Excess copper in soil as a factor affecting bacterial spots caused by Xanthomonas vesicatoria in tomato plants; bio-interaction between two stress factors and their influence on plants

Maneva, Svetla; Bogatzevska, Nevena; Miteva, E.

doi:10.1007/s11738-008-0210-2

Cited by 6 publications

(6 citation statements)

References 27 publications

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“…Bacterial leaf spot caused by Xanthomonas campestris pv. vesicatoria (X.cv) and bacterial wilt caused by Ralstonia solanacearum are two devastating diseases of tomato (Maneva et al, 2009;Park & Han, 2017). X.cv can penetrate their host through the leaf stomata, sometimes hydathodes, and through a wounded site in humid conditions, causing small lesions that subsequently turn into small brown spots to appear on leaves, stems, and fruits (Park & Han, 2017).…”

Section: Slhyprp1 and Sldea1 Dual-gene Editing Imparts Bacterial Leaf...mentioning

confidence: 99%

CRISPR/Cas9-based genome editing and functional analysis of SlHyPRP1 and SlDEA1 genes of Solanum lycopersicum L. in imparting genetic tolerance to multiple stress factors

Saikia,

Debbarma

et al. 2024

Front. Plant Sci.

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CRISPR/Cas is a breakthrough genome editing system because of its precision, target specificity, and efficiency. As a speed breeding system, it is more robust than the conventional breeding and biotechnological approaches for qualitative and quantitative trait improvement. Tomato (Solanum lycopersicum L.) is an economically important crop, but its yield and productivity have been severely impacted due to different abiotic and biotic stresses. The recently identified SlHyPRP1 and SlDEA1 are two potential negative regulatory genes in response to different abiotic (drought and salinity) and biotic stress (bacterial leaf spot and bacterial wilt) conditions in S. lycopersicum L. The present study aimed to evaluate the drought, salinity, bacterial leaf spot, and bacterial wilt tolerance response in S. lycopersicum L. crop through CRISPR/Cas9 genome editing of SlHyPRP1 and SlDEA1 and their functional analysis. The transient single- and dual-gene SlHyPRP1 and SlDEA1 CRISPR-edited plants were phenotypically better responsive to multiple stress factors taken under the study. The CRISPR-edited SlHyPRP1 and SlDEA1 plants showed a higher level of chlorophyll and proline content compared to wild-type (WT) plants under abiotic stress conditions. Reactive oxygen species accumulation and the cell death count per total area of leaves and roots under biotic stress were less in CRISPR-edited SlHyPRP1 and SlDEA1 plants compared to WT plants. The study reveals that the combined loss-of-function of SlHyPRP1 along with SlDEA1 is essential for imparting significant multi-stress tolerance (drought, salinity, bacterial leaf spot, and bacterial wilt) in S. lycopersicum L. The main feature of the study is the detailed genetic characterization of SlDEA1, a poorly studied 8CM family gene in multi-stress tolerance, through the CRISPR/Cas9 gene editing system. The study revealed the key negative regulatory role of SlDEA1 that function together as an anchor gene with SlHyPRP1 in imparting multi-stress tolerance in S. lycopersicum L. It was interesting that the present study also showed that transient CRISPR/Cas9 editing events of SlHyPRP1 and SlDEA1 genes were successfully replicated in stably generated parent-genome-edited line (GEd0) and genome-edited first-generation lines (GEd1) of S. lycopersicum L. With these upshots, the study’s key findings demonstrate outstanding value in developing sustainable multi-stress tolerance in S. lycopersicum L. and other crops to cope with climate change.

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Section: Slhyprp1 and Sldea1 Dual-gene Editing Imparts Bacterial Leaf...mentioning

confidence: 99%

CRISPR/Cas9-based genome editing and functional analysis of SlHyPRP1 and SlDEA1 genes of Solanum lycopersicum L. in imparting genetic tolerance to multiple stress factors

Saikia,

Debbarma

et al. 2024

Front. Plant Sci.

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“…Control of the disease in the field is particularly cumbersome once infection occurs. Various chemicals were tested to control bacterial spot on tomato and pepper (Pham et al, 2004;Silva et al, 2006;Maneva et al, 2009;Nascimento et al, 2013). No chemicals were found to be efficient to control the disease in the field.…”

Section: At the Field Levelmentioning

confidence: 99%

Scientific Opinion on the pest categorisation of Xanthomonas campestris pv. vesicatoria (Doidge) Dye

Rossi¹

2014

EFS2

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The European Commission requested the EFSA Panel on Plant Health to perform the pest categorisation for Xanthomonas campestris pv. vesicatoria, which is the causal agent of bacterial spot of tomato and pepper. X. campestris pv. vesicatoria is not a single taxonomic entity, and four separate species have been described: X. vesicatoria, X. euvesicatoria, X. perforans and X. gardneri. These organisms can be accurately identified based on a range of discriminative methods. Detection methods are available for seeds. Among the four species described within X. campestris pv. vesicatoria, all except X. gardneri were reported to be present in the EU territory. The host plants (tomato and pepper) are cultivated throughout Europe and conditions are conducive to disease development in open fields in southern Europe and in greenhouses. The disease causes a range of symptoms on aerial parts of plants including fruits. Contaminated seeds and transplants are responsible for longdistance dissemination of the pathogen. Control is mainly based on prevention and exclusion. Extraction of seeds from fruit debris using fermentation and acid treatments and thermotherapy treatments were shown to be effective in reducing the bacterial load in seed lots. No methods and chemical control agents are available that effectively control xanthomonads in infected crops. Although no recent data are available on economic losses caused by these pathogens in the EU, the organisms are considered important bacterial pathogens of tomato and pepper. Infections resulting in up to 30 % losses have been reported. Xanthomonads causing bacterial spot of tomato and pepper meet all criteria defined in International Standard for Phytosanitary Measures (ISPM) 21 and they also meet all ISPM 11 criteria, although X. vesicatoria, X. euvesicatoria and X. perforans are present in the EU territory. After consideration of the evidence, the Panel reached the following conclusions:

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“…IGA had positive values when growth was increased and negative values when growth was inhibited. The relative weight (RW) for roots and shoots was calculated as the ratio between the biomass and the length (Maneva et al 2009). …”

Section: Biometrical Measurementsmentioning

confidence: 99%

“…Weakened plant defenses and increased susceptibility to pests and pathogens after prolonged exposure to abiotic stress conditions such as high metal levels, has been often observed (Miteva et al 2005;Mittler 2006;Atkinson & Urwin 2012). A plant's response may vary depending on metal level and place of pathogen penetration (Maneva et al 2009). According to Spann and Schumann (2010), unoptimal nutrient supply might increase disease susceptibility by affecting the formation of mechanical barriers or the synthesis of natural defense compounds so that plants with an optimal nutritional status usually have the highest resistance to diseases.…”

Section: Combined Effect Of Excess Cu and Fusarium Infectionmentioning

confidence: 99%

Separate and combined effects of excess copper and Fusarium culmorum infection on growth and antioxidative enzymes in wheat (Triticum aestivum L.) plants

Nenova

Bogoeva

2013

Journal of Plant Interactions

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The effect of stress combinations on plants cannot be extrapolated from the response to each of the applied stressors. Greenhouse experiments were carried out on soil to which copper ions were introduced at four concentrations (0, 150, 400, and 600 mg kg(1 ). Copper treatments without or with Fusarium infection were established. Both stress factors, applied separately or together inhibited growth with the exception of the lowest Cu concentration, which stimulated growth of healthy plants. Depending on concentration, Cu did not change or increased the activity of root peroxidase and leaf catalase, and decreased ascorbate peroxidase (APO) activity in leaves and roots. Infection increased the activities of the enzymes with exception of root APO. The simultaneous presence of these two stress factors modified their individual effects. Generally, the stress combination aggravated the plant status though an opposite trend was observed in some cases.

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Excess copper in soil as a factor affecting bacterial spots caused by Xanthomonas vesicatoria in tomato plants; bio-interaction between two stress factors and their influence on plants

Cited by 6 publications

References 27 publications

CRISPR/Cas9-based genome editing and functional analysis of SlHyPRP1 and SlDEA1 genes of Solanum lycopersicum L. in imparting genetic tolerance to multiple stress factors

CRISPR/Cas9-based genome editing and functional analysis of SlHyPRP1 and SlDEA1 genes of Solanum lycopersicum L. in imparting genetic tolerance to multiple stress factors

Scientific Opinion on the pest categorisation of Xanthomonas campestris pv. vesicatoria (Doidge) Dye

Separate and combined effects of excess copper and Fusarium culmorum infection on growth and antioxidative enzymes in wheat (Triticum aestivum L.) plants

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