2013
DOI: 10.1016/j.bpj.2013.06.002
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Exchangeable Colloidal AFM Probes for the Quantification of Irreversible and Long-Term Interactions

Abstract: An original method is presented to study single-colloid interaction with a substrate in liquid environment. Colloids, either in solution or adsorbed on a surface, are fixed by suction against the aperture of a microchanneled atomic force microscopy cantilever. Their adhesion to the substrate is measured, followed by their release via a short overpressure surge. Such colloid exchange procedure allows for 1), the quick variation of differently functionalized colloids within the same experiment; 2), the investiga… Show more

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Cited by 47 publications
(69 citation statements)
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“…Micropipette cantilevers with nominal spring constant of 0.2 N/m and an aperture of 4 μm in diameter were used. As an indenter, a polyethylene glycol coated polystyrene bead of 10 μm in diameter (Micromer #01-54-104, Micromod Partikeltechnologie GmbH, Germany) was immobilised at the aperture of the cantilever as follows54: beads at a density of 5 μg/mL were added into a petri dish containing culture medium. A bead was grabbed by applying a suction pressure of 700 mbar and the deflection sensitivity was calibrated.…”
Section: Methodsmentioning
confidence: 99%
“…Micropipette cantilevers with nominal spring constant of 0.2 N/m and an aperture of 4 μm in diameter were used. As an indenter, a polyethylene glycol coated polystyrene bead of 10 μm in diameter (Micromer #01-54-104, Micromod Partikeltechnologie GmbH, Germany) was immobilised at the aperture of the cantilever as follows54: beads at a density of 5 μg/mL were added into a petri dish containing culture medium. A bead was grabbed by applying a suction pressure of 700 mbar and the deflection sensitivity was calibrated.…”
Section: Methodsmentioning
confidence: 99%
“…For the FluidFM cantilevers used here, one finds a hydrodynamic resistance of R tot ≈ 5 × 10 17 (cf. calculation in Section S4 in the Supporting Information) . The volumetric flow rate Q through the aperture is Q = Δ p / R tot = V sol /Δ t .…”
mentioning
confidence: 99%
“…5. In particular, the microfluidics of FluidFM can be exploited to pick-up the sphere, and release it after few indentations (in case of contamination), without the need of gluing the sphere on the cantilever in advance, or changing the cantilever for every experiment [54,71]. On a different note, the interferometric read-out of the Chiaro nanoindenter results in a very quick and practical calibration and set-up of the experiment, without any special requirement for the sample holder (see for example the arrangement pictured in Fig.…”
Section: Discussionmentioning
confidence: 99%
“…The spring constant was determined in air by thermal tuning and the Sader's method [77] and the channel was filled with physiological solution containing 0.1 mg/ml of the blue fluorescent dye AMCA (7-amino-4-methylcoumarin, Sigma-Aldrich, USA) for blockage detection. Green fluorescent beads (Phosphorex Inc, USA) with 3µm to 4µm diameter were placed next to the confluent cell layer and attached to the cantilever tip by applying 800mbar suction pressure through the microchannel [71,78]. Indentations were performed at 1µm/s approach speed and up to 100nN force setpoint in grids of 5x5 points with 25µm pitch.…”
Section: Cell Indentation Measurements With Fluidfmmentioning
confidence: 99%