We show that both the linear and the non-linear chiral models give an enhancement of the ππ cross section near the 2π threshold in the scalar-iso-scalar (I=J=0) channel in nuclear matter. The reduction of the chiral condensate, i.e., the partial chiral restoration in nuclear matter, is responsible for the enhancement in both cases. We extract an effective 4π-nucleon vertex which is responsible for the enhancement but has not been considered in the non-liear models for in-medium ππ interaction. Relation of this vertex and a next-to-leading order terms in the heavy-baryon chiral lagrangian, L (2) πN , is also discussed. 24.85.+p, 14.40.Cs Exploring possible evidence of partial restoration of chiral symmetry in hot and/or dense nuclear medium is an intriguing subject in hadron physics (see e.g., [1,2]). In particular, the softening of the scalar-iso-scalar (I = J = 0) fluctuation in matter could be a distinct signal of the partial restoration of chiral symmetry, as was originally pointed out in [3].In a recent paper [4], Shimizu and two of the present authors have shown, using the linear σ model (LσM), that the partial restoration of chiral symmetry in nuclear matter leads to an enhanced spectral function near the 2π threshold in the σ-channel. (The similar effect in hot hadronic plasma has been considered before [5,6].) They also suggested that the enhancement of the cross section in the reaction πA → π + π − A ′ near 2π threshold reported by the CHAOS collaboration [7], which was originally motivated by a possibility of a strong ππ correlation in matter [8], may be interpreted as an evidence of the partial restoration of chiral symmetry.In fact, the state-of-the-art calculations using the nonlinear chiral lagrangian together with πN many-body dynamics do not reproduce the cross sections in the I = 0 and I = 2 channels simultaneously [9]. In those calculations, the final state interactions of the emitted two pions in nuclei give rise to a slight enhancement of the cross section in the I = 0 channel, but is not sufficient to reproduce the experimental data. This indicates that some additional mechanism such as the partial restoration of chiral symmetry may be relevant for explaining the data. Such effect can be readily incorporated in the LσM as adopted in [4,10], while it is not obvious how to take into account the effect in the non-linear chiral models.The purposes of the present paper are twofold: Firstly, we show that the near-threshold enhancement in the I = J = 0 channel in nuclear matter takes place irrespective of the representations (linear or non-linear) of chiral symmetry, if the effect of partial restoration of chiral symmetry in the medium is properly incorporated. Secondly, we show that L (2) πN , which is a next-to-leading order term in the non-linear chiral lagrangian in the heavy-baryon formalism, gives relevant vertices for the near-threshold enhancement in the I=J=0 channel. Such vertices have not been taken into account in previous calculations of the in-medium ππ scattering in the non-linear...