By using an immunoperoxidase method with antiserotonin antiserum, the distribution of serotonin-immunoreactive cells in the carotid body region was investigated in chickens. The thyroid gland, cranial and caudal parathyroid glands, carotid body, and ultimobranchial gland of chickens were located along the common carotid artery as a continuous series and were supplied with branches arising from the artery. Almost all chief cells of the chick carotid bodies were immunoreactive for serotonin. Furthermore, numerous serotonin-immunoreactive cells were widely distributed in the adventitial connective tissue around those arteries that issued from the common carotid artery to supply each endocrine organ, i.e., the carotid body artery, the esophagotracheobronchial artery, the ascending esophageal artery, and the inferior thyroid artery. These arteries usually arose by one trunk from the lateral aspect of the middle portion of the common carotid artery. The serotonin cells were most numerous around the carotid body artery and were dispersed along the whole length of the artery. In addition, they were detected around the common trunk of each artery and the roots of the ascending esophageal artery, the inferior thyroid artery, and the esophagotracheobronchial artery. The serotonin cells were also distributed in the tunica media of the common carotid artery. In that place, they were concentrated around the origin of the common trunk of each artery and were scattered below the origin along the longitudinal axis and on the opposite side of the origin. The serotonin-immunoreactive cells distributed around and in the arteries may be involved in the control of blood flow and may have chemoreceptive properties.