Examining the Influence of Low-Dose Tissue Plasminogen Activator on Microbubble-Mediated Forward-Viewing Intravascular Sonothrombolysis

Goel, Leela; Wu, Huaiyu; Kim, Howuk; Zhang, Bohua; Kim, Jin-Wook; Dayton, Paul A.; Xu, Zhen; Jiang, Xiaoning

doi:10.1016/j.ultrasmedbio.2020.03.012

Cited by 21 publications

(9 citation statements)

References 35 publications

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“…Our group has developed a forward-viewing intravascular (FVI) sub-megahertz transducer for sonothrombolysis, which is effective for tPA and MB-mediated sonothrombolysis in unretracted clot models ( Fig. 1a) [27][28][29] . An intravascular system does not have the treatment location limitation of using an external transducer.…”

Section: Fvi Transducer For Sonothrombolysismentioning

confidence: 99%

“…Previous studies from our group have determined optimal transducer excitation parameters for MB-mediated sonothrombolysis for unretracted clots 27 . We then were able to combine MBs with low dose of tPA to further enhance clot lysis outcomes in unretracted clots, and further understand the synergistic effects of combining tPA with MBs when using our FVI sonothrombolysis transducer 29 .…”

Section: Fvi Transducer For Sonothrombolysismentioning

confidence: 99%

“…The FVI transducer was fabricated similarly to previous studies 27,29 . The transducer was composed of seven stacked layers of PZT-5A, E-solder 3022 bonding layers, and an alumina epoxy matching layer.…”

Section: Fvi Transducermentioning

confidence: 99%

“…The minimum PNP necessary for ND activation was determined using PCD, performed similarly to our previous study 29 . The PNP of the FVI transducer was varied from 0.3 MPa to 1.2 MPa.…”

Section: Pcd For Nanodroplet Activationmentioning

confidence: 99%

“…In order to enhance retracted clot lysis, we combined NDs with a low dose of tPA. Given the success of MB mediated sonothrombolysis with a lowdose tPA in unretracted clots, we chose to combine NDmediated sonothrombolysis with a low dose tPA 29 . We compared ND + tPA-mediated sonothrombolysis to a control of PBS alone without ultrasound, tPA treatment alone without ultrasound, tPA with ultrasound, NDmediated thrombolysis, MB mediated sonothrombolysis, and MB + tPA-mediated sonothrombolysis.…”

Section: Clot Lysismentioning

confidence: 99%

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Nanodroplet-mediated catheter-directed sonothrombolysis of retracted blood clots

Goel

Zhang

et al. 2021

Microsyst Nanoeng

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One major challenge in current microbubble (MB) and tissue plasminogen activator (tPA)-mediated sonothrombolysis techniques is effectively treating retracted blood clots, owing to the high density and low porosity of retracted clots. Nanodroplets (NDs) have the potential to enhance retracted clot lysis owing to their small size and ability to penetrate into retracted clots to enhance drug delivery. For the first time, we demonstrate that a sub-megahertz, forward-viewing intravascular (FVI) transducer can be used for ND-mediated sonothrombolysis, in vitro. In this study, we determined the minimum peak negative pressure to induce cavitation with low-boiling point phase change nanodroplets and clot lysis. We then compared nanodroplet mediated sonothrombolysis to MB and tPA mediate techniques. The clot lysis as a percent mass decrease in retracted clots was 9 ± 8%, 9 ± 5%, 16 ± 5%, 14 ± 9%, 17 ± 9%, 30 ± 8%, and 40 ± 9% for the control group, tPA alone, tPA + US, MB + US, MB + tPA + US, ND + US, and ND + tPA + US groups, respectively. In retracted blood clots, combined ND- and tPA-mediated sonothrombolysis was able to significantly enhance retracted clot lysis compared with traditional MB and tPA-mediated sonothrombolysis techniques. Combined nanodroplet with tPA-mediated sonothrombolysis may provide a feasible strategy for safely treating retracted clots.

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Section: Fvi Transducer For Sonothrombolysismentioning

confidence: 99%

Section: Fvi Transducer For Sonothrombolysismentioning

confidence: 99%

Section: Fvi Transducermentioning

confidence: 99%

“…The minimum PNP necessary for ND activation was determined using PCD, performed similarly to our previous study 29 . The PNP of the FVI transducer was varied from 0.3 MPa to 1.2 MPa.…”

Section: Pcd For Nanodroplet Activationmentioning

confidence: 99%

Section: Clot Lysismentioning

confidence: 99%

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