Excess deposition of collagen in mammary glands of tamoxifen-treated Holstein heifers is associated with impaired mammary growth

Tucker, H.L.M.; Holdridge, Julie; Parsons, C.L.M.; Akers, R.M.

doi:10.1016/j.domaniend.2018.05.006

Cited by 4 publications

(4 citation statements)

References 27 publications

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“…Given the ability of Tmx to modulate ECM deposition, 20,26,27 and the impact of altered ECM on tendon function, we examined the effects of Tmx and CO pretreatment (WO) and sustained treatment (D0‐2q48) on gliding function. No changes in MTP flexion angle (Figure 4A) or GR (Figure 4B) were observed between Tmx‐ or CO‐treated tendon repairs from female mice, for both the WO and D0‐2q48 regimens.…”

Section: Resultsmentioning

confidence: 99%

“…The role of Tmx in extracellular matrix deposition and tissue fibrosis is strongly context‐dependent. For example, Tucker et al, 20 demonstrated that Tmx treatment doubled collagen deposition in the mammary stroma, resulting in altered ductal morphogenesis. In contrast, Tmx treatment was protective against renal fibrosis development following unilateral ureteral obstruction, with decreases in fibroblast activation and ECM deposition 27 .…”

Section: Discussionmentioning

confidence: 99%

“…However, a major limitation of this approach is that the effects of Tmx on the tendon‐healing process have not been rigorously characterized. Importantly, Tmx can exert either profibrotic or antifibrotic effects in both tissue and dose/regimen‐dependent contexts, 20,21 thus demonstrating the need to clearly understand the impact of Tmx on the healing process. Therefore, we were motivated to examine the effects of Tmx treatment and corn oil (CO) vehicle on functional and biomechanical properties of healing tendons, and to define potential sexual dimorphism in these metrics.…”

Section: Introductionmentioning

confidence: 99%

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Effects of tamoxifen on tendon homeostasis and healing: Considerations for the use of tamoxifen‐inducible mouse models

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Studentsova

Ackerman

et al. 2020

Journal Orthopaedic Research

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The use of tamoxifen‐inducible models of Cre recombinase in the tendon field is rapidly expanding, resulting in an enhanced understanding of tendon homeostasis and healing. However, the effects of tamoxifen on the tendon are not well‐defined, which is particularly problematic given that tamoxifen can have both profibrotic and antifibrotic effects in a tissue‐specific manner. Therefore, in the present study, we examined the effects of tamoxifen on tendon homeostasis and healing in male and female C57Bl/6J mice. Tamoxifen‐treated mice were compared to corn oil (vehicle)‐treated mice. In the “washout” treatment regimen, mice were treated with tamoxifen or corn oil for 3 days beginning 1 week prior to undergoing complete transection and surgical repair of the flexor digitorum longus tendon. In the second regimen, mice were treated with tamoxifen or corn oil beginning on the day of surgery, daily through day 2 postsurgery, and every 48 hours thereafter (D0‐2q48) until harvest. All repaired tendons and uninjured contralateral control tendons were harvested at day 14 postsurgery. Tamoxifen treatment had no effect on tendon healing in male mice, regardless of the treatment regimen, while Max load was significantly decreased in female repairs in the Tamoxifen washout group, relative to corn oil. In contrast, D0‐2q48 corn oil treatment in female mice led to substantial disruptions in tendon homeostasis, relative to washout corn oil treatment. Collectively, these data clearly define the functional effects of tamoxifen and corn oil treatment in the tendon and inform future use of tamoxifen‐inducible genetic models.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of tamoxifen on tendon homeostasis and healing: Considerations for the use of tamoxifen‐inducible mouse models

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Studentsova

Ackerman

et al. 2020

Journal Orthopaedic Research

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“…Interestingly, casein production is higher in bovine MECs seeded on floating collagen gels than on attached collagen gels [ 118 ]. In addition, excess deposition of type I collagen in mammary glands causes impaired mammary morphogenesis in cows [ 119 ]. However, it remains unclear whether and how much MECs contact with fibrillar collagens in vivo because the MECs are directly enclosed in basement membrane.…”

Section: Overview Of Mec Culture Modelsmentioning

confidence: 99%

Culture Models to Investigate Mechanisms of Milk Production and Blood-Milk Barrier in Mammary Epithelial Cells: a Review and a Protocol

Kobayashi

2023

J Mammary Gland Biol Neoplasia

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Mammary epithelial cells (MECs) are the only cell type that produces milk during lactation. MECs also form less-permeable tight junctions (TJs) to prevent the leakage of milk and blood components through the paracellular pathway (blood-milk barrier). Multiple factors that include hormones, cytokines, nutrition, and temperature regulate milk production and TJ formation in MECs. Multiple intracellular signaling pathways that positively and negatively regulate milk production and TJ formation have been reported. However, their regulatory mechanisms have not been fully elucidated. In addition, unidentified components that regulate milk production in MECs likely exist in foods, for example plants. Culture models of functional MECs that recapitulate milk production and TJs are useful tools for their study. Such models enable the elimination of indirect effects via cells other than MECs and allows for more detailed experimental conditions. However, culture models of MECs with inappropriate functionality may result in unphysiological reactions that never occur in lactating mammary glands in vivo. Here, I briefly review the physiological functions of alveolar MECs during lactation in vivo and culture models of MECs that feature milk production and less-permeable TJs, together with a protocol for establishment of MEC culture with functional TJ barrier and milk production capability using cell culture inserts.

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Distinct Expression Patterns of Fibrillar Collagen Types I, III, and V in Association with Mammary Gland Remodeling during Pregnancy, Lactation and Weaning

Tsutsui

Wakasa

Tsugami

et al. 2020

J Mammary Gland Biol Neoplasia

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Excess deposition of collagen in mammary glands of tamoxifen-treated Holstein heifers is associated with impaired mammary growth

Cited by 4 publications

References 27 publications

Effects of tamoxifen on tendon homeostasis and healing: Considerations for the use of tamoxifen‐inducible mouse models

Effects of tamoxifen on tendon homeostasis and healing: Considerations for the use of tamoxifen‐inducible mouse models

Culture Models to Investigate Mechanisms of Milk Production and Blood-Milk Barrier in Mammary Epithelial Cells: a Review and a Protocol

Distinct Expression Patterns of Fibrillar Collagen Types I, III, and V in Association with Mammary Gland Remodeling during Pregnancy, Lactation and Weaning

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