Excessive Reactive Oxygen Species and Exotic DNA Lesions as an Exploitable Liability

AbdulSalam, Safnas F.; Thowfeik, Fathima Shazna; Merino, Edward J.

doi:10.1021/acs.biochem.6b00703

Cited by 65 publications

(43 citation statements)

References 137 publications

(276 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[13,14] The interaction of plasma with cells may result in the oxidation of macromolecules and proteins, peroxidation of lipids, and damage to DNA which eventually interrupts cell signaling and metabolic processes. [15,16] The RONS produced by LTPs can increase the cells' oxidative stress leading to growth inhibition or cell death. [17][18][19] Many investigations have shown that there is a correlation between plasma produced RONS levels and cytotoxic effects.…”

Section: Introductionmentioning

confidence: 99%

Helium plasma jet interactions with water in well plates

Mohades

Lietz

Kruszelnicki

et al. 2019

Plasma Processes & Polymers

View full text Add to dashboard Cite

Plasma activation of liquids or cell cultures is a method for investigating the consequences of plasma produced reactive oxygen and nitrogen species (RONS) on living systems. The reproducible transfer of RONS, ions, electrons, and photons to the liquid is critical for determining reaction mechanisms and biological outcomes, and depends strongly on system parameters. A common in vitro method of plasma treatment of cells is a plasma jet directed into a well plate filled (or partially filled) with a liquid cell growth media. This method of treatment intrinsically has several environmental and geometrical factors that could lead to variability in the activation of the media. Uncontrolled or unreported geometrical and environmental factors that affect this transfer can, therefore, influence the reproducibility of measurements. In this paper, results from a numerical modeling investigation of a pulsed helium plasma jet interacting with water in a well plate are discussed while varying the height of the well‐plate rim. The height of the rim changes gas flow patterns, the ratio of He‐to‐air, and the water vapor content in the gas layer above the liquid. With a low rim, gas flowing from the jet stagnates on‐axis and flows radially outward with few vortices that recirculate reactants. With high rims, the gas flow is dominated by vortices and recirculation. With a low rim, the ionization wave (IW) from the jet strikes the liquid and proceeds as a surface IW across the water. With higher rims, the densities of helium and water vapor are higher above the liquid, which results in a volumetric propagation of the IW, initially producing higher densities of H2, HO2, OH, and H2O2. The end result is that for otherwise identical conditions, the densities of solvated H2O2aq and select reactive nitrogen species increase with rim height due to the vortices that recirculate reactants.

show abstract

Section: Introductionmentioning

confidence: 99%

Helium plasma jet interactions with water in well plates

Mohades

Lietz

Kruszelnicki

et al. 2019

Plasma Processes & Polymers

View full text Add to dashboard Cite

show abstract

“…Excessive ROS may induce DNA damage resulting in cancer cell apoptosis . Given differential responses of ROS modulating drugs and natural products between cancer and normal cells, selective DNA damage for selective apoptosis and killing may occur in cancer cells.…”

Section: Introductionmentioning

confidence: 99%

“…2 Therefore, several ROS modulating drugs and natural products have been developed to achieve oxidative stressbased selective cell killing for anticancer therapy. [3][4][5][6][7][8] Excessive ROS may induce DNA damage 9 resulting in cancer cell apoptosis. 10,11 Given differential responses of ROS modulating drugs and natural products between cancer and normal cells, 1 selective DNA damage for selective apoptosis and killing may occur in cancer cells.…”

Section: Introductionmentioning

confidence: 99%

4β‐Hydroxywithanolide E selectively induces oxidative DNA damage for selective killing of oral cancer cells

Tang

Huang

Wang

et al. 2017

Environmental Toxicology

View full text Add to dashboard Cite

Reactive oxygen species (ROS) induction had been previously reported in 4β-hydroxywithanolide (4βHWE)-induced selective killing of oral cancer cells, but the mechanism involving ROS and the DNA damage effect remain unclear. This study explores the role of ROS and oxidative DNA damage of 4βHWE in the selective killing of oral cancer cells. Changes in cell viability, morphology, ROS, DNA double strand break (DSB) signaling (γH2AX foci in immunofluorescence and DSB signaling in western blotting), and oxidative DNA damage (8-oxo-2'deoxyguanosine [8-oxodG]) were detected in 4βHWE-treated oral cancer (Ca9-22) and/or normal (HGF-1) cells. 4βHWE decreased cell viability, changed cell morphology and induced ROS generation in oral cancer cells rather than oral normal cells, which were recovered by a free radical scavenger N-acetylcysteine (NAC). For immunofluorescence, 4βHWE also accumulated more of the DSB marker, γH2AX foci, in oral cancer cells than in oral normal cells. For western blotting, DSB signaling proteins such as γH2AX and MRN complex (MRE11, RAD50, and NBS1) were overexpressed in 4βHWE-treated oral cancer cells in different concentrations and treatment time. In the formamidopyrimidine-DNA glycolyase (Fpg)-based comet assay and 8-oxodG-based flow cytometry, the 8-oxodG expressions were higher in 4βHWE-treated oral cancer cells than in oral normal cells. All the 4βHWE-induced DSB and oxidative DNA damage to oral cancer cells were recovered by NAC pretreatment. Taken together, the 4βHWE selectively induced DSB and oxidative DNA damage for the ROS-mediated selective killing of oral cancer cells.

show abstract

“…The HO . has sufficient oxidizing power to abstract an electron from a guanine base or from the ribose C−H bond, and this leads to DNA damage (Figure , black) . In this manuscript, we report a reagent that selectively eliminates HO .…”

Section: Figurementioning

confidence: 93%

“…Among DNA bases, guanine has the lowest redox potential. Under severe oxidative stress, guanine is oxidized to hyperoxidative lesions that are cytotoxic and mutagenic . One such lesion is 2,2,4‐triamino‐2 H ‐oxazol‐5‐one‐2′‐deoxyribonucleoside (dZ, oxazolone).…”

Section: Figurementioning

confidence: 99%

Self‐Cyclizing Antioxidants to Prevent DNA Damage Caused by Hydroxyl Radical

et al. 2017

Self Cite

View full text Add to dashboard Cite

Antioxidant therapy is a promising treatment strategy for protecting DNA from the damage caused by reactive oxygen species (ROS). Here, we report new self-cyclizing antioxidant reagents that are selective for the hydroxyl radical. Our mechanistic investigation revealed that the reagents react with three equivalents of oxidant in a cascade reaction to form a bicyclic final product. Among the reagents synthesized, 1 c showed favorable properties in vitro and in cellular studies. Using As O , which triggers ROS production, we showed that 1 c prevents formation of the guanine oxidation product 2,2,4-triamino-2H-oxazol-5-one-2'-deoxyribonucleoside and lowers cellular levels of reactive oxygen. The described self-cyclizing antioxidants are efficient, flexible, and tunable reagents with the potential to limit toxic oxidative stress.

show abstract

Excessive Reactive Oxygen Species and Exotic DNA Lesions as an Exploitable Liability

Cited by 65 publications

References 137 publications

Helium plasma jet interactions with water in well plates

Helium plasma jet interactions with water in well plates

4β‐Hydroxywithanolide E selectively induces oxidative DNA damage for selective killing of oral cancer cells

Self‐Cyclizing Antioxidants to Prevent DNA Damage Caused by Hydroxyl Radical

Contact Info

Product

Resources

About