have co-opted virulence genes, used by the wasps to control the immunity and development of their hosts. Gene transfers and duplications have shaped BV genomes, now encoding hundreds of genes. Phylogenomic studies suggest that BVs contribute largely to wasp diversification and adaptation to their hosts. A genome evolution model explains how multidirectional wasp adaptation to different host species could have fostered PDV genome extension. Integrative studies linking ecological data on the wasp to genomic analyses should provide new insights into the adaptive role of particular BV genes. Forthcoming genomic advances should also indicate if the associations between endoparasitoid wasps and symbiotic viruses evolved because of their particularly intimate interactions with their hosts, or if similar domesticated EVEs could be uncovered in other parasites

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Section: (Iii) Bracovirus Replicationmentioning

confidence: 69%

When parasitic wasps hijacked viruses: genomic and functional evolution of polydnaviruses

Herniou

Huguet

Thézé

et al. 2013

Phil. Trans. R. Soc. B

149

170

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“…Proviral segments were identified by comparison with circle sequences [19] and by the MEME/MAST program suite [20], which allowed extensive search of conserved segment extremities (DRJ) that have been shown to terminate bracovirus proviral segments [12]. The genuine presence of newly identified circles in the particles (S16, S24, S27, S28 and S29) was assessed by circle junction PCR tests, as each proviral segment extremity is joined in the circle.…”

Section: Materials and Methods (A) Insects And Dna Extractionmentioning

confidence: 99%

“…All proviral segments from all BVs analysed to date are terminated by direct repeats at both extremities, termed DRJs [12,13,16,29,30,32]. Bracovirus circles contain a unique sequence (circle junction) produced from a recombination event between these DRJs [12,33].…”

Section: (C) Proviral Segment Extremities Are Conservedmentioning

confidence: 99%

“…Bracovirus circles contain a unique sequence (circle junction) produced from a recombination event between these DRJs [12,33]. Site-specific tyrosine recombinases identified in the nudiviral machinery (VLF-1a or VLF-1b) were proposed to perform this recombination [7] based on functional homology with the homologous baculovirus protein VLF-1 [34].…”

Section: (C) Proviral Segment Extremities Are Conservedmentioning

confidence: 99%

“…Previous studies using molecular approaches [12,13] and in situ hybridization on wasp chromosomes [14] showed that the proviral segments analysed were clustered together. This led to the hypothesis that all proviral segments might be organized in the wasp genome in tandem arrays constituting a macrolocus [14,15].…”

Section: Introductionmentioning

confidence: 99%

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Functional endogenous viral elements in the genome of the parasitoid wasp Cotesia congregata : insights into the evolutionary dynamics of bracoviruses

Bézier

Louis

Jancek

et al. 2013

Phil. Trans. R. Soc. B

151

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International audienceBracoviruses represent the most complex endogenous viral elements (EVEs) described to date. Nudiviral genes have been hosted within parasitoid wasp genomes since approximately 100 Ma. They play a crucial role in the wasp life cycle as they produce bracovirus particles, which are injected into parasitized lepidopteran hosts during wasp oviposition. Bracovirus particles encapsidatemultiple dsDNAcircles encoding virulence genes. Their expression in parasitized caterpillars is essential for wasp parasitism success. Here, we report on the genomic organization of the proviral segments (i.e. master sequences used to produce the encapsidated dsDNA circles) present in the Cotesia congregata parasitoid wasp genome. The provirus is composed of a macrolocus, comprising two-thirds of the proviral segments and of seven dispersed loci, each containing one to three segments. Comparative genomic analyses with closely related species gave insights into the evolutionary dynamics of bracovirus genomes. Conserved synteny in the differentwasp genomes showed the orthology of the proviral macrolocus across different species. The nudiviral gene odv-e66-like1 is conserved within themacrolocus, suggesting an ancient co-localization of the nudiviral genome and bracovirus proviral segments. By contrast, the evolution of proviral segments within the macrolocus has involved a series of lineage-specific duplications

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The few virus‐like genes of Cotesia congregata bracovirus

Drezen

Bézier

Lesobre

et al. 2006

Arch Insect Biochem Physiol

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The origin of the symbiotic association between parasitoid wasps and bracoviruses is still unknown. From phylogenetic analyses, bracovirus-associated wasp species constitute a monophyletic group, the microgastroid complex. Thus all wasp-bracovirus associations could have originated from the integration of an ancestral virus in the genome of the ancestor of the microgastroids. In an effort to identify a set of virus genes that would give clues on the nature of the ancestral virus, we have recently performed the complete sequencing of the genome of CcBV, the bracovirus of the wasp Cotesia congregata. We describe here the putative proteins encoded by CcBV genome having significant similarities with sequences from known viruses and mobile elements. The analysis of CcBV gene content does not lend support to the hypothesis that bracoviruses originated from a baculovirus. Moreover, no consistent homology was found between CcBV genes and any set of genes constituting the core genome of a known free-living virus. We discuss the significance of the scarce homology found between proteins from CcBV and other viruses or mobile elements.

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Excision of the polydnavirus chromosomal integrated EP1 sequence of the parasitoid wasp Cotesia congregata (Braconidae, Microgastinae) at potential recombinase binding sites.

Cited by 72 publications

References 29 publications

When parasitic wasps hijacked viruses: genomic and functional evolution of polydnaviruses

When parasitic wasps hijacked viruses: genomic and functional evolution of polydnaviruses

Functional endogenous viral elements in the genome of the parasitoid wasp Cotesia congregata : insights into the evolutionary dynamics of bracoviruses

The few virus‐like genes of Cotesia congregata bracovirus

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