Excellent waste biomass-degrading performance of Trichoderma asperellum T-1 during submerged fermentation

Wang, Qun; Liang, Chen; Yu, Daobing; Lin, Hui; Shen, Qi; Zhao, Yanfang

doi:10.1016/j.scitotenv.2017.07.212

Cited by 20 publications

(7 citation statements)

References 33 publications

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“…After treatment with Y7, the length and width of the switchgrass were substantially decreased. Similar structural changes were also observed in the hydrolyzed wheat straw (Kshirsagar et al, 2016) and switchgrass (Wang et al, 2017). The SEM investigations observed a good growth and attachment of G. thermodenitrificans Y7 on the surface of the switchgrass.…”

Section: Discussionsupporting

confidence: 75%

Isolation of Thermostable Lignocellulosic Bacteria From Chicken Manure Compost and a M42 Family Endocellulase Cloning From Geobacillus thermodenitrificans Y7

Zhao

Meng

et al. 2020

Front. Microbiol.

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The composting ecosystem provides a potential resource for finding new microorganisms with the capability for cellulose degradation. In the present study, Congo red method was used for the isolating of thermostable lignocellulose-degrading bacteria from chicken manure compost. A thermophilic strain named as Geobacillus thermodenitrificans Y7 with acid-resident property was successfully isolated and employed to degrade raw switchgrass at 60 • C for 5 days, which resulted in the final degradation rates of cellulose, xylan, and acid-insoluble lignin as 18.64, 12.96, and 17.21%, respectively. In addition, GC-MS analysis about aromatic degradation affirm the degradation of lignin by G. thermodenitrificans Y7. Moreover, an endocellulase gene belong to M42 family was successfully cloned from G. thermodenitrificans Y7 and expressed in Escherichia coli BL21. Recombinant enzyme Cel-9 was purified by Ni-NTA column based the His-tag, and the molecular weight determined as 40.4 kDa by SDA-PAGE. The characterization of the enzyme Cel-9 indicated that the maximum enzyme activity was realized at 50 • C and pH 8.6 and, Mn 2+ could greatly improve the CMCase enzyme activity of Cel-9 at 10 mM, which was followed by Fe 2+ and Co 2+. Besides, it also found that the β-1,3-1,4, β-1,3, β-1,4, and β-1,6 glucan linkages all could be hydrolyzed by enzyme Cel-9. Finally, during the application of enzyme Cel-9 to switchgrass, the saccharification rates achieved to 1.81 ± 0.04% and 2.65 ± 0.03% for 50 and 100% crude enzyme, respectively. All these results indicated that both the strain G. thermodenitrificans Y7 and the recombinant endocellulase Cel-9 have the potential to be applied to the biomass industry.

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Section: Discussionsupporting

confidence: 75%

Isolation of Thermostable Lignocellulosic Bacteria From Chicken Manure Compost and a M42 Family Endocellulase Cloning From Geobacillus thermodenitrificans Y7

Zhao

Meng

et al. 2020

Front. Microbiol.

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“…The major cellulases involved in lignocellulose degradation are known to include cellobiohydrolase, β-glucosidase, xylanase, and carboxymethyl cellulase. As it was also found in our previous research that the β-glucosidase activity, xylanase activity, and carboxymethyl cellulase activity of T. asperellum T-1 were actually high (Wang et al 2017), it could be indicated that the relatively weak filter paper cellulase activity might be caused by the insufficient secretion of cellobiohydrolase. Thus, we chose the cbh gene to be overexpressed to improve the cellulase production of T. asperellum T-1.…”

Section: Construction Of the Genetic Transformation System Of T Aspesupporting

confidence: 71%

“…After centrifugation at 12,000 rpm for 1 min, the supernatant was collected and stored at − 20°C. Filter paper cellulase activity (FPase), endoglucanase activity (CMCase), βglucosidase activity, and xylanase activity were analyzed using the previously reported method (Wang et al 2017). All of the fermentation experiments were performed in triplicates, and the obtained samples were further analyzed in triplicates.…”

Section: Growth Of Positive Transformantsmentioning

confidence: 99%

“…Even the excellent waste biomass-degrading performance of T. asperellum T-1 has been investigated in our previous research by comparison with Trichoderma reesei QM6a (Wang et al 2017); it was also found that the filter paper cellulase activity, which can reflect the total activity of cellulose, was relatively weak. As an effective biological control species against many pathogenic microorganisms, T. asperellum received considerable attention (Keswani et al 2014).…”

Section: Introductionmentioning

confidence: 95%

“…In our previous research, a strain of Trichoderma asperellum, named T-1, was found to be able to secrete cellulase with enhanced hydrolysis abilities. Most importantly, this strain can ferment the natural materials without pretreatment, showing a great potential in the degradation of natural waste lignocellulosic material (Wang et al 2015;Wang et al 2017). Studies about T. asperellum have reported its potential in glycoside hydrolase (GH) production, and those hydrolases can degrade the carbohydrates in the plant cell walls to release bioactive polymers, proteins, and simple sugars (Selvamani et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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The overexpression of one single cbh gene making Trichoderma asperellum T-1 a better cellulase producer

et al. 2019

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Trichoderma asperellum T-1, a traditional bio-control strain, is previously found to be potentially useful in the degradation of natural waste lignocellulose as it can ferment the natural materials without pretreatment. Many problems caused by substrate pretreatment can be therefore avoided. In this study, we intended to engineer a new strain to enhance its lignocellulose degradation ability by modifying the genome of T. asperellum T-1. A genetic transformation system mediated by Agrobacterium tumefaciens AGL-1 (ATMT) was constructed on T. asperellum T-1. On this basis, the overexpressed strain was produced by transforming a recombinant cellobiosidase gene (cbh) under the control of inducible promoter of endo-1, 4-β-xylanase gene, into wild-type T. asperellum T-1. After resistance screening, multiple transmission, growth comparison, and enzyme activity determination, four transformants (M1, M2, M5, and M6) were obtained. Filter paper cellulase activity of these transformants reached, respectively, 36.2%, 30.6%, 32.9%, and 42.7% higher than the wild-type strain. Most importantly, the CMCase, β-glucosidase and xylanase activity were also increased, although only one cbh gene was overexpressed. This work indicated that the enhancement of cellulase production ability of T. asperellum T-1 can be promisingly feasible by genetic modification. And the xylanase gene's promoter can be effectively used in genetic modification to promote T. asperellum T-1 to be more effectively used in lignocellulose degradation.

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Extracellular Carbohydrate-Active Enzymes of Trichoderma and Their Role in the Bioconversion of Non-edible Biomass to Biofuel

Sharma

Salwan

2019

Recent Advancement in White Biotechnology Through Fungi

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Excellent waste biomass-degrading performance of Trichoderma asperellum T-1 during submerged fermentation

Cited by 20 publications

References 33 publications

Isolation of Thermostable Lignocellulosic Bacteria From Chicken Manure Compost and a M42 Family Endocellulase Cloning From Geobacillus thermodenitrificans Y7

Isolation of Thermostable Lignocellulosic Bacteria From Chicken Manure Compost and a M42 Family Endocellulase Cloning From Geobacillus thermodenitrificans Y7

The overexpression of one single cbh gene making Trichoderma asperellum T-1 a better cellulase producer

Extracellular Carbohydrate-Active Enzymes of Trichoderma and Their Role in the Bioconversion of Non-edible Biomass to Biofuel

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