Excision of endosymbiotic bacteria from yeast under aging and starvation stresses

Heydari, Samira; Siavoshi, Farideh; Ebrahimi, Hoda; Sarrafnejad, Abdolfattah; Sharifi, Amir Houshang

doi:10.1016/j.meegid.2019.104141

Cited by 11 publications

(14 citation statements)

References 60 publications

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“…Although other authors have reported the presence of H. pylori within vacuoles of yeasts obtained from different sources, there is one study that proposes a stressing factor promotes the exit of intracellular bacteria from yeasts [6]. The present work is, to the best of our knowledge, the first report on the factors involved in the entry of H. pylori into yeasts, demonstrating that acidic pH values affect the entry of H. pylori into C. albicans.…”

Section: Discussionmentioning

confidence: 45%

“…Therefore, the endosymbiotic relationship between bacteria and hosts is a conserved phenomenon and has an important impact on the evolution of microorganisms [ 4 ]. For example, yeasts are highly sophisticated microorganisms with a notable capacity of change, which can adapt to environmental stress [ 5 ] and establish symbiotic relationships with certain microorganisms [ 6 ]. Furthermore, it has been found that Candida albicans may harbor Helicobacter pylori cells [ 3 , 6 , 7 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress

et al. 2020

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Yeasts can adapt to a wide range of pH fluctuations (2 to 10), while Helicobacter pylori, a facultative intracellular bacterium, can adapt to a range from pH 6 to 8. This work analyzed if H. pylori J99 can protect itself from acidic pH by entering into Candida albicans ATCC 90028. Growth curves were determined for H. pylori and C. albicans at pH 3, 4, and 7. Both microorganisms were co-incubated at the same pH values, and the presence of intra-yeast bacteria was evaluated. Intra-yeast bacteria-like bodies were detected using wet mounting, and intra-yeast binding of anti-H. pylori antibodies was detected using immunofluorescence. The presence of the H. pylori rDNA 16S gene in total DNA from yeasts was demonstrated after PCR amplification. H. pylori showed larger death percentages at pH 3 and 4 than at pH 7. On the contrary, the viability of the yeast was not affected by any of the pHs evaluated. H. pylori entered into C. albicans at all the pH values assayed but to a greater extent at unfavorable pH values (pH 3 or 4, p = 0.014 and p = 0.001, respectively). In conclusion, it is possible to suggest that H. pylori can shelter itself within C. albicans under unfavorable pH conditions.

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Section: Discussionmentioning

confidence: 45%

Section: Introductionmentioning

confidence: 99%

In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress

et al. 2020

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“…The extracellular and endocellular bacterial presence was observed 5 using light microscopic (supplementary video 2 and 3), phase contrast (Fig. 2A and 3A, Supplementary video 4), fluorescence microscopy (Figure 2B), confocal microscopy (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…3a). The yeast form was found to be surrounded by bacteria 5 . The bacterial motility was easily observed using a light microscope.…”

Section: Resultsmentioning

confidence: 99%

Moving Yeasts: Resolving the Mystery

Puri¹,

Chaudhari²,

Inamdar³

et al. 2022

Preprint

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We have recently discovered a novel dimorphic yeast Aureobasidium tremulum sp.nov. The species name tremulum indicates its trembling movement. With keen microscopic observations and various experimentation, we concluded that the movement is of two types, trembling movement, and actual displacement. The movement is because of two reasons; 1. Due to the dynamic movement of intracellular lipid granules, and; 2. Due to the movement of an intracellular bacterial population which is present as an endosymbiont. The mechanism of displacement has been hypothesized. The presence of both lipid granules and bacteria has been confirmed using microscopic analysis (Bright field, Phase contrast, confocal, Cryo-SEM, and HR-TEM). The bacterial presence was confirmed using whole-genome metagenomic analysis. Endosymbiotic bacteria have also been isolated and identified by 16s rRNA gene sequencing. Interestingly, some of them possibly are novel species. Also, the whole genome of A.tremulum has been announced. We also report on, restriction of the mode of bacterial mycophagy in presence of an antibiotic.

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“…It is generally associated with consistent accumulation of toxins at both cellular and molecular levels (Rizzo et al, 2014). Different studies have reported the use of murine, fruit flies (Drosophila melanogaster), nematodes (Caenorhabditis elegans) and the baker yeast (Saccharomyces cerevisiae) for aging models and cell growth studies (Neff et al, 2013;Qiao et al, 2019;Heydari et al, 2020;Olmedo et al, 2020). However, unicellular eukaryotes such as Saccharomyces cerevisiae is widely preferred because of the availability of their full sequenced genetic make-ups, many alternate knockout plasmids, insightful DNA replicating process, protein turn over, stress response with longevity and cell death mechanisms (Bitterman et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Capacities of Traditionally Formulated Thai Herbal Decoction and Its Effect on Cell Growth Using Saccharomyces cerevisiae Model

Adekoya¹,

Chusri²,

Ong³

et al. 2020

CMUJNS

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Polyherbal tea is widely consumed for rejuvenating of health in Thailand. Among them are Ya-Tri-Phigut (YTP), Ya-Tri-Kasornmas (YTK), Ya-Plook-Fithat (YPF), and Ya-Benjakul (YB). In addition, they have been listed as essential medicines with several others in the National List of Essential Medicines, Thailand. To ascertain their nutraceutical potentials, aqueous extracts of these herbal formulations were screened for their antioxidant activities. Extracts obtained from YTP showed higher yield (8.70 %) than YPF (6.40 %), YTK (6.30 %) and YB (6.30 %) (P <0.05). YTP also showed better antioxidant capacities than other tested formulations (P <0.05). Thus, it was used to test for its ability to support yeast cell. The extract (YTP) supported the yeast cell growth than the standard used called cryptotanshinone. Thus, YTP formulated herbal tonic can serve as a rich nutraceutical agent to combat oxidative stress and health related diseases.

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Excision of endosymbiotic bacteria from yeast under aging and starvation stresses

Cited by 11 publications

References 60 publications

In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress

In Vitro Incorporation of Helicobacter pylori into Candida albicans Caused by Acidic pH Stress

Moving Yeasts: Resolving the Mystery

Antioxidant Capacities of Traditionally Formulated Thai Herbal Decoction and Its Effect on Cell Growth Using Saccharomyces cerevisiae Model

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